Metabolite profiling at the cellular and subcellular level reveals metabolites associated with salinity tolerance in sugar beet

Hossain MS, Persicke M, ElSayed AI, Kalinowski J, Dietz K-J (2017)
Journal of Experimental Botany 68(21-22): 5961-5976.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
; ; ; ;
Abstract / Bemerkung
Sugar beet is among the most salt-tolerant crops. This study aimed to investigate the metabolic adaptation of sugar beet to salt stress at the cellular and subcellular levels. Seedlings were grown hydroponically and subjected to stepwise increases in salt stress up to 300 mM NaCl. Highly enriched fractions of chloroplasts were obtained by nonaqueous fractionation using organic solvents. Total leaf metabolites and metabolites in chloroplasts were profiled at 3 h and 14 d after reaching the maximum salinity stress of 300 mM NaCl. Metabolite profiling by gas chromatography- mass spectrometry (GC-MS) resulted in the identification of a total of 83 metabolites in leaves and chloroplasts under control and stress conditions. There was a lower abundance of Calvin cycle metabolites under salinity whereas there was a higher abundance of oxidative pentose phosphate cycle metabolites such as 6-phosphogluconate. Accumulation of ribose-5-phosphate and ribulose-5-phosphate coincided with limitation of carbon fixation by ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). Increases in glycolate and serine levels indicated that photorespiratory metabolism was stimulated in salt-stressed sugar beet. Compatible solutes such as proline, mannitol, and putrescine accumulated mostly outside the chloroplasts. Within the chloroplast, putrescine had the highest relative level and probably assisted in the acclimation of sugar beet to high salinity stress. The results provide new information on the contribution of chloroplasts and the extra-chloroplast space to salinity tolerance via metabolic adjustment in sugar beet.
Erscheinungsjahr
Zeitschriftentitel
Journal of Experimental Botany
Band
68
Zeitschriftennummer
21-22
Seite
5961-5976
ISSN
eISSN
PUB-ID

Zitieren

Hossain MS, Persicke M, ElSayed AI, Kalinowski J, Dietz K-J. Metabolite profiling at the cellular and subcellular level reveals metabolites associated with salinity tolerance in sugar beet. Journal of Experimental Botany. 2017;68(21-22):5961-5976.
Hossain, M. S., Persicke, M., ElSayed, A. I., Kalinowski, J., & Dietz, K. - J. (2017). Metabolite profiling at the cellular and subcellular level reveals metabolites associated with salinity tolerance in sugar beet. Journal of Experimental Botany, 68(21-22), 5961-5976. doi:10.1093/jxb/erx388
Hossain, M. S., Persicke, M., ElSayed, A. I., Kalinowski, J., and Dietz, K. - J. (2017). Metabolite profiling at the cellular and subcellular level reveals metabolites associated with salinity tolerance in sugar beet. Journal of Experimental Botany 68, 5961-5976.
Hossain, M.S., et al., 2017. Metabolite profiling at the cellular and subcellular level reveals metabolites associated with salinity tolerance in sugar beet. Journal of Experimental Botany, 68(21-22), p 5961-5976.
M.S. Hossain, et al., “Metabolite profiling at the cellular and subcellular level reveals metabolites associated with salinity tolerance in sugar beet”, Journal of Experimental Botany, vol. 68, 2017, pp. 5961-5976.
Hossain, M.S., Persicke, M., ElSayed, A.I., Kalinowski, J., Dietz, K.-J.: Metabolite profiling at the cellular and subcellular level reveals metabolites associated with salinity tolerance in sugar beet. Journal of Experimental Botany. 68, 5961-5976 (2017).
Hossain, M. Sazzad, Persicke, Marcus, ElSayed, Abdelaleim Ismail, Kalinowski, Jörn, and Dietz, Karl-Josef. “Metabolite profiling at the cellular and subcellular level reveals metabolites associated with salinity tolerance in sugar beet”. Journal of Experimental Botany 68.21-22 (2017): 5961-5976.

3 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Dynamics of metabolic responses to periods of combined heat and drought in Arabidopsis thaliana under ambient and elevated atmospheric CO2.
Zinta G, AbdElgawad H, Peshev D, Weedon JT, Van den Ende W, Nijs I, Janssens IA, Beemster GTS, Asard H., J Exp Bot 69(8), 2018
PMID: 29462345

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 29140437
PubMed | Europe PMC

Suchen in

Google Scholar