Chip-based wide field-of-view nanoscopy

Diekmann R, Helle OI, Oie CI, McCourt P, Huser T, Schüttpelz M, Ahluwalia BS (2017)
NATURE PHOTONICS 11(5): 322-+.

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Journal Article | Original Article | Published | English

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Abstract
Present optical nanoscopy techniques use a complex microscope for imaging and a simple glass slide to hold the sample. Here, we demonstrate the inverse: the use of a complex, but mass-producible optical chip, which hosts the sample and provides a waveguide for the illumination source, and a standard low-cost microscope to acquire super-resolved images via two different approaches. Waveguides composed of a material with high refractive-index contrast provide a strong evanescent field that is used for single-molecule switching and fluorescence excitation, thus enabling chip-based single-molecule localization microscopy. Additionally, multimode interference patterns induce spatial fluorescence intensity variations that enable fluctuation-based super-resolution imaging. As chip-based nanoscopy separates the illumination and detection light paths, total-internal-reflection fluorescence excitation is possible over a large field of view, with up to 0.5 mm x 0.5 mm being demonstrated. Using multicolour chip-based nanoscopy, we visualize fenestrations in liver sinusoidal endothelial cells.
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Diekmann R, Helle OI, Oie CI, et al. Chip-based wide field-of-view nanoscopy. NATURE PHOTONICS. 2017;11(5):322-+.
Diekmann, R., Helle, O. I., Oie, C. I., McCourt, P., Huser, T., Schüttpelz, M., & Ahluwalia, B. S. (2017). Chip-based wide field-of-view nanoscopy. NATURE PHOTONICS, 11(5), 322-+. doi:10.1038/NPHOTON.2017.55
Diekmann, R., Helle, O. I., Oie, C. I., McCourt, P., Huser, T., Schüttpelz, M., and Ahluwalia, B. S. (2017). Chip-based wide field-of-view nanoscopy. NATURE PHOTONICS 11, 322-+.
Diekmann, R., et al., 2017. Chip-based wide field-of-view nanoscopy. NATURE PHOTONICS, 11(5), p 322-+.
R. Diekmann, et al., “Chip-based wide field-of-view nanoscopy”, NATURE PHOTONICS, vol. 11, 2017, pp. 322-+.
Diekmann, R., Helle, O.I., Oie, C.I., McCourt, P., Huser, T., Schüttpelz, M., Ahluwalia, B.S.: Chip-based wide field-of-view nanoscopy. NATURE PHOTONICS. 11, 322-+ (2017).
Diekmann, Robin, Helle, Oystein I., Oie, Cristina I., McCourt, Peter, Huser, Thomas, Schüttpelz, Mark, and Ahluwalia, Balpreet S. “Chip-based wide field-of-view nanoscopy”. NATURE PHOTONICS 11.5 (2017): 322-+.
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