Comparative proteomics of chloroplast envelopes from C(3) and C(4) plants reveals specific adaptations of the plastid envelope to C(4) photosynthesis and candidate proteins required for maintaining C(4) metabolite fluxes

Bräutigam A, Hofmann-Benning S, Weber APM (2008)
Plant Physiology 148(1): 568-579.

Download
OA 494.52 KB
Journal Article | Original Article | Published | English
Author
; ;
Abstract
C(4) plants have up to 10-fold higher apparent CO(2) assimilation rates than the most productive C(3) plants. This requires higher fluxes of metabolic intermediates across the chloroplast envelope membranes of C(4) plants in comparison with those of C(3) plants. In particular, the fluxes of metabolites involved in the biochemical inorganic carbon pump of C(4) plants, such as malate, pyruvate, oxaloacetate, and phosphoenolpyruvate, must be considerably higher in C(4) plants because they exceed the apparent rate of photosynthetic CO(2) assimilation, whereas they represent relatively minor fluxes in C(3) plants. While the enzymatic steps involved in the C(4) biochemical inorganic carbon pump have been studied in much detail, little is known about the metabolite transporters in the envelope membranes of C(4) chloroplasts. In this study, we used comparative proteomics of chloroplast envelope membranes from the C(3) plant pea (Pisum sativum) and mesophyll cell chloroplast envelopes from the C(4) plant maize (Zea mays) to analyze the adaptation of the mesophyll cell chloroplast envelope proteome to the requirements of C(4) photosynthesis. We show that C(3)- and C(4)- type chloroplasts have qualitatively similar but quantitatively very different chloroplast envelope membrane proteomes. In particular, translocators involved in the transport of triosephosphate and phosphoenolpyruvate as well as two outer envelope porins are much more abundant in C(4) plants. Several putative transport proteins have been identified that are highly abundant in C(4) plants but relatively minor in C(3) envelopes. These represent prime candidates for the transport of C(4) photosynthetic intermediates, such as pyruvate, oxaloacetate, and malate.
Publishing Year
ISSN
PUB-ID

Cite this

Bräutigam A, Hofmann-Benning S, Weber APM. Comparative proteomics of chloroplast envelopes from C(3) and C(4) plants reveals specific adaptations of the plastid envelope to C(4) photosynthesis and candidate proteins required for maintaining C(4) metabolite fluxes. Plant Physiology. 2008;148(1):568-579.
Bräutigam, A., Hofmann-Benning, S., & Weber, A. P. M. (2008). Comparative proteomics of chloroplast envelopes from C(3) and C(4) plants reveals specific adaptations of the plastid envelope to C(4) photosynthesis and candidate proteins required for maintaining C(4) metabolite fluxes. Plant Physiology, 148(1), 568-579. doi:10.1104/pp.108.121012
Bräutigam, A., Hofmann-Benning, S., and Weber, A. P. M. (2008). Comparative proteomics of chloroplast envelopes from C(3) and C(4) plants reveals specific adaptations of the plastid envelope to C(4) photosynthesis and candidate proteins required for maintaining C(4) metabolite fluxes. Plant Physiology 148, 568-579.
Bräutigam, A., Hofmann-Benning, S., & Weber, A.P.M., 2008. Comparative proteomics of chloroplast envelopes from C(3) and C(4) plants reveals specific adaptations of the plastid envelope to C(4) photosynthesis and candidate proteins required for maintaining C(4) metabolite fluxes. Plant Physiology, 148(1), p 568-579.
A. Bräutigam, S. Hofmann-Benning, and A.P.M. Weber, “Comparative proteomics of chloroplast envelopes from C(3) and C(4) plants reveals specific adaptations of the plastid envelope to C(4) photosynthesis and candidate proteins required for maintaining C(4) metabolite fluxes”, Plant Physiology, vol. 148, 2008, pp. 568-579.
Bräutigam, A., Hofmann-Benning, S., Weber, A.P.M.: Comparative proteomics of chloroplast envelopes from C(3) and C(4) plants reveals specific adaptations of the plastid envelope to C(4) photosynthesis and candidate proteins required for maintaining C(4) metabolite fluxes. Plant Physiology. 148, 568-579 (2008).
Bräutigam, Andrea, Hofmann-Benning, Susanne, and Weber, Andreas P. M. “Comparative proteomics of chloroplast envelopes from C(3) and C(4) plants reveals specific adaptations of the plastid envelope to C(4) photosynthesis and candidate proteins required for maintaining C(4) metabolite fluxes”. Plant Physiology 148.1 (2008): 568-579.
All files available under the following license(s):
Copyright Statement:
This Item is protected by copyright and/or related rights. [...]
Main File(s)
Access Level
OA Open Access
Last Uploaded
2017-12-18T10:50:29Z

This data publication is cited in the following publications:
This publication cites the following data publications:

57 Citations in Europe PMC

Data provided by Europe PubMed Central.

Intracellular metabolite transporters in plants.
Linka N, Weber AP., Mol Plant 3(1), 2010
PMID: 20038549
The regulation of gene expression required for C4 photosynthesis.
Hibberd JM, Covshoff S., Annu Rev Plant Biol 61(), 2010
PMID: 20192753
Cell-type-specific differentiation of chloroplasts in C4 plants.
Majeran W, van Wijk KJ., Trends Plant Sci 14(2), 2009
PMID: 19162526
Plant proteomics update (2007-2008): Second-generation proteomic techniques, an appropriate experimental design, and data analysis to fulfill MIAPE standards, increase plant proteome coverage and expand biological knowledge.
Jorrín-Novo JV, Maldonado AM, Echevarría-Zomeño S, Valledor L, Castillejo MA, Curto M, Valero J, Sghaier B, Donoso G, Redondo I., J Proteomics 72(3), 2009
PMID: 19367730

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 18599648
PubMed | Europe PMC

Search this title in

Google Scholar