A metabolomic approach to characterize the acid-tolerance response in Sinorhizobium meliloti

Omar Draghi W, Florencia Del Papa M, Barsch A, Albicoro FJ, Lozano MJ, Pühler A, Niehaus K, Lagares A (2017)
METABOLOMICS 13(6): 71.

Download
No fulltext has been uploaded. References only!
Journal Article | Original Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ; ; ;
Abstract
Introduction Sinorhizobium meliloti establishes a symbiosis with Medicago species where the bacterium fixes atmospheric nitrogen for plant nutrition. To achieve a successful symbiosis, however, both partners need to withstand biotic and abiotic stresses within the soil, especially that of excess acid, to which the Medicago-Sinorhizobium symbiotic system is widely recognized as being highly sensitive. Objective To cope with low pH, S. meliloti can undergo an acid-tolerance response (ATR(+)) that not only enables a better survival but also constitutes a more competitive phenotype for Medicago sativa nodulation under acid and neutral conditions. To characterize this phenotype, we employed metabolomics to investigate the biochemical changes operating in ATR(+) cells. Methods A gas chromatography/mass spectrometry approach was used on S. meliloti 2011 cultures showing ATR(+) and ATR(-) phenotypes. After an univariate and multivariate statistical analysis, enzymatic activities and/or reserve carbohydrates characterizing ATR(+) phenotypes were determined. Results Two distinctive populations were clearly defined in cultures grown in acid and neutral pH based on the metabolites present. A shift occurred in the carbon-catabolic pathways, potentially supplying NAD(P)H equivalents for use in other metabolic reactions and/or for maintaining intracellular-pH homeostasis. Furthermore, among the mechanisms related to acid resistance, the ATR(+) phenotype was also characterized by lactate production, envelope modification, and carbon-overflow metabolism. Conclusions Acid-challenged S. meliloti exhibited several changes in different metabolic pathways that, in specific instances, could be identified and related to responses observed in other bacteria under various abiotic stresses. Some of the observed changes included modifications in the pentose-phosphate pathway (PPP), the exopolysaccharide biosynthesis, and in the myo-inositol degradation intermediates. Such modifications are part of a metabolic adaptation in the rhizobia that, as previously reported, is associated to improved phenotypes of acid tolerance and nodulation competitiveness.
Publishing Year
ISSN
eISSN
PUB-ID

Cite this

Omar Draghi W, Florencia Del Papa M, Barsch A, et al. A metabolomic approach to characterize the acid-tolerance response in Sinorhizobium meliloti. METABOLOMICS. 2017;13(6): 71.
Omar Draghi, W., Florencia Del Papa, M., Barsch, A., Albicoro, F. J., Lozano, M. J., Pühler, A., Niehaus, K., et al. (2017). A metabolomic approach to characterize the acid-tolerance response in Sinorhizobium meliloti. METABOLOMICS, 13(6), 71. doi:10.1007/s11306-017-1210-2
Omar Draghi, W., Florencia Del Papa, M., Barsch, A., Albicoro, F. J., Lozano, M. J., Pühler, A., Niehaus, K., and Lagares, A. (2017). A metabolomic approach to characterize the acid-tolerance response in Sinorhizobium meliloti. METABOLOMICS 13:71.
Omar Draghi, W., et al., 2017. A metabolomic approach to characterize the acid-tolerance response in Sinorhizobium meliloti. METABOLOMICS, 13(6): 71.
W. Omar Draghi, et al., “A metabolomic approach to characterize the acid-tolerance response in Sinorhizobium meliloti”, METABOLOMICS, vol. 13, 2017, : 71.
Omar Draghi, W., Florencia Del Papa, M., Barsch, A., Albicoro, F.J., Lozano, M.J., Pühler, A., Niehaus, K., Lagares, A.: A metabolomic approach to characterize the acid-tolerance response in Sinorhizobium meliloti. METABOLOMICS. 13, : 71 (2017).
Omar Draghi, Walter, Florencia Del Papa, Maria, Barsch, Aiko, Albicoro, Francisco J., Lozano, Mauricio J., Pühler, Alfred, Niehaus, Karsten, and Lagares, Antonio. “A metabolomic approach to characterize the acid-tolerance response in Sinorhizobium meliloti”. METABOLOMICS 13.6 (2017): 71.
This data publication is cited in the following publications:
This publication cites the following data publications:

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Search this title in

Google Scholar