Comparative Transcriptome Atlases Reveal Altered Gene Expression Modules between Two Cleomaceae C-3 and C-4 Plant Species

Külahoglu C, Denton AK, Sommer M, Mass J, Schliesky S, Wrobel TJ, Berckmans B, Gongora-Castillo E, Buell CR, Simon R, De Veylder L, et al. (2014)
Plant Cell 26(8): 3243-3260.

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C-4 photosynthesis outperforms the ancestral C-3 state in a wide range of natural and agro-ecosystems by affording higher water-use and nitrogen-use efficiencies. It therefore represents a prime target for engineering novel, high-yielding crops by introducing the trait into C-3 backgrounds. However, the genetic architecture of C-4 photosynthesis remains largely unknown. To define the divergence in gene expression modules between C-3 and C-4 photosynthesis during leaf ontogeny, we generated comprehensive transcriptome atlases of two Cleomaceae species, Gynandropsis gynandra (C-4) and Tarenaya hassleriana (C-3), by RNA sequencing. Overall, the gene expression profiles appear remarkably similar between the C-3 and C-4 species. We found that known C-4 genes were recruited to photosynthesis from different expression domains in C-3, including typical housekeeping gene expression patterns in various tissues as well as individual heterotrophic tissues. Furthermore, we identified a structure-related module recruited from the C-3 root. Comparison of gene expression patterns with anatomy during leaf ontogeny provided insight into genetic features of Kranz anatomy. Altered expression of developmental factors and cell cycle genes is associated with a higher degree of endoreduplication in enlarged C-4 bundle sheath cells. A delay in mesophyll differentiation apparent both in the leaf anatomy and the transcriptome allows for extended vein formation in the C-4 leaf.
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Külahoglu C, Denton AK, Sommer M, et al. Comparative Transcriptome Atlases Reveal Altered Gene Expression Modules between Two Cleomaceae C-3 and C-4 Plant Species. Plant Cell. 2014;26(8):3243-3260.
Külahoglu, C., Denton, A. K., Sommer, M., Mass, J., Schliesky, S., Wrobel, T. J., Berckmans, B., et al. (2014). Comparative Transcriptome Atlases Reveal Altered Gene Expression Modules between Two Cleomaceae C-3 and C-4 Plant Species. Plant Cell, 26(8), 3243-3260. doi:10.1105/tpc.114.123752
Külahoglu, C., Denton, A. K., Sommer, M., Mass, J., Schliesky, S., Wrobel, T. J., Berckmans, B., Gongora-Castillo, E., Buell, C. R., Simon, R., et al. (2014). Comparative Transcriptome Atlases Reveal Altered Gene Expression Modules between Two Cleomaceae C-3 and C-4 Plant Species. Plant Cell 26, 3243-3260.
Külahoglu, C., et al., 2014. Comparative Transcriptome Atlases Reveal Altered Gene Expression Modules between Two Cleomaceae C-3 and C-4 Plant Species. Plant Cell, 26(8), p 3243-3260.
C. Külahoglu, et al., “Comparative Transcriptome Atlases Reveal Altered Gene Expression Modules between Two Cleomaceae C-3 and C-4 Plant Species”, Plant Cell, vol. 26, 2014, pp. 3243-3260.
Külahoglu, C., Denton, A.K., Sommer, M., Mass, J., Schliesky, S., Wrobel, T.J., Berckmans, B., Gongora-Castillo, E., Buell, C.R., Simon, R., De Veylder, L., Bräutigam, A., Weber, A.P.M.: Comparative Transcriptome Atlases Reveal Altered Gene Expression Modules between Two Cleomaceae C-3 and C-4 Plant Species. Plant Cell. 26, 3243-3260 (2014).
Külahoglu, Canan, Denton, Alisandra K., Sommer, Manuel, Mass, Janina, Schliesky, Simon, Wrobel, Thomas J., Berckmans, Barbara, Gongora-Castillo, Elsa, Buell, C. Robin, Simon, Ruediger, De Veylder, Lieven, Bräutigam, Andrea, and Weber, Andreas P. M. “Comparative Transcriptome Atlases Reveal Altered Gene Expression Modules between Two Cleomaceae C-3 and C-4 Plant Species”. Plant Cell 26.8 (2014): 3243-3260.
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