Technical bias of microcultivation environments on single cell physiology

Dusny C, Grünberger A, Probst C, Wiechert W, Kohlheyer D, Schmid A (2015)
Lab on a chip 15(8): 1822-1834.

Download
No fulltext has been uploaded. References only!
Journal Article | Original Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ;
Abstract
Microscale cultivation systems are important tools to elucidate cellular dynamics beyond the population average and understand the functional architecture of single cells. However, there is scant knowledge about the bias of different microcultivation technologies on cellular functions. We therefore performed a systematic cross-platform comparison of three different microscale cultivation systems commonly harnessed in single-cell analysis: microfluidic non-contact cell traps driven by negative dielectrophoresis, microfluidic monolayer growth chambers, and semi-solid agarose pads. We assessed the specific single-cell growth rates, division rates and morphological characteristics of single Corynebacterium glutamicum cells and microcolonies as a bacterial model organism with medical and biotechnological relevance under standardized growth conditions. Strikingly, the specific single-cell and microcolony growth rates, μmax, were robust and conserved for several cell generations with all three microcultivation technologies, whereas the division rates of cells grown on agarose pads deviated by up to 50% from those of cells cultivated in negative dielectrophoresis traps and monolayer growth chambers. Furthermore, morphological characteristics like cell lengths and division symmetries of individual cells were affected when the cells were grown on agarose pads. This indicated a significant impact of solid cultivation supports on cellular traits. The results demonstrate the impact of microcultivation technology on microbial physiology for the first time and show the need for a careful selection and design of the microcultivation technology in order to allow unbiased analysis of cellular behavior.
Publishing Year
eISSN
PUB-ID

Cite this

Dusny C, Grünberger A, Probst C, Wiechert W, Kohlheyer D, Schmid A. Technical bias of microcultivation environments on single cell physiology. Lab on a chip. 2015;15(8):1822-1834.
Dusny, C., Grünberger, A., Probst, C., Wiechert, W., Kohlheyer, D., & Schmid, A. (2015). Technical bias of microcultivation environments on single cell physiology. Lab on a chip, 15(8), 1822-1834. doi:10.1039/C4LC01270D
Dusny, C., Grünberger, A., Probst, C., Wiechert, W., Kohlheyer, D., and Schmid, A. (2015). Technical bias of microcultivation environments on single cell physiology. Lab on a chip 15, 1822-1834.
Dusny, C., et al., 2015. Technical bias of microcultivation environments on single cell physiology. Lab on a chip, 15(8), p 1822-1834.
C. Dusny, et al., “Technical bias of microcultivation environments on single cell physiology”, Lab on a chip, vol. 15, 2015, pp. 1822-1834.
Dusny, C., Grünberger, A., Probst, C., Wiechert, W., Kohlheyer, D., Schmid, A.: Technical bias of microcultivation environments on single cell physiology. Lab on a chip. 15, 1822-1834 (2015).
Dusny, Christian, Grünberger, Alexander, Probst, Christopher, Wiechert, Wolfgang, Kohlheyer, Dietrich, and Schmid, Andreas. “Technical bias of microcultivation environments on single cell physiology”. Lab on a chip 15.8 (2015): 1822-1834.
This data publication is cited in the following publications:
This publication cites the following data publications:

10 Citations in Europe PMC

Data provided by Europe PubMed Central.

Recent Advances in the Analysis of Single Cells.
Armbrecht L, Dittrich PS., Anal Chem 89(1), 2017
PMID: 28105840
Coarse-graining bacteria colonies for modelling critical solute distributions in picolitre bioreactors for bacterial studies on single-cell level.
Westerwalbesloh C, Grünberger A, Wiechert W, Kohlheyer D, von Lieres E., Microb Biotechnol 10(4), 2017
PMID: 28371389
Homogenizing bacterial cell factories: Analysis and engineering of phenotypic heterogeneity.
Binder D, Drepper T, Jaeger KE, Delvigne F, Wiechert W, Kohlheyer D, Grünberger A., Metab Eng 42(), 2017
PMID: 28645641
Beyond the bulk: disclosing the life of single microbial cells.
Rosenthal K, Oehling V, Dusny C, Schmid A., FEMS Microbiol Rev 41(6), 2017
PMID: 29029257
Comparative Single-Cell Analysis of Different E. coli Expression Systems during Microfluidic Cultivation.
Binder D, Probst C, Grünberger A, Hilgers F, Loeschcke A, Jaeger KE, Kohlheyer D, Drepper T., PLoS One 11(8), 2016
PMID: 27525986
Modeling and CFD simulation of nutrient distribution in picoliter bioreactors for bacterial growth studies on single-cell level.
Westerwalbesloh C, Grünberger A, Stute B, Weber S, Wiechert W, Kohlheyer D, von Lieres E., Lab Chip 15(21), 2015
PMID: 26345659
Spatiotemporal microbial single-cell analysis using a high-throughput microfluidics cultivation platform.
Grünberger A, Probst C, Helfrich S, Nanda A, Stute B, Wiechert W, von Lieres E, Nöh K, Frunzke J, Kohlheyer D., Cytometry A 87(12), 2015
PMID: 26348020

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 25710324
PubMed | Europe PMC

Search this title in

Google Scholar