Cytokinin-induced promotion of root meristem size in the fern Azolla supports a shoot-like origin of euphyllophyte roots

de Vries J, Fischer AM, Roettger M, Rommel S, Schluepmann H, Bräutigam A, Carlsbecker A, Gould SB (2016)
New Phytologist 209(2): 705-720.

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Abstract
The phytohormones cytokinin and auxin orchestrate the root meristem development in angiosperms by determining embryonic bipolarity. Ferns, having the most basal euphyllophyte root, form neither bipolar embryos nor permanent embryonic primary roots but rather an adventitious root system. This raises the questions of how auxin and cytokinin govern fern root system architecture and whether this can tell us something about the origin of that root. Using Azolla filiculoides, we characterized the influence of IAA and zeatin on adventitious fern root meristems and vasculature by Nomarski microscopy. Simultaneously, RNAseq analyses, yielding 36 091 contigs, were used to uncover how the phytohormones affect root tip gene expression. We show that auxin restricts Azolla root meristem development, while cytokinin promotes it; it is the opposite effect of what is observed in Arabidopsis. Global gene expression profiling uncovered 145 genes significantly regulated by cytokinin or auxin, including cell wall modulators, cell division regulators and lateral root formation coordinators. Our data illuminate both evolution and development of fern roots. Promotion of meristem size through cytokinin supports the idea that root meristems of euphyllophytes evolved from shoot meristems. The foundation of these roots was laid in a postembryonically branching shoot system.
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de Vries J, Fischer AM, Roettger M, et al. Cytokinin-induced promotion of root meristem size in the fern Azolla supports a shoot-like origin of euphyllophyte roots. New Phytologist. 2016;209(2):705-720.
de Vries, J., Fischer, A. M., Roettger, M., Rommel, S., Schluepmann, H., Bräutigam, A., Carlsbecker, A., et al. (2016). Cytokinin-induced promotion of root meristem size in the fern Azolla supports a shoot-like origin of euphyllophyte roots. New Phytologist, 209(2), 705-720. doi:10.1111/nph.13630
de Vries, J., Fischer, A. M., Roettger, M., Rommel, S., Schluepmann, H., Bräutigam, A., Carlsbecker, A., and Gould, S. B. (2016). Cytokinin-induced promotion of root meristem size in the fern Azolla supports a shoot-like origin of euphyllophyte roots. New Phytologist 209, 705-720.
de Vries, J., et al., 2016. Cytokinin-induced promotion of root meristem size in the fern Azolla supports a shoot-like origin of euphyllophyte roots. New Phytologist, 209(2), p 705-720.
J. de Vries, et al., “Cytokinin-induced promotion of root meristem size in the fern Azolla supports a shoot-like origin of euphyllophyte roots”, New Phytologist, vol. 209, 2016, pp. 705-720.
de Vries, J., Fischer, A.M., Roettger, M., Rommel, S., Schluepmann, H., Bräutigam, A., Carlsbecker, A., Gould, S.B.: Cytokinin-induced promotion of root meristem size in the fern Azolla supports a shoot-like origin of euphyllophyte roots. New Phytologist. 209, 705-720 (2016).
de Vries, Jan, Fischer, Angela Melanie, Roettger, Mayo, Rommel, Sophie, Schluepmann, Henriette, Bräutigam, Andrea, Carlsbecker, Annelie, and Gould, Sven Bernhard. “Cytokinin-induced promotion of root meristem size in the fern Azolla supports a shoot-like origin of euphyllophyte roots”. New Phytologist 209.2 (2016): 705-720.
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PMID: 27994131
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129 References

Data provided by Europe PubMed Central.

Phylotranscriptomic analysis oft he origin and early diversification of land plants
AUTHOR UNKNOWN, 2014
An "Electronic Fluorescent Pictograph" browser for exploring and analyzing large-scale biological data sets.
Winter D, Vinegar B, Nahal H, Ammar R, Wilson GV, Provart NJ., PLoS ONE 2(8), 2007
PMID: 17684564
Origin of land plants: do conjugating green algae hold the key?
Wodniok S, Brinkmann H, Glockner G, Heidel AJ, Philippe H, Melkonian M, Becker B., BMC Evol. Biol. 11(), 2011
PMID: 21501468

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