Biophysical characterization of the association of histones with single-stranded DNA.

Wang Y, van Merwyk L, Tönsing K, Walhorn V, Anselmetti D, Fernàndez-Busquets X (2017)
Biochimica et Biophysica Acta 1861(11, Pt. A): 2739-2749.

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Abstract
BACKGROUND:
Despite the profound current knowledge of the architecture and dynamics of nucleosomes, little is known about the structures generated by the interaction of histones with single-stranded DNA (ssDNA), which is widely present during replication and transcription.
METHODS:
Non-denaturing gel electrophoresis, transmission electron microscopy, atomic force microscopy, magnetic tweezers.
RESULTS:
Histones have a high affinity for ssDNA in 0.15M NaCl ionic strength, with an apparent binding constant similar to that calculated for their association with double-stranded DNA (dsDNA). The length of DNA (number of nucleotides in ssDNA or base pairs in dsDNA) associated with a fixed core histone mass is the same for both ssDNA and dsDNA. Although histone-ssDNA complexes show a high tendency to aggregate, nucleosome-like structures are formed at physiological salt concentrations. Core histones are able to protect ssDNA from digestion by micrococcal nuclease, and a shortening of ssDNA occurs upon its interaction with histones. The purified (+) strand of a cloned DNA fragment of nucleosomal origin has a higher affinity for histones than the purified complementary (-) strand.
CONCLUSIONS:
At physiological ionic strength histones have high affinity for ssDNA, possibly associating with it into nucleosome-like structures.
GENERAL SIGNIFICANCE:
In the cell nucleus histones may spontaneously interact with ssDNA to facilitate their participation in the replication and transcription of chromatin.
Copyright © 2017 Elsevier B.V. All rights reserved.
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Wang Y, van Merwyk L, Tönsing K, Walhorn V, Anselmetti D, Fernàndez-Busquets X. Biophysical characterization of the association of histones with single-stranded DNA. Biochimica et Biophysica Acta. 2017;1861(11, Pt. A):2739-2749.
Wang, Y., van Merwyk, L., Tönsing, K., Walhorn, V., Anselmetti, D., & Fernàndez-Busquets, X. (2017). Biophysical characterization of the association of histones with single-stranded DNA. Biochimica et Biophysica Acta, 1861(11, Pt. A), 2739-2749. doi:10.1016/j.bbagen.2017.07.018
Wang, Y., van Merwyk, L., Tönsing, K., Walhorn, V., Anselmetti, D., and Fernàndez-Busquets, X. (2017). Biophysical characterization of the association of histones with single-stranded DNA. Biochimica et Biophysica Acta 1861, 2739-2749.
Wang, Y., et al., 2017. Biophysical characterization of the association of histones with single-stranded DNA. Biochimica et Biophysica Acta, 1861(11, Pt. A), p 2739-2749.
Y. Wang, et al., “Biophysical characterization of the association of histones with single-stranded DNA.”, Biochimica et Biophysica Acta, vol. 1861, 2017, pp. 2739-2749.
Wang, Y., van Merwyk, L., Tönsing, K., Walhorn, V., Anselmetti, D., Fernàndez-Busquets, X.: Biophysical characterization of the association of histones with single-stranded DNA. Biochimica et Biophysica Acta. 1861, 2739-2749 (2017).
Wang, Ying, van Merwyk, Luis, Tönsing, Katja, Walhorn, Volker, Anselmetti, Dario, and Fernàndez-Busquets, Xavier. “Biophysical characterization of the association of histones with single-stranded DNA.”. Biochimica et Biophysica Acta 1861.11, Pt. A (2017): 2739-2749.
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