Photocaged Arabinose - A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression

Binder D, Bier C, Grünberger A, Drobietz D, Hage-Hülsmann J, rey G, Büchs J, Kohlheyer D, Loeschcke A, Wiechert W, Jaeger K-E, et al. (2016)
ChemBioChem 17(4): 296-299.

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Controlling cellular functions by light allows simple triggering of biological processes in a non-invasive fashion with high spatiotemporal resolution. In this context, light-regulated gene expression has enormous potential for achieving optogenetic control over almost any cellular process. Here, we report on two novel one-step cleavable photocaged arabinose compounds, which were applied as light-sensitive inducers of transcription in bacteria. Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production, as demonstrated for GFP and the complete violacein biosynthetic pathway. Moreover, single-cell analysis revealed that intrinsic heterogeneity of arabinose-mediated induction of gene expression was overcome when using photocaged arabinose. We have thus established a novel phototrigger for synthetic bio(techno)logy applications that enables precise and homogeneous control of bacterial target gene expression.
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Binder D, Bier C, Grünberger A, et al. Photocaged Arabinose - A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression. ChemBioChem. 2016;17(4):296-299.
Binder, D., Bier, C., Grünberger, A., Drobietz, D., Hage-Hülsmann, J., rey, G., Büchs, J., et al. (2016). Photocaged Arabinose - A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression. ChemBioChem, 17(4), 296-299. doi:10.1002/cbic.201500609
Binder, D., Bier, C., Grünberger, A., Drobietz, D., Hage-Hülsmann, J., rey, G., Büchs, J., Kohlheyer, D., Loeschcke, A., Wiechert, W., et al. (2016). Photocaged Arabinose - A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression. ChemBioChem 17, 296-299.
Binder, D., et al., 2016. Photocaged Arabinose - A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression. ChemBioChem, 17(4), p 296-299.
D. Binder, et al., “Photocaged Arabinose - A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression”, ChemBioChem, vol. 17, 2016, pp. 296-299.
Binder, D., Bier, C., Grünberger, A., Drobietz, D., Hage-Hülsmann, J., rey, G., Büchs, J., Kohlheyer, D., Loeschcke, A., Wiechert, W., Jaeger, K.-E., Pietruszka, J., Drepper, T.: Photocaged Arabinose - A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression. ChemBioChem. 17, 296-299 (2016).
Binder, Dennis, Bier, Claus, Grünberger, Alexander, Drobietz, Dagmar, Hage-Hülsmann, Jennifer, rey, Georg, Büchs, Jochen, Kohlheyer, Dietrich, Loeschcke, Anita, Wiechert, Wolfgang, Jaeger, Karl-Erich, Pietruszka, Jörg, and Drepper, Thomas. “Photocaged Arabinose - A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression”. ChemBioChem 17.4 (2016): 296-299.
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8 Citations in Europe PMC

Data provided by Europe PubMed Central.

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Lee JH, Wendisch VF., Bioresour Technol 245(pt b), 2017
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Homogenizing bacterial cell factories: Analysis and engineering of phenotypic heterogeneity.
Binder D, Drepper T, Jaeger KE, Delvigne F, Wiechert W, Kohlheyer D, Grünberger A., Metab Eng 42(), 2017
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Rapid generation of recombinant Pseudomonas putida secondary metabolite producers using yTREX.
Domröse A, Weihmann R, Thies S, Jaeger KE, Drepper T, Loeschcke A., Synth Syst Biotechnol 2(4), 2017
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Light-Controlled Cell Factories: Employing Photocaged Isopropyl-β-d-Thiogalactopyranoside for Light-Mediated Optimization of lac Promoter-Based Gene Expression and (+)-Valencene Biosynthesis in Corynebacterium glutamicum.
Binder D, Frohwitter J, Mahr R, Bier C, Grünberger A, Loeschcke A, Peters-Wendisch P, Kohlheyer D, Pietruszka J, Frunzke J, Jaeger KE, Wendisch VF, Drepper T., Appl Environ Microbiol 82(20), 2016
PMID: 27520809
Comparative Single-Cell Analysis of Different E. coli Expression Systems during Microfluidic Cultivation.
Binder D, Probst C, Grünberger A, Hilgers F, Loeschcke A, Jaeger KE, Kohlheyer D, Drepper T., PLoS One 11(8), 2016
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Photocages for protection and controlled release of bioactive compounds.
Aparici-Espert I, Cuquerella MC, Paris C, Lhiaubet-Vallet V, Miranda MA., Chem Commun (Camb) 52(99), 2016
PMID: 27847958

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