A whole cell biocatalyst for double oxidation of cyclooctane

Müller CA, Weingartner AM, Dennig A, Ruff AJ, Gröger H, Schwaneberg U (2016)
Journal of Industrial Microbiology & Biotechnology 43(12): 1641-1646.

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Abstract
A novel whole cell cascade for double oxidation of cyclooctane to cyclooctanone was developed. The one-pot oxidation cascade requires only a minimum of reaction components: resting E. coli cells in aqueous buffered medium (=catalyst), the target substrate and oxygen as environmental friendly oxidant. Conversion of cyclooctane was catalysed with high efficiency (50% yield) and excellent selectivity (>94%) to cyclooctanone. The reported oxidation cascade represents a novel whole cell system for double oxidation of non-activated alkanes including an integrated cofactor regeneration. Notably, two alcohol dehydrogenases from Lactobacillus brevis and from Rhodococcus erythropolis with opposite cofactor selectivities and one monooxygenase P450 BM3 were produced in a coexpression system in one single host. The system represents the most efficient route with a TTN of up to 24363 being a promising process in terms of sustainability as well.
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Müller CA, Weingartner AM, Dennig A, Ruff AJ, Gröger H, Schwaneberg U. A whole cell biocatalyst for double oxidation of cyclooctane. Journal of Industrial Microbiology & Biotechnology. 2016;43(12):1641-1646.
Müller, C. A., Weingartner, A. M., Dennig, A., Ruff, A. J., Gröger, H., & Schwaneberg, U. (2016). A whole cell biocatalyst for double oxidation of cyclooctane. Journal of Industrial Microbiology & Biotechnology, 43(12), 1641-1646. doi:10.1007/s10295-016-1844-5
Müller, C. A., Weingartner, A. M., Dennig, A., Ruff, A. J., Gröger, H., and Schwaneberg, U. (2016). A whole cell biocatalyst for double oxidation of cyclooctane. Journal of Industrial Microbiology & Biotechnology 43, 1641-1646.
Müller, C.A., et al., 2016. A whole cell biocatalyst for double oxidation of cyclooctane. Journal of Industrial Microbiology & Biotechnology, 43(12), p 1641-1646.
C.A. Müller, et al., “A whole cell biocatalyst for double oxidation of cyclooctane”, Journal of Industrial Microbiology & Biotechnology, vol. 43, 2016, pp. 1641-1646.
Müller, C.A., Weingartner, A.M., Dennig, A., Ruff, A.J., Gröger, H., Schwaneberg, U.: A whole cell biocatalyst for double oxidation of cyclooctane. Journal of Industrial Microbiology & Biotechnology. 43, 1641-1646 (2016).
Müller, C. A., Weingartner, A. M., Dennig, A., Ruff, A. J., Gröger, Harald, and Schwaneberg, Ulrich. “A whole cell biocatalyst for double oxidation of cyclooctane”. Journal of Industrial Microbiology & Biotechnology 43.12 (2016): 1641-1646.
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PMID: 27771781
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