VAMP8-mediated NOX2 recruitment to endosomes is necessary for antigen release

Dingjan I, Paardekooper LM, Verboogen DRJ, Fischer von Mollard G, ter Beest M, van den Bogaart G (2017)
European Journal of Cell Biology 96(7): 705-714.

Download
No fulltext has been uploaded. References only!
Journal Article | Original Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ;
Abstract
Cross-presentation of foreign antigen in major histocompatibility complex (MHC) class I by dendritic cells (DCs) requires activation of the NADPH-oxidase NOX2 complex. We recently showed that NOX2 is recruited to phagosomes by the SNARE protein VAMP8 where NOX2-produced reactive oxygen species (ROS) cause lipid oxidation and membrane disruption, promoting antigen translocation into the cytosol for cross-presentation. In this study, we extend these findings by showing that VAMP8 is also involved in NOX2 trafficking to endosomes. Moreover, we demonstrate in both human and mouse DCs that absence of VAMP8 leads to decreased ROS production, lipid peroxidation and antigen translocation, and that this impairs cross-presentation. In contrast, knockdown of VAMP8 did not affect recruitment of MHC class I and the transporter associated with antigen processing 1 (TAP1) to phagosomes, although surface levels of MHC class I were reduced. Thus, in addition to a secretory role, VAMP8-mediates trafficking of NOX2 to endosomes and phagosomes and this promotes induction of cytolytic T cell immune responses. (C) 2017 The Authors. Published by Elsevier GmbH.
Publishing Year
ISSN
eISSN
PUB-ID

Cite this

Dingjan I, Paardekooper LM, Verboogen DRJ, Fischer von Mollard G, ter Beest M, van den Bogaart G. VAMP8-mediated NOX2 recruitment to endosomes is necessary for antigen release. European Journal of Cell Biology. 2017;96(7):705-714.
Dingjan, I., Paardekooper, L. M., Verboogen, D. R. J., Fischer von Mollard, G., ter Beest, M., & van den Bogaart, G. (2017). VAMP8-mediated NOX2 recruitment to endosomes is necessary for antigen release. European Journal of Cell Biology, 96(7), 705-714. doi:10.1016/j.ejcb.2017.06.007
Dingjan, I., Paardekooper, L. M., Verboogen, D. R. J., Fischer von Mollard, G., ter Beest, M., and van den Bogaart, G. (2017). VAMP8-mediated NOX2 recruitment to endosomes is necessary for antigen release. European Journal of Cell Biology 96, 705-714.
Dingjan, I., et al., 2017. VAMP8-mediated NOX2 recruitment to endosomes is necessary for antigen release. European Journal of Cell Biology, 96(7), p 705-714.
I. Dingjan, et al., “VAMP8-mediated NOX2 recruitment to endosomes is necessary for antigen release”, European Journal of Cell Biology, vol. 96, 2017, pp. 705-714.
Dingjan, I., Paardekooper, L.M., Verboogen, D.R.J., Fischer von Mollard, G., ter Beest, M., van den Bogaart, G.: VAMP8-mediated NOX2 recruitment to endosomes is necessary for antigen release. European Journal of Cell Biology. 96, 705-714 (2017).
Dingjan, Ilse, Paardekooper, Laurent M., Verboogen, Danielle R. J., Fischer von Mollard, Gabriele, ter Beest, Martin, and van den Bogaart, Geert. “VAMP8-mediated NOX2 recruitment to endosomes is necessary for antigen release”. European Journal of Cell Biology 96.7 (2017): 705-714.
This data publication is cited in the following publications:
This publication cites the following data publications:

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 28688576
PubMed | Europe PMC

Search this title in

Google Scholar