A plastidial sodium-dependent pyruvate transporter

Furumoto T, Yamaguchi T, Ohshima-Ichie Y, Nakamura M, Tsuchida-Iwata Y, Shimamura M, Ohnishi J, Hata S, Gowik U, Westhoff P, Bräutigam A, et al. (2011)
Nature 476(7361): 472-U131.

OA 818.67 KB
Journal Article | Original Article | Published | English
; ; ; ; ; ; ; ; ; ; ;
Pyruvate serves as a metabolic precursor for many plastid-localized biosynthetic pathways, such as those for fatty acids(1), terpenoids(2) and branched-chainamino acids(3). In spite of the importance of pyruvate uptake into plastids (organelles within cells of plants and algae), the molecular mechanisms of this uptake have not yet been explored. This is mainly because pyruvate is a relatively small compound that is able to passively permeate lipid bilayers(4), which precludes accurate measurement of pyruvate transport activity in reconstituted liposomes. Using differential transcriptome analyses of C(3) and C(4) plants of the genera Flaveria and Cleome, here we have identified a novel gene that is abundant in C(4) species, named BASS2 (BILE ACID:SODIUM SYMPORTER FAMILY PROTEIN 2). The BASS2 protein is localized at the chloroplast envelope membrane, and is highly abundant in C(4) plants that have the sodium-dependent pyruvate transporter. Recombinant BASS2 shows sodium-dependent pyruvate uptake activity. Sodium influx is balanced by a sodium: proton antiporter (NHD1), which was mimicked in recombinant Escherichia coli cells expressing both BASS2 and NHD1. Arabidopsis thaliana bass2 mutants lack pyruvate uptake into chloroplasts, which affects plastid-localized isopentenyl diphosphate synthesis, as evidenced by increased sensitivity of such mutants to mevastatin, an inhibitor of cytosolic isopentenyl diphosphate biosynthesis. We thus provide molecular evidence for a sodium-coupled metabolite transporter in plastid envelopes. Orthologues of BASS2 can be detected in all the genomes of land plants that have been characterized so far, thus indicating the widespread importance of sodium-coupled pyruvate import into plastids.
Publishing Year

Cite this

Furumoto T, Yamaguchi T, Ohshima-Ichie Y, et al. A plastidial sodium-dependent pyruvate transporter. Nature. 2011;476(7361):472-U131.
Furumoto, T., Yamaguchi, T., Ohshima-Ichie, Y., Nakamura, M., Tsuchida-Iwata, Y., Shimamura, M., Ohnishi, J., et al. (2011). A plastidial sodium-dependent pyruvate transporter. Nature, 476(7361), 472-U131. doi:10.1038/nature10250
Furumoto, T., Yamaguchi, T., Ohshima-Ichie, Y., Nakamura, M., Tsuchida-Iwata, Y., Shimamura, M., Ohnishi, J., Hata, S., Gowik, U., Westhoff, P., et al. (2011). A plastidial sodium-dependent pyruvate transporter. Nature 476, 472-U131.
Furumoto, T., et al., 2011. A plastidial sodium-dependent pyruvate transporter. Nature, 476(7361), p 472-U131.
T. Furumoto, et al., “A plastidial sodium-dependent pyruvate transporter”, Nature, vol. 476, 2011, pp. 472-U131.
Furumoto, T., Yamaguchi, T., Ohshima-Ichie, Y., Nakamura, M., Tsuchida-Iwata, Y., Shimamura, M., Ohnishi, J., Hata, S., Gowik, U., Westhoff, P., Bräutigam, A., Weber, A.P.M., Izui, K.: A plastidial sodium-dependent pyruvate transporter. Nature. 476, 472-U131 (2011).
Furumoto, Tsuyoshi, Yamaguchi, Teppei, Ohshima-Ichie, Yumiko, Nakamura, Masayoshi, Tsuchida-Iwata, Yoshiko, Shimamura, Masaki, Ohnishi, Junichi, Hata, Shingo, Gowik, Udo, Westhoff, Peter, Bräutigam, Andrea, Weber, Andreas P. M., and Izui, Katsura. “A plastidial sodium-dependent pyruvate transporter”. Nature 476.7361 (2011): 472-U131.
All files available under the following license(s):
Copyright Statement:
This Item is protected by copyright and/or related rights. [...]
Main File(s)
Access Level
OA Open Access
Last Uploaded

This data publication is cited in the following publications:
This publication cites the following data publications:

65 Citations in Europe PMC

Data provided by Europe PubMed Central.

A Non-photosynthetic Diatom Reveals Early Steps of Reductive Evolution in Plastids.
Kamikawa R, Moog D, Zauner S, Tanifuji G, Ishida KI, Miyashita H, Mayama S, Hashimoto T, Maier UG, Archibald JM, Inagaki Y., Mol. Biol. Evol. 34(9), 2017
PMID: 28549159
Chloroplast function and ion regulation in plants growing on saline soils: lessons from halophytes.
Bose J, Munns R, Shabala S, Gilliham M, Pogson B, Tyerman SD., J. Exp. Bot. 68(12), 2017
PMID: 28472512
Seed-Specific Overexpression of the Pyruvate Transporter BASS2 Increases Oil Content in Arabidopsis Seeds.
Lee EJ, Oh M, Hwang JU, Li-Beisson Y, Nishida I, Lee Y., Front Plant Sci 8(), 2017
PMID: 28265278
Metabolite pools and carbon flow during C4 photosynthesis in maize: 13CO2 labeling kinetics and cell type fractionation.
Arrivault S, Obata T, Szecowka M, Mengin V, Guenther M, Hoehne M, Fernie AR, Stitt M., J. Exp. Bot. 68(2), 2017
PMID: 27834209
Polyamine oxidase 5 loss-of-function mutations in Arabidopsis thaliana trigger metabolic and transcriptional reprogramming and promote salt stress tolerance.
Zarza X, Atanasov KE, Marco F, Arbona V, Carrasco P, Kopka J, Fotopoulos V, Munnik T, Gomez-Cadenas A, Tiburcio AF, Alcazar R., Plant Cell Environ. 40(4), 2017
PMID: 26791972
In silico metabolic network analysis of Arabidopsis leaves.
Beckers V, Dersch LM, Lotz K, Melzer G, Blasing OE, Fuchs R, Ehrhardt T, Wittmann C., BMC Syst Biol 10(1), 2016
PMID: 27793154
Fine-mapping and cross-validation of QTLs linked to fatty acid composition in multiple independent interspecific crosses of oil palm.
Ting NC, Yaakub Z, Kamaruddin K, Mayes S, Massawe F, Sambanthamurthi R, Jansen J, Low LE, Ithnin M, Kushairi A, Arulandoo X, Rosli R, Chan KL, Amiruddin N, Sritharan K, Lim CC, Nookiah R, Amiruddin MD, Singh R., BMC Genomics 17(), 2016
PMID: 27079197
Proteomic changes induced by potassium deficiency and potassium substitution by sodium in sugar beet.
Pi Z, Stevanato P, Sun F, Yang Y, Sun X, Zhao H, Geng G, Yu L., J. Plant Res. 129(3), 2016
PMID: 26860314
Biochemical approaches to C4 photosynthesis evolution studies: the case of malic enzymes decarboxylases.
Saigo M, Tronconi MA, Gerrard Wheeler MC, Alvarez CE, Drincovich MF, Andreo CS., Photosyn. Res. 117(1-3), 2013
PMID: 23832612


0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®


PMID: 21866161
PubMed | Europe PMC

Search this title in

Google Scholar