Isolation and whole genome analysis of endospore-forming bacteria from heroin.

Kalinowski J, Ahrens B, Al-Dilaimi A, Winkler A, Wibberg D, Schleenbecker U, Rückert C, Wölfel R, Grass G (2017)
Forensic Sci Int Genet 32: 1-6.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Abstract / Bemerkung
Infections caused by endospore-forming bacteria have been associated with severe illness and death among persons who inject drugs. Analysis of the bacteria residing in heroin has thus been biased towards species that affect human health. Similarly, exploration of the bacterial diversity of seized street market heroin correlated with the skin microflora of recreational heroin users insofar as different Staphylococus spp. or typical environmental endospore formers including Bacillus cereus and other Bacilli outside the B. cereus sensu lato group as well as diverse Clostridia were identified. In this work 82 samples of non-street market ("wholesale") heroin originating from the German Federal Criminal Police Office's heroin analysis program seized during the period between 2009 and 2014 were analyzed for contaminating bacteria. Without contact with the end user and with only little contaminations introduced by final processing, adulteration and cutting this heroin likely harbors original microbiota from the drug's original source or trafficking route. We found this drug to be only sparsely populated with retrievable heterotrophic, aerobic bacteria. In total, 68 isolates were retrieved from 49 out of 82 samples analyzed (60% culture positive). All isolates were endospore-forming, Gram-positive Bacilli. Completely absent were non-endospore-formers or Gram-negatives. The three most predominant species were Bacillus clausii, Bacillus (para)licheniformis, and Terribacillus saccharophilus. Whole genome sequencing of these 68 isolates was performed using Illumina technology. Sequence data sets were assembled and annotated using an automated bioinformatics pipeline. Average nucleotide identity (ANI) values were calculated for all draft genomes and all close to identical genomes (ANI>99.5%) were compared to the forensic data of the seized drug, showing positive correlations that strongly warrant further research on this subject.
Erscheinungsjahr
Zeitschriftentitel
Forensic Sci Int Genet
Band
32
Seite
1-6
ISSN
eISSN
PUB-ID

Zitieren

Kalinowski J, Ahrens B, Al-Dilaimi A, et al. Isolation and whole genome analysis of endospore-forming bacteria from heroin. Forensic Sci Int Genet. 2017;32:1-6.
Kalinowski, J., Ahrens, B., Al-Dilaimi, A., Winkler, A., Wibberg, D., Schleenbecker, U., Rückert, C., et al. (2017). Isolation and whole genome analysis of endospore-forming bacteria from heroin. Forensic Sci Int Genet, 32, 1-6. doi:10.1016/j.fsigen.2017.10.001
Kalinowski, J., Ahrens, B., Al-Dilaimi, A., Winkler, A., Wibberg, D., Schleenbecker, U., Rückert, C., Wölfel, R., and Grass, G. (2017). Isolation and whole genome analysis of endospore-forming bacteria from heroin. Forensic Sci Int Genet 32, 1-6.
Kalinowski, J., et al., 2017. Isolation and whole genome analysis of endospore-forming bacteria from heroin. Forensic Sci Int Genet, 32, p 1-6.
J. Kalinowski, et al., “Isolation and whole genome analysis of endospore-forming bacteria from heroin.”, Forensic Sci Int Genet, vol. 32, 2017, pp. 1-6.
Kalinowski, J., Ahrens, B., Al-Dilaimi, A., Winkler, A., Wibberg, D., Schleenbecker, U., Rückert, C., Wölfel, R., Grass, G.: Isolation and whole genome analysis of endospore-forming bacteria from heroin. Forensic Sci Int Genet. 32, 1-6 (2017).
Kalinowski, Jörn, Ahrens, B, Al-Dilaimi, Arwa, Winkler, Anika, Wibberg, Daniel, Schleenbecker, U, Rückert, Christian, Wölfel, R, and Grass, G. “Isolation and whole genome analysis of endospore-forming bacteria from heroin.”. Forensic Sci Int Genet 32 (2017): 1-6.

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 29024922
PubMed | Europe PMC

Suchen in

Google Scholar