Aescin Incorporation and Nanodomain Formation in DMPC Model Membranes

Sreij R, Dargel C, Hernandez Moleiro L, Monroy F, Hellweg T (2017)
LANGMUIR 33(43): 12351-12361.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Abstract / Bemerkung
The saponin aescin from the horse chestnut tree is a natural surfactant well-known to self-assemble as oriented-aggregates at fluid interfaces. Using model membranes in the form of lipid vesicles and Langmuir monolayers, we study the mixing properties of aescin with the phase-segregating phospholipid 1,2-dimyristoyl-sn-glycero-phosphocholine (DMPC). The binary membranes are experimentally studied on different length scales ranging from the lipid headgroup area to the macroscopic scale using small-angle X-ray scattering (SAXS), photon correlation spectroscopy (PCS), and differential scanning calorimetry (DSC) with binary bilayer vesicles and Langmuir tensiometry (LT) with lipid monolayers spread on the surface of aescin solutions. The binary interaction was found to strongly depend on aescin concentration in two well differentiated concentration regimes. Below 7 mol %, the results reveal phase segregation of nanometer-sized aescin-rich domains in an aescin-poor continuous bilayer. Above this concentration, aescin-aescin interactions dominate, which inhibit vesicle formation but lead to the formation of new membrane aggregates of smaller sizes. From LT studies in monolayers, the interaction of aescin with DMPC was shown to be stronger in the condensed phase than in the liquid expanded phase. Furthermore, a destructuring role was revealed for aescin on phospholipid membranes, similar to the fluidizing effect of cholesterol and nonsteroidal anti-inflammatory drugs (NSAIDs) on lipid bilayers.
Erscheinungsjahr
Zeitschriftentitel
LANGMUIR
Band
33
Zeitschriftennummer
43
Seite
12351-12361
ISSN
PUB-ID

Zitieren

Sreij R, Dargel C, Hernandez Moleiro L, Monroy F, Hellweg T. Aescin Incorporation and Nanodomain Formation in DMPC Model Membranes. LANGMUIR. 2017;33(43):12351-12361.
Sreij, R., Dargel, C., Hernandez Moleiro, L., Monroy, F., & Hellweg, T. (2017). Aescin Incorporation and Nanodomain Formation in DMPC Model Membranes. LANGMUIR, 33(43), 12351-12361. doi:10.1021/acs.langmuir.7b02933
Sreij, R., Dargel, C., Hernandez Moleiro, L., Monroy, F., and Hellweg, T. (2017). Aescin Incorporation and Nanodomain Formation in DMPC Model Membranes. LANGMUIR 33, 12351-12361.
Sreij, R., et al., 2017. Aescin Incorporation and Nanodomain Formation in DMPC Model Membranes. LANGMUIR, 33(43), p 12351-12361.
R. Sreij, et al., “Aescin Incorporation and Nanodomain Formation in DMPC Model Membranes”, LANGMUIR, vol. 33, 2017, pp. 12351-12361.
Sreij, R., Dargel, C., Hernandez Moleiro, L., Monroy, F., Hellweg, T.: Aescin Incorporation and Nanodomain Formation in DMPC Model Membranes. LANGMUIR. 33, 12351-12361 (2017).
Sreij, Ramsia, Dargel, Carina, Hernandez Moleiro, Lara, Monroy, Francisco, and Hellweg, Thomas. “Aescin Incorporation and Nanodomain Formation in DMPC Model Membranes”. LANGMUIR 33.43 (2017): 12351-12361.

1 Zitation in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

DMPC vesicle structure and dynamics in the presence of low amounts of the saponin aescin.
Sreij R, Dargel C, Geisler P, Hertle Y, Radulescu A, Pasini S, Perez J, Moleiro LH, Hellweg T., Phys Chem Chem Phys 20(14), 2018
PMID: 29505043

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 28985678
PubMed | Europe PMC

Suchen in

Google Scholar