Biophysical characterization of the association of histones with single-stranded DNA.

Wang Y, van Merwyk L, Tönsing K, Walhorn V, Anselmetti D, Fernàndez-Busquets X (2017)
Biochimica et Biophysica Acta 1861(11, Pt. A): 2739-2749.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Elektronische Veröffentlichung vor dem Druck | Englisch
Autor
; ; ; ; ;
Abstract / Bemerkung
BACKGROUND:
Despite the profound current knowledge of the architecture and dynamics of nucleosomes, little is known about the structures generated by the interaction of histones with single-stranded DNA (ssDNA), which is widely present during replication and transcription.
METHODS:
Non-denaturing gel electrophoresis, transmission electron microscopy, atomic force microscopy, magnetic tweezers.
RESULTS:
Histones have a high affinity for ssDNA in 0.15M NaCl ionic strength, with an apparent binding constant similar to that calculated for their association with double-stranded DNA (dsDNA). The length of DNA (number of nucleotides in ssDNA or base pairs in dsDNA) associated with a fixed core histone mass is the same for both ssDNA and dsDNA. Although histone-ssDNA complexes show a high tendency to aggregate, nucleosome-like structures are formed at physiological salt concentrations. Core histones are able to protect ssDNA from digestion by micrococcal nuclease, and a shortening of ssDNA occurs upon its interaction with histones. The purified (+) strand of a cloned DNA fragment of nucleosomal origin has a higher affinity for histones than the purified complementary (-) strand.
CONCLUSIONS:
At physiological ionic strength histones have high affinity for ssDNA, possibly associating with it into nucleosome-like structures.
GENERAL SIGNIFICANCE:
In the cell nucleus histones may spontaneously interact with ssDNA to facilitate their participation in the replication and transcription of chromatin.
Copyright © 2017 Elsevier B.V. All rights reserved.
Erscheinungsjahr
Zeitschriftentitel
Biochimica et Biophysica Acta
Band
1861
Zeitschriftennummer
11, Pt. A
Seite
2739-2749
ISSN
PUB-ID

Zitieren

Wang Y, van Merwyk L, Tönsing K, Walhorn V, Anselmetti D, Fernàndez-Busquets X. Biophysical characterization of the association of histones with single-stranded DNA. Biochimica et Biophysica Acta. 2017;1861(11, Pt. A):2739-2749.
Wang, Y., van Merwyk, L., Tönsing, K., Walhorn, V., Anselmetti, D., & Fernàndez-Busquets, X. (2017). Biophysical characterization of the association of histones with single-stranded DNA. Biochimica et Biophysica Acta, 1861(11, Pt. A), 2739-2749. doi:10.1016/j.bbagen.2017.07.018
Wang, Y., van Merwyk, L., Tönsing, K., Walhorn, V., Anselmetti, D., and Fernàndez-Busquets, X. (2017). Biophysical characterization of the association of histones with single-stranded DNA. Biochimica et Biophysica Acta 1861, 2739-2749.
Wang, Y., et al., 2017. Biophysical characterization of the association of histones with single-stranded DNA. Biochimica et Biophysica Acta, 1861(11, Pt. A), p 2739-2749.
Y. Wang, et al., “Biophysical characterization of the association of histones with single-stranded DNA.”, Biochimica et Biophysica Acta, vol. 1861, 2017, pp. 2739-2749.
Wang, Y., van Merwyk, L., Tönsing, K., Walhorn, V., Anselmetti, D., Fernàndez-Busquets, X.: Biophysical characterization of the association of histones with single-stranded DNA. Biochimica et Biophysica Acta. 1861, 2739-2749 (2017).
Wang, Ying, van Merwyk, Luis, Tönsing, Katja, Walhorn, Volker, Anselmetti, Dario, and Fernàndez-Busquets, Xavier. “Biophysical characterization of the association of histones with single-stranded DNA.”. Biochimica et Biophysica Acta 1861.11, Pt. A (2017): 2739-2749.

2 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Binding mechanism of anti-cancer chemotherapeutic drug mitoxantrone to DNA characterized by magnetic tweezers.
Kreft D, Wang Y, Rattay M, Toensing K, Anselmetti D., J Nanobiotechnology 16(1), 2018
PMID: 30005668
Bre1-dependent H2B ubiquitination promotes homologous recombination by stimulating histone eviction at DNA breaks.
Zheng S, Li D, Lu Z, Liu G, Wang M, Xing P, Wang M, Dong Y, Wang X, Li J, Zhang S, Peng H, Ira G, Li G, Chen X., Nucleic Acids Res (), 2018
PMID: 30304473

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 28756274
PubMed | Europe PMC

Suchen in

Google Scholar