PLGG1, a plastidic glycolate glycerate transporter, is required for photorespiration and defines a unique class of metabolite transporters

Pick TR, Bräutigam A, Schulz MA, Obata T, Fernie AR, Weber APM (2013)
Proceedings of the National Academy of Sciences of the United States of America 110(8): 3185-3190.

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Photorespiratory carbon flux reaches up to a third of photosynthetic flux, thus contributes massively to the global carbon cycle. The pathway recycles glycolate-2-phosphate, the most abundant byproduct of RubisCO reactions. This oxygenation reaction of RubisCO and subsequent photorespiration significantly limit the biomass gains of many crop plants. Although photorespiration is a compartmentalized process with enzymatic reactions in the chloroplast, the peroxisomes, the mitochondria, and the cytosol, no transporter required for the core photorespiratory cycle has been identified at the molecular level to date. Using transcript coexpression analyses, we identified Plastidal glycolate glycerate translocator 1 (PLGG1) as a candidate core photorespiratory transporter. Related genes are encoded in the genomes of archaea, bacteria, fungi, and all Archaeplastida and have previously been associated with a function in programmed cell-death. A mutant deficient in PLGG1 shows WT-like growth only in an elevated carbon dioxide atmosphere. The mutant accumulates glycolate and glycerate, leading to the hypothesis that PLGG1 is a glycolate/glycerate transporter. This hypothesis was tested and supported by in vivo and in vitro transport assays and O-18(2)-metabolic flux profiling. Our results indicate that PLGG1 is the chloroplastidic glycolate/glycerate transporter, which is required for the function of the photorespiratory cycle. Identification of the PLGG1 transport function will facilitate unraveling the role of similar proteins in bacteria, archaea, and fungi in the future.
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Zeitschriftentitel
Proceedings of the National Academy of Sciences of the United States of America
Band
110
Zeitschriftennummer
8
Seite
3185-3190
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Pick TR, Bräutigam A, Schulz MA, Obata T, Fernie AR, Weber APM. PLGG1, a plastidic glycolate glycerate transporter, is required for photorespiration and defines a unique class of metabolite transporters. Proceedings of the National Academy of Sciences of the United States of America. 2013;110(8):3185-3190.
Pick, T. R., Bräutigam, A., Schulz, M. A., Obata, T., Fernie, A. R., & Weber, A. P. M. (2013). PLGG1, a plastidic glycolate glycerate transporter, is required for photorespiration and defines a unique class of metabolite transporters. Proceedings of the National Academy of Sciences of the United States of America, 110(8), 3185-3190. doi:10.1073/pnas.1215142110
Pick, T. R., Bräutigam, A., Schulz, M. A., Obata, T., Fernie, A. R., and Weber, A. P. M. (2013). PLGG1, a plastidic glycolate glycerate transporter, is required for photorespiration and defines a unique class of metabolite transporters. Proceedings of the National Academy of Sciences of the United States of America 110, 3185-3190.
Pick, T.R., et al., 2013. PLGG1, a plastidic glycolate glycerate transporter, is required for photorespiration and defines a unique class of metabolite transporters. Proceedings of the National Academy of Sciences of the United States of America, 110(8), p 3185-3190.
T.R. Pick, et al., “PLGG1, a plastidic glycolate glycerate transporter, is required for photorespiration and defines a unique class of metabolite transporters”, Proceedings of the National Academy of Sciences of the United States of America, vol. 110, 2013, pp. 3185-3190.
Pick, T.R., Bräutigam, A., Schulz, M.A., Obata, T., Fernie, A.R., Weber, A.P.M.: PLGG1, a plastidic glycolate glycerate transporter, is required for photorespiration and defines a unique class of metabolite transporters. Proceedings of the National Academy of Sciences of the United States of America. 110, 3185-3190 (2013).
Pick, Thea R., Bräutigam, Andrea, Schulz, Matthias A., Obata, Toshihiro, Fernie, Alisdair R., and Weber, Andreas P. M. “PLGG1, a plastidic glycolate glycerate transporter, is required for photorespiration and defines a unique class of metabolite transporters”. Proceedings of the National Academy of Sciences of the United States of America 110.8 (2013): 3185-3190.
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PMID: 26317137
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Daloso DM, Müller K, Obata T, Florian A, Tohge T, Bottcher A, Riondet C, Bariat L, Carrari F, Nunes-Nesi A, Buchanan BB, Reichheld JP, Araújo WL, Fernie AR., Proc Natl Acad Sci U S A 112(11), 2015
PMID: 25646482
Bending of protonema cells in a plastid glycolate/glycerate transporter knockout line of Physcomitrella patens.
Nakahara J, Takechi K, Myouga F, Moriyama Y, Sato H, Takio S, Takano H., PLoS One 10(3), 2015
PMID: 25793376
Metabolic connectivity as a driver of host and endosymbiont integration.
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PMID: 25825767
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PMID: 26008154
Suppression of glycolate oxidase causes glyoxylate accumulation that inhibits photosynthesis through deactivating Rubisco in rice.
Lu Y, Li Y, Yang Q, Zhang Z, Chen Y, Zhang S, Peng XX., Physiol Plant 150(3), 2014
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PMID: 24139444
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Kromdijk J, Ubierna N, Cousins AB, Griffiths H., J Exp Bot 65(13), 2014
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PMID: 24935935
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PMID: 24960279
Regulation of amino acid metabolic enzymes and transporters in plants.
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PMID: 25114014
Unknown components of the plastidial permeome.
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PMID: 25191333
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46 References

Daten bereitgestellt von Europe PubMed Central.

Inhibition of photosynthesis in Arabidopsis mutants lacking leaf glutamate synthase activity
Somerville CR, Ogren WL., 1980
D-GLYCERATE 3-KINASE, the last unknown enzyme in the photorespiratory cycle in Arabidopsis, belongs to a novel kinase family.
Boldt R, Edner C, Kolukisaoglu U, Hagemann M, Weckwerth W, Wienkoop S, Morgenthal K, Bauwe H., Plant Cell 17(8), 2005
PMID: 15980259
Identification of photorespiratory glutamate:glyoxylate aminotransferase (GGAT) gene in Arabidopsis.
Igarashi D, Miwa T, Seki M, Kobayashi M, Kato T, Tabata S, Shinozaki K, Ohsumi C., Plant J. 33(6), 2003
PMID: 12631323
Kinetic characteristics of the chloroplast envelope glycolate transporter
Howitz KT, McCarty RE., 1985
Substrate-specificity of the pea chloroplast glycolate transporter
Howitz KT, McCarty RE., 1985
Genome-wide insertional mutagenesis of Arabidopsis thaliana.
Alonso JM, Stepanova AN, Leisse TJ, Kim CJ, Chen H, Shinn P, Stevenson DK, Zimmerman J, Barajas P, Cheuk R, Gadrinab C, Heller C, Jeske A, Koesema E, Meyers CC, Parker H, Prednis L, Ansari Y, Choy N, Deen H, Geralt M, Hazari N, Hom E, Karnes M, Mulholland C, Ndubaku R, Schmidt I, Guzman P, Aguilar-Henonin L, Schmid M, Weigel D, Carter DE, Marchand T, Risseeuw E, Brogden D, Zeko A, Crosby WL, Berry CC, Ecker JR., Science 301(5633), 2003
PMID: 12893945
Tissue-specific expression of Arabidopsis AKT1 gene is consistent with a role in K+ nutrition.
Lagarde D, Basset M, Lepetit M, Conejero G, Gaymard F, Astruc S, Grignon C., Plant J. 9(2), 1996
PMID: 8820606
Metabolite profiling for plant functional genomics
Fiehn O., 2001
High quality metabolomic data for Chlamydomonas reinhardtii.
Lee DY, Fiehn O., Plant Methods 4(), 2008
PMID: 18442406
A gateway cloning vector set for high-throughput functional analysis of genes in planta.
Curtis MD, Grossniklaus U., Plant Physiol. 133(2), 2003
PMID: 14555774
The promoter of Tl-DNA gene 5 controls the tissue-specific expression of chimeric genes carried by a novel type of agrobacterium binary vector
Koncz C, Schell J., 1986
The Rx gene from potato controls separate virus resistance and cell death responses.
Bendahmane A, Kanyuka K, Baulcombe DC., Plant Cell 11(5), 1999
PMID: 10330465
A simple method for isolating import-competent Arabidopsis chloroplasts.
Aronsson H, Jarvis P., FEBS Lett. 529(2-3), 2002
PMID: 12372603

Bregman A., 1990

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