Boreal pollen contain ice-nucleating as well as ice-binding ‘antifreeze’ polysaccharides

Dreischmeier K, Budke C, Wiehemeier L, Kottke T, Koop T (2017)
Scientific Reports 7: 41890.

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Journal Article | Original Article | Published | English
Abstract
Ice nucleation and growth is an important and widespread environmental process. Accordingly, nature has developed means to either promote or inhibit ice crystal formation, for example ice-nucleating proteins in bacteria or ice-binding antifreeze proteins in polar fish. Recently, it was found that birch pollen release ice-nucleating macromolecules when suspended in water. Here we show that birch pollen washing water exhibits also ice-binding properties such as ice shaping and ice recrystallization inhibition, similar to antifreeze proteins. We present spectroscopic evidence that both the ice-nucleating as well as the ice-binding molecules are polysaccharides bearing carboxylate groups. The spectra suggest that both polysaccharides consist of very similar chemical moieties, but centrifugal filtration indicates differences in molecular size: ice nucleation occurs only in the supernatant of a 100 kDa filter, while ice shaping is strongly enhanced in the filtrate. This finding may suggest that the larger ice-nucleating polysaccharides consist of clusters of the smaller ice-binding polysaccharides, or that the latter are fragments of the ice-nucleating polysaccharides. Finally, similar polysaccharides released from pine and alder pollen also display both ice-nucleating as well as ice-binding ability, suggesting a common mechanism of interaction with ice among several boreal pollen with implications for atmospheric processes and antifreeze protection.
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Article Processing Charge funded by the Deutsche Forschungsgemeinschaft and the Open Access Publication Fund of Bielefeld University.
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Dreischmeier K, Budke C, Wiehemeier L, Kottke T, Koop T. Boreal pollen contain ice-nucleating as well as ice-binding ‘antifreeze’ polysaccharides. Scientific Reports. 2017;7: 41890.
Dreischmeier, K., Budke, C., Wiehemeier, L., Kottke, T., & Koop, T. (2017). Boreal pollen contain ice-nucleating as well as ice-binding ‘antifreeze’ polysaccharides. Scientific Reports, 7, 41890. doi:10.1038/srep41890
Dreischmeier, K., Budke, C., Wiehemeier, L., Kottke, T., and Koop, T. (2017). Boreal pollen contain ice-nucleating as well as ice-binding ‘antifreeze’ polysaccharides. Scientific Reports 7:41890.
Dreischmeier, K., et al., 2017. Boreal pollen contain ice-nucleating as well as ice-binding ‘antifreeze’ polysaccharides. Scientific Reports, 7: 41890.
K. Dreischmeier, et al., “Boreal pollen contain ice-nucleating as well as ice-binding ‘antifreeze’ polysaccharides”, Scientific Reports, vol. 7, 2017, : 41890.
Dreischmeier, K., Budke, C., Wiehemeier, L., Kottke, T., Koop, T.: Boreal pollen contain ice-nucleating as well as ice-binding ‘antifreeze’ polysaccharides. Scientific Reports. 7, : 41890 (2017).
Dreischmeier, Katharina, Budke, Carsten, Wiehemeier, Lars, Kottke, Tilman, and Koop, Thomas. “Boreal pollen contain ice-nucleating as well as ice-binding ‘antifreeze’ polysaccharides”. Scientific Reports 7 (2017): 41890.
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