Nanomechanics of Fluorescent DNA Dyes on DNA Investigated by Magnetic Tweezers

Wang Y, Sischka A, Walhorn V, Tönsing K, Anselmetti D (2016)
Biophysical Journal 111(8): 1604-1611.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
Abstract / Bemerkung
Fluorescent DNA dyes are broadly used in many biotechnological applications for detecting and imaging DNA in cells and gels. Their binding alters the structural and nanomechanical properties of DNA and affects the biological processes that are associated with it. Although interaction modes like intercalation and minor groove binding already have been identified, associated mechanic effects like local elongation, unwinding, and softening of the DNA often remain in question. We used magnetic tweezers to quantitatively investigate the impact of three DNA-binding dyes (YOYO-1, DAPI, and DRAQ5) in a concentration-dependent manner. By extending and overwinding individual, torsionally constrained, nick free dsDNA molecules, we measured the contour lengths and molecular forces that allow estimation of thermodynamic and nanomechanical binding parameters. Whereas for YOYO-1 and DAPI the binding mechanisms could be assigned to bis-intercalation and minor groove binding, respectively, DRAQ5 exhibited both binding modes in a concentration-dependent manner.
Erscheinungsjahr
Zeitschriftentitel
Biophysical Journal
Band
111
Zeitschriftennummer
8
Seite
1604-1611
ISSN
eISSN
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Wang Y, Sischka A, Walhorn V, Tönsing K, Anselmetti D. Nanomechanics of Fluorescent DNA Dyes on DNA Investigated by Magnetic Tweezers. Biophysical Journal. 2016;111(8):1604-1611.
Wang, Y., Sischka, A., Walhorn, V., Tönsing, K., & Anselmetti, D. (2016). Nanomechanics of Fluorescent DNA Dyes on DNA Investigated by Magnetic Tweezers. Biophysical Journal, 111(8), 1604-1611. doi:10.1016/j.bpj.2016.08.042
Wang, Y., Sischka, A., Walhorn, V., Tönsing, K., and Anselmetti, D. (2016). Nanomechanics of Fluorescent DNA Dyes on DNA Investigated by Magnetic Tweezers. Biophysical Journal 111, 1604-1611.
Wang, Y., et al., 2016. Nanomechanics of Fluorescent DNA Dyes on DNA Investigated by Magnetic Tweezers. Biophysical Journal, 111(8), p 1604-1611.
Y. Wang, et al., “Nanomechanics of Fluorescent DNA Dyes on DNA Investigated by Magnetic Tweezers”, Biophysical Journal, vol. 111, 2016, pp. 1604-1611.
Wang, Y., Sischka, A., Walhorn, V., Tönsing, K., Anselmetti, D.: Nanomechanics of Fluorescent DNA Dyes on DNA Investigated by Magnetic Tweezers. Biophysical Journal. 111, 1604-1611 (2016).
Wang, Ying, Sischka, Andy, Walhorn, Volker, Tönsing, Katja, and Anselmetti, Dario. “Nanomechanics of Fluorescent DNA Dyes on DNA Investigated by Magnetic Tweezers”. Biophysical Journal 111.8 (2016): 1604-1611.

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Binding mechanism of PicoGreen to DNA characterized by magnetic tweezers and fluorescence spectroscopy.
Wang Y, Schellenberg H, Walhorn V, Toensing K, Anselmetti D., Eur Biophys J 46(6), 2017
PMID: 28251265

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