Single-cell microfluidics: opportunity for bioprocess development

Grünberger A, Wiechert W, Kohlheyer D (2014)
Current opinion in biotechnology 29: 15-23.

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Cell-to-cell heterogeneity in microbial biotechnological processes caused by biological (intrinsic) and environmental (extrinsic) fluctuations can have a severe impact on productivity. However, as yet little is known about the complex interplay between environmental reactor dynamics and cellular activity. A few years ago, innovative microfluidic systems were introduced facilitating the spatiotemporal analysis of single cells under well-defined environmental conditions allowing so far unachievable insights into population heterogeneity and bioreactor inhomogeneity. Examples of microfabricated systems include microfluidic cavities harbouring micropopulations of several thousand cells down to femtolitre-size structures entrapping individual bacteria. In well-defined perfusion experiments, central questions in biotechnology regarding, for example, growth, productivity, and heterogeneity on the single-cell level have been addressed for the first time. Microfluidics will take its place as a single-cell analytical technique in biotechnological process and strain characterization.
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Grünberger A, Wiechert W, Kohlheyer D. Single-cell microfluidics: opportunity for bioprocess development. Current opinion in biotechnology. 2014;29:15-23.
Grünberger, A., Wiechert, W., & Kohlheyer, D. (2014). Single-cell microfluidics: opportunity for bioprocess development. Current opinion in biotechnology, 29, 15-23. doi:10.1016/j.copbio.2014.02.008
Grünberger, A., Wiechert, W., and Kohlheyer, D. (2014). Single-cell microfluidics: opportunity for bioprocess development. Current opinion in biotechnology 29, 15-23.
Grünberger, A., Wiechert, W., & Kohlheyer, D., 2014. Single-cell microfluidics: opportunity for bioprocess development. Current opinion in biotechnology, 29, p 15-23.
A. Grünberger, W. Wiechert, and D. Kohlheyer, “Single-cell microfluidics: opportunity for bioprocess development”, Current opinion in biotechnology, vol. 29, 2014, pp. 15-23.
Grünberger, A., Wiechert, W., Kohlheyer, D.: Single-cell microfluidics: opportunity for bioprocess development. Current opinion in biotechnology. 29, 15-23 (2014).
Grünberger, Alexander, Wiechert, Wolfgang, and Kohlheyer, Dietrich. “Single-cell microfluidics: opportunity for bioprocess development”. Current opinion in biotechnology 29 (2014): 15-23.
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