Real-time monitoring of fungal growth and morphogenesis at single-cell resolution

Grünberger A, Schöler K, Probst C, Kornfeld G, Hardiman T, Wiechert W, Kohlheyer D, Noack S (2017)
Engineering in life sciences 17(1): 86–92.

Download
No fulltext has been uploaded. References only!
Journal Article | Original Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ; ; ;
Abstract
Development times for efficient large-scale production, utilizing fungal species, are still very long. This is mainly due to the poor knowledge of many important variables related to fungal growth and morphogenesis. We specifically addressed this knowledge gap by combining a microfluidic cultivation device with time-lapse live cell imaging. This combination facilitates (i) studying population heterogeneity at single-cell resolution, (ii) monitoring of fungal morphogenesis in a high spatiotemporal manner under defined environmental conditions, and (iii) parallelization of experiments for statistical data analysis. Our analysis of Penicillium chrysogenum, the workhorse for antibiotic production worldwide, revealed significant heterogeneity in size, vitality and differentiation times between spore, mycelium and pellets when cultivated under industrially relevant conditions. For example, the swelling rate of single spores in complex medium (math formula) and the formation rate of higher branched mycelia in defined glucose medium (math formula) were estimated from broad time-dependent cell size distributions, which in turn were derived from computational image analysis of 257 and 49 time-lapse series, respectively. In order to speed up the development of new fungal production processes, a deeper understanding of these heterogeneities is required and the presented microfluidic single-cell approach provides a solid technical foundation for such quantitative studies.
Publishing Year
ISBN
PUB-ID

Cite this

Grünberger A, Schöler K, Probst C, et al. Real-time monitoring of fungal growth and morphogenesis at single-cell resolution. Engineering in life sciences. 2017;17(1):86–92.
Grünberger, A., Schöler, K., Probst, C., Kornfeld, G., Hardiman, T., Wiechert, W., Kohlheyer, D., et al. (2017). Real-time monitoring of fungal growth and morphogenesis at single-cell resolution. Engineering in life sciences, 17(1), 86–92. doi:10.1002/elsc.201600083
Grünberger, A., Schöler, K., Probst, C., Kornfeld, G., Hardiman, T., Wiechert, W., Kohlheyer, D., and Noack, S. (2017). Real-time monitoring of fungal growth and morphogenesis at single-cell resolution. Engineering in life sciences 17, 86–92.
Grünberger, A., et al., 2017. Real-time monitoring of fungal growth and morphogenesis at single-cell resolution. Engineering in life sciences, 17(1), p 86–92.
A. Grünberger, et al., “Real-time monitoring of fungal growth and morphogenesis at single-cell resolution”, Engineering in life sciences, vol. 17, 2017, pp. 86–92.
Grünberger, A., Schöler, K., Probst, C., Kornfeld, G., Hardiman, T., Wiechert, W., Kohlheyer, D., Noack, S.: Real-time monitoring of fungal growth and morphogenesis at single-cell resolution. Engineering in life sciences. 17, 86–92 (2017).
Grünberger, Alexander, Schöler, Katja, Probst, Christopher, Kornfeld, Georg, Hardiman, Timo, Wiechert, Wolfgang, Kohlheyer, Dietrich, and Noack, Stephan. “Real-time monitoring of fungal growth and morphogenesis at single-cell resolution”. Engineering in life sciences 17.1 (2017): 86–92.
This data publication is cited in the following publications:
This publication cites the following data publications:

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Search this title in

Google Scholar
ISBN Search