Synthesis of Chiral Cyanohydrins by Recombinant Escherichia coli Cells in a Micro-Aqueous Reaction System

Scholz KE, Okrob D, Kopka B, Grünberger A, Pohl M, Jaeger K-E, Krauss U (2012)
Applied and Environmental Microbiology 78(14): 5025-5027.

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Synthesis of chiral cyanohydrins is performed in a monophasic micro-aqueous reaction system using whole recombinant Escherichia coli cells expressing the Arabidopsis thaliana hydroxynitrile lyase (AtHNL). Microscopy studies employing a fusion of AtHNL with a flavin-based fluorescent protein (FbFP) reveal that the cells remain intact in the reaction system.
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Scholz KE, Okrob D, Kopka B, et al. Synthesis of Chiral Cyanohydrins by Recombinant Escherichia coli Cells in a Micro-Aqueous Reaction System. Applied and Environmental Microbiology. 2012;78(14):5025-5027.
Scholz, K. E., Okrob, D., Kopka, B., Grünberger, A., Pohl, M., Jaeger, K. - E., & Krauss, U. (2012). Synthesis of Chiral Cyanohydrins by Recombinant Escherichia coli Cells in a Micro-Aqueous Reaction System. Applied and Environmental Microbiology, 78(14), 5025-5027. doi:10.1128/aem.00582-12
Scholz, K. E., Okrob, D., Kopka, B., Grünberger, A., Pohl, M., Jaeger, K. - E., and Krauss, U. (2012). Synthesis of Chiral Cyanohydrins by Recombinant Escherichia coli Cells in a Micro-Aqueous Reaction System. Applied and Environmental Microbiology 78, 5025-5027.
Scholz, K.E., et al., 2012. Synthesis of Chiral Cyanohydrins by Recombinant Escherichia coli Cells in a Micro-Aqueous Reaction System. Applied and Environmental Microbiology, 78(14), p 5025-5027.
K.E. Scholz, et al., “Synthesis of Chiral Cyanohydrins by Recombinant Escherichia coli Cells in a Micro-Aqueous Reaction System”, Applied and Environmental Microbiology, vol. 78, 2012, pp. 5025-5027.
Scholz, K.E., Okrob, D., Kopka, B., Grünberger, A., Pohl, M., Jaeger, K.-E., Krauss, U.: Synthesis of Chiral Cyanohydrins by Recombinant Escherichia coli Cells in a Micro-Aqueous Reaction System. Applied and Environmental Microbiology. 78, 5025-5027 (2012).
Scholz, Kathrin Emmi, Okrob, Daniel, Kopka, Benita, Grünberger, Alexander, Pohl, Martina, Jaeger, Karl-Erich, and Krauss, Ulrich. “Synthesis of Chiral Cyanohydrins by Recombinant Escherichia coli Cells in a Micro-Aqueous Reaction System”. Applied and Environmental Microbiology 78.14 (2012): 5025-5027.
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