RAD sequencing resolves fine-scale population structure in a benthic invertebrate: implications for understanding phenotypic plasticity

Vendrami D, Telesca L, Weigand H, Weiss M, Fawcett K, Lehman K, Clark MS, Leese F, McMinn C, Moore H, Hoffman J (2017)
ROYAL SOCIETY OPEN SCIENCE 4(2): 160548.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
; ; ; ; ; ; ; ; ; ;
Abstract / Bemerkung
The field of molecular ecology is transitioning from the use of small panels of classical genetic markers such as microsatellites to much larger panels of single nucleotide polymorphisms (SNPs) generated by approaches like RAD sequencing. However, few empirical studies have directly compared the ability of these methods to resolve population structure. This could have implications for understanding phenotypic plasticity, as many previous studies of natural populations may have lacked the power to detect genetic differences, especially over micro-geographic scales. We therefore compared the ability of microsatellites and RAD sequencing to resolve fine-scale population structure in a commercially important benthic invertebrate by genotyping great scallops (Pecten maximus) from nine populations around Northern Ireland at 13 microsatellites and 10 539 SNPs. The shells were then subjected to morphometric and colour analysis in order to compare patterns of phenotypic and genetic variation. We found that RAD sequencing was superior at resolving population structure, yielding higher F-st values and support for two distinct genetic clusters, whereas only one cluster could be detected in a Bayesian analysis of the microsatellite dataset. Furthermore, appreciable phenotypic variation was observed in size-independent shell shape and coloration, including among localities that could not be distinguished from one another genetically, providing support for the notion that these traits are phenotypically plastic. Taken together, our results suggest that RAD sequencing is a powerful approach for studying population structure and phenotypic plasticity in natural populations.
Erscheinungsjahr
Zeitschriftentitel
ROYAL SOCIETY OPEN SCIENCE
Band
4
Zeitschriftennummer
2
Artikelnummer
160548
ISSN
PUB-ID

Zitieren

Vendrami D, Telesca L, Weigand H, et al. RAD sequencing resolves fine-scale population structure in a benthic invertebrate: implications for understanding phenotypic plasticity. ROYAL SOCIETY OPEN SCIENCE. 2017;4(2): 160548.
Vendrami, D., Telesca, L., Weigand, H., Weiss, M., Fawcett, K., Lehman, K., Clark, M. S., et al. (2017). RAD sequencing resolves fine-scale population structure in a benthic invertebrate: implications for understanding phenotypic plasticity. ROYAL SOCIETY OPEN SCIENCE, 4(2), 160548. doi:10.1098/rsos.160548
Vendrami, D., Telesca, L., Weigand, H., Weiss, M., Fawcett, K., Lehman, K., Clark, M. S., Leese, F., McMinn, C., Moore, H., et al. (2017). RAD sequencing resolves fine-scale population structure in a benthic invertebrate: implications for understanding phenotypic plasticity. ROYAL SOCIETY OPEN SCIENCE 4:160548.
Vendrami, D., et al., 2017. RAD sequencing resolves fine-scale population structure in a benthic invertebrate: implications for understanding phenotypic plasticity. ROYAL SOCIETY OPEN SCIENCE, 4(2): 160548.
D. Vendrami, et al., “RAD sequencing resolves fine-scale population structure in a benthic invertebrate: implications for understanding phenotypic plasticity”, ROYAL SOCIETY OPEN SCIENCE, vol. 4, 2017, : 160548.
Vendrami, D., Telesca, L., Weigand, H., Weiss, M., Fawcett, K., Lehman, K., Clark, M.S., Leese, F., McMinn, C., Moore, H., Hoffman, J.: RAD sequencing resolves fine-scale population structure in a benthic invertebrate: implications for understanding phenotypic plasticity. ROYAL SOCIETY OPEN SCIENCE. 4, : 160548 (2017).
Vendrami, David, Telesca, Luca, Weigand, Hannah, Weiss, Martina, Fawcett, Katie, Lehman, Katrin, Clark, M. S., Leese, Florian, McMinn, Carrie, Moore, Heather, and Hoffman, Joseph. “RAD sequencing resolves fine-scale population structure in a benthic invertebrate: implications for understanding phenotypic plasticity”. ROYAL SOCIETY OPEN SCIENCE 4.2 (2017): 160548.

5 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

From conservation genetics to conservation genomics: a genome-wide assessment of blue whales (Balaenoptera musculus) in Australian feeding aggregations.
Attard CRM, Beheregaray LB, Sandoval-Castillo J, Jenner KCS, Gill PC, Jenner MM, Morrice MG, Möller LM., R Soc Open Sci 5(1), 2018
PMID: 29410806
Deciphering the origin of mito-nuclear discordance in two sibling caddisfly species.
Weigand H, Weiss M, Cai H, Li Y, Yu L, Zhang C, Leese F., Mol Ecol 26(20), 2017
PMID: 28792677
Molecular Ecological Basis of Grasshopper (Oedaleus asiaticus) Phenotypic Plasticity under Environmental Selection.
Qin X, Hao K, Ma J, Huang X, Tu X, Ali MP, Pittendrigh BR, Cao G, Wang G, Nong X, Whitman DW, Zhang Z., Front Physiol 8(), 2017
PMID: 29066978

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 28386419
PubMed | Europe PMC

Suchen in

Google Scholar