Photocaged Arabinose - A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression

Binder D, Bier C, Grünberger A, Drobietz D, Hage-Hülsmann J, rey G, Büchs J, Kohlheyer D, Loeschcke A, Wiechert W, Jaeger K-E, et al. (2016)
ChemBioChem 17(4): 296-299.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
Controlling cellular functions by light allows simple triggering of biological processes in a non-invasive fashion with high spatiotemporal resolution. In this context, light-regulated gene expression has enormous potential for achieving optogenetic control over almost any cellular process. Here, we report on two novel one-step cleavable photocaged arabinose compounds, which were applied as light-sensitive inducers of transcription in bacteria. Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production, as demonstrated for GFP and the complete violacein biosynthetic pathway. Moreover, single-cell analysis revealed that intrinsic heterogeneity of arabinose-mediated induction of gene expression was overcome when using photocaged arabinose. We have thus established a novel phototrigger for synthetic bio(techno)logy applications that enables precise and homogeneous control of bacterial target gene expression.
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Zeitschriftentitel
ChemBioChem
Band
17
Zeitschriftennummer
4
Seite
296-299
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Binder D, Bier C, Grünberger A, et al. Photocaged Arabinose - A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression. ChemBioChem. 2016;17(4):296-299.
Binder, D., Bier, C., Grünberger, A., Drobietz, D., Hage-Hülsmann, J., rey, G., Büchs, J., et al. (2016). Photocaged Arabinose - A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression. ChemBioChem, 17(4), 296-299. doi:10.1002/cbic.201500609
Binder, D., Bier, C., Grünberger, A., Drobietz, D., Hage-Hülsmann, J., rey, G., Büchs, J., Kohlheyer, D., Loeschcke, A., Wiechert, W., et al. (2016). Photocaged Arabinose - A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression. ChemBioChem 17, 296-299.
Binder, D., et al., 2016. Photocaged Arabinose - A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression. ChemBioChem, 17(4), p 296-299.
D. Binder, et al., “Photocaged Arabinose - A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression”, ChemBioChem, vol. 17, 2016, pp. 296-299.
Binder, D., Bier, C., Grünberger, A., Drobietz, D., Hage-Hülsmann, J., rey, G., Büchs, J., Kohlheyer, D., Loeschcke, A., Wiechert, W., Jaeger, K.-E., Pietruszka, J., Drepper, T.: Photocaged Arabinose - A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression. ChemBioChem. 17, 296-299 (2016).
Binder, Dennis, Bier, Claus, Grünberger, Alexander, Drobietz, Dagmar, Hage-Hülsmann, Jennifer, rey, Georg, Büchs, Jochen, Kohlheyer, Dietrich, Loeschcke, Anita, Wiechert, Wolfgang, Jaeger, Karl-Erich, Pietruszka, Jörg, and Drepper, Thomas. “Photocaged Arabinose - A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression”. ChemBioChem 17.4 (2016): 296-299.

8 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Light-controlled gene expression in yeast using photocaged Cu2.
Kusen PM, Wandrey G, Krewald V, Holz M, Berstenhorst SMZ, Büchs J, Pietruszka J., J Biotechnol 258(), 2017
PMID: 28455204
Production of amino acids - Genetic and metabolic engineering approaches.
Lee JH, Wendisch VF., Bioresour Technol 245(pt b), 2017
PMID: 28552565
Taking control over microbial populations: Current approaches for exploiting biological noise in bioprocesses.
Delvigne F, Baert J, Sassi H, Fickers P, Grünberger A, Dusny C., Biotechnol J 12(7), 2017
PMID: 28544731
Homogenizing bacterial cell factories: Analysis and engineering of phenotypic heterogeneity.
Binder D, Drepper T, Jaeger KE, Delvigne F, Wiechert W, Kohlheyer D, Grünberger A., Metab Eng 42(), 2017
PMID: 28645641
Rapid generation of recombinant Pseudomonas putida secondary metabolite producers using yTREX.
Domröse A, Weihmann R, Thies S, Jaeger KE, Drepper T, Loeschcke A., Synth Syst Biotechnol 2(4), 2017
PMID: 29552656
Light-Controlled Cell Factories: Employing Photocaged Isopropyl-β-d-Thiogalactopyranoside for Light-Mediated Optimization of lac Promoter-Based Gene Expression and (+)-Valencene Biosynthesis in Corynebacterium glutamicum.
Binder D, Frohwitter J, Mahr R, Bier C, Grünberger A, Loeschcke A, Peters-Wendisch P, Kohlheyer D, Pietruszka J, Frunzke J, Jaeger KE, Wendisch VF, Drepper T., Appl Environ Microbiol 82(20), 2016
PMID: 27520809
Comparative Single-Cell Analysis of Different E. coli Expression Systems during Microfluidic Cultivation.
Binder D, Probst C, Grünberger A, Hilgers F, Loeschcke A, Jaeger KE, Kohlheyer D, Drepper T., PLoS One 11(8), 2016
PMID: 27525986
Photocages for protection and controlled release of bioactive compounds.
Aparici-Espert I, Cuquerella MC, Paris C, Lhiaubet-Vallet V, Miranda MA., Chem Commun (Camb) 52(99), 2016
PMID: 27847958

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