The AtMYB12 activation domain maps to a short C-terminal region of the transcription factor

Stracke R, Turgut-Kara N, Weisshaar B (2017)
Zeitschrift für Naturforschung C.

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Abstract
The Arabidopsis thaliana R2R3-MYB transcription factor MYB12 is a light-inducible, flavonol-specific activator of flavonoid biosynthesis. The transactivation activity of the AtMYB12 protein was analyzed using a C-terminal deletion series in a transient A. thaliana protoplast assay with the goal of mapping the activation domain (AD). Although the deletion of the last 46 C-terminal amino acids did not affect the activation capacity, the deletion of the last 98 amino acids almost totally abolished transactivation of two different target promoters. A domain swap experiment using the yeast GAL4 DNA-binding domain revealed that the region from positions 282 to 328 of AtMYB12 was sufficient for transactivation. In contrast to the R2R3-MYB ADs known thus far, that of AtMYB12 is not located at the rearmost C-terminal end of the protein. The AtMYB12 AD is conserved in other experimentally proven R2R3-MYB flavonol regulators from different species.
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Stracke R, Turgut-Kara N, Weisshaar B. The AtMYB12 activation domain maps to a short C-terminal region of the transcription factor. Zeitschrift für Naturforschung C. 2017.
Stracke, R., Turgut-Kara, N., & Weisshaar, B. (2017). The AtMYB12 activation domain maps to a short C-terminal region of the transcription factor. Zeitschrift für Naturforschung C. doi:10.1515/znc-2016-0221
Stracke, R., Turgut-Kara, N., and Weisshaar, B. (2017). The AtMYB12 activation domain maps to a short C-terminal region of the transcription factor. Zeitschrift für Naturforschung C.
Stracke, R., Turgut-Kara, N., & Weisshaar, B., 2017. The AtMYB12 activation domain maps to a short C-terminal region of the transcription factor. Zeitschrift für Naturforschung C.
R. Stracke, N. Turgut-Kara, and B. Weisshaar, “The AtMYB12 activation domain maps to a short C-terminal region of the transcription factor”, Zeitschrift für Naturforschung C, 2017.
Stracke, R., Turgut-Kara, N., Weisshaar, B.: The AtMYB12 activation domain maps to a short C-terminal region of the transcription factor. Zeitschrift für Naturforschung C. (2017).
Stracke, Ralf, Turgut-Kara, Neslihan, and Weisshaar, Bernd. “The AtMYB12 activation domain maps to a short C-terminal region of the transcription factor”. Zeitschrift für Naturforschung C (2017).
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PMID: 28284041
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