Azolla domestication towards a biobased economy?

Brouwer P, Bräutigam A, Külahoglu C, Tazelaar AOE, Kurz S, Nierop KGJ, van der Werf A, Weber APM, Schluepmann H (2014)
New Phytologist 202(3): 1069-1082.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
Due to its phenomenal growth requiring neither nitrogen fertilizer nor arable land and its biomass composition, the mosquito fern Azolla is a candidate crop to yield food, fuels and chemicals sustainably. To advance Azolla domestication, we research its dissemination, storage and transcriptome. Methods for dissemination, cross-fertilization and cryopreservation of the symbiosis Azolla filiculoides-Nostoc azollae are tested based on the fern spores. To study molecular processes in Azolla including spore induction, a database of 37649 unigenes from RNAseq of microsporocarps, megasporocarps and sporophytes was assembled, then validated. Spores obtained year-round germinated in vitro within 26d. In vitro fertilization rates reached 25%. Cryopreservation permitted storage for at least 7months. The unigene database entirely covered central metabolism and to a large degree covered cellular processes and regulatory networks. Analysis of genes engaged in transition to sexual reproduction revealed a FLOWERING LOCUS T-like protein in ferns with special features induced in sporulating Azolla fronds. Although domestication of a fern-cyanobacteria symbiosis may seem a daunting task, we conclude that the time is ripe and that results generated will serve to more widely access biochemicals in fern biomass for a biobased economy.
New Phytologist


Brouwer P, Bräutigam A, Külahoglu C, et al. Azolla domestication towards a biobased economy? New Phytologist. 2014;202(3):1069-1082.
Brouwer, P., Bräutigam, A., Külahoglu, C., Tazelaar, A. O. E., Kurz, S., Nierop, K. G. J., van der Werf, A., et al. (2014). Azolla domestication towards a biobased economy? New Phytologist, 202(3), 1069-1082. doi:10.1111/nph.12708
Brouwer, P., Bräutigam, A., Külahoglu, C., Tazelaar, A. O. E., Kurz, S., Nierop, K. G. J., van der Werf, A., Weber, A. P. M., and Schluepmann, H. (2014). Azolla domestication towards a biobased economy? New Phytologist 202, 1069-1082.
Brouwer, P., et al., 2014. Azolla domestication towards a biobased economy? New Phytologist, 202(3), p 1069-1082.
P. Brouwer, et al., “Azolla domestication towards a biobased economy?”, New Phytologist, vol. 202, 2014, pp. 1069-1082.
Brouwer, P., Bräutigam, A., Külahoglu, C., Tazelaar, A.O.E., Kurz, S., Nierop, K.G.J., van der Werf, A., Weber, A.P.M., Schluepmann, H.: Azolla domestication towards a biobased economy? New Phytologist. 202, 1069-1082 (2014).
Brouwer, Paul, Bräutigam, Andrea, Külahoglu, Canan, Tazelaar, Anne O. E., Kurz, Samantha, Nierop, Klaas G. J., van der Werf, Adrie, Weber, Andreas P. M., and Schluepmann, Henriette. “Azolla domestication towards a biobased economy?”. New Phytologist 202.3 (2014): 1069-1082.
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9 Zitationen in Europe PMC

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PMID: 29084347
Azolla along a phosphorus gradient: biphasic growth response linked to diazotroph traits and phosphorus-induced iron chlorosis.
Temmink RJM, Harpenslager SF, Smolders AJP, van Dijk G, Peters RCJH, Lamers LPM, van Kempen MML., Sci Rep 8(1), 2018
PMID: 29535346
Metabolic Adaptation, a Specialized Leaf Organ Structure and Vascular Responses to Diurnal N2 Fixation by Nostoc azollae Sustain the Astonishing Productivity of Azolla Ferns without Nitrogen Fertilizer.
Brouwer P, Bräutigam A, Buijs VA, Tazelaar AO, van der Werf A, Schlüter U, Reichart GJ, Bolger A, Usadel B, Weber AP, Schluepmann H., Front Plant Sci 8(), 2017
PMID: 28408911
Cytokinin-induced promotion of root meristem size in the fern Azolla supports a shoot-like origin of euphyllophyte roots.
de Vries J, de Vries J, Fischer AM, Roettger M, Rommel S, Schluepmann H, Bräutigam A, Carlsbecker A, Gould SB., New Phytol 209(2), 2016
PMID: 26358624
Field Guide to Plant Model Systems.
Chang C, Bowman JL, Meyerowitz EM., Cell 167(2), 2016
PMID: 27716506
Ferns: the missing link in shoot evolution and development.
Plackett AR, Di Stilio VS, Langdale JA., Front Plant Sci 6(), 2015
PMID: 26594222
Between two fern genomes.
Sessa EB, Banks JA, Barker MS, Der JP, Duffy AM, Graham SW, Hasebe M, Langdale J, Li FW, Marchant DB, Pryer KM, Rothfels CJ, Roux SJ, Salmi ML, Sigel EM, Soltis DE, Soltis PS, Stevenson DW, Wolf PG., Gigascience 3(), 2014
PMID: 25324969

63 References

Daten bereitgestellt von Europe PubMed Central.

RNA-Seq Assembly - Are We There Yet?
Schliesky S, Gowik U, Weber AP, Brautigam A., Front Plant Sci 3(), 2012
PMID: 23056003

Shuying, 1987
Emissions of N2O and NO associated with nitrogen fertilization in intensive agriculture, and the potential for mitigation
Smith, Soil Use and Management 13(), 1997
Regulation of flowering time: all roads lead to Rome.
Srikanth A, Schmid M., Cell. Mol. Life Sci. 68(12), 2011
PMID: 21611891
Sporocarps of Azolla mexicana Presl. I. isolation and purification of megasporocarps and microsporangia
Toia, New Phytologist 106(), 1987
Approximation properties of haplotype tagging.
Vinterbo SA, Dreiseitl S, Ohno-Machado L., BMC Bioinformatics 7(), 2006
PMID: 16401341
A guide to using MapMan to visualize and compare Omics data in plants: a case study in the crop species, Maize.
Usadel B, Poree F, Nagel A, Lohse M, Czedik-Eysenberg A, Stitt M., Plant Cell Environ. 32(9), 2009
PMID: 19389052
Azolla: a review of its biology and utilization
Wagner, Botanical Review 63(), 1997

Watanabe, 1992
Evolution of plant reproduction: from fusion and dispersal to interaction and communication
Willemse, Chinese Science Bulletin 54(), 2009

Cellular responses in the cyanobacterial symbiont during its vertical transfer between plant generations in the Azolla microphylla symbiosis.
Zheng W, Bergman B, Chen B, Zheng S, Guan X, Xiang G, Rasmussen U., New Phytol. 181(1), 2009
PMID: 19076717


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