A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium Sinorhizobium meliloti

Draghi WO, Del Papa MF, Hellweg C, Watt SA, Watt TF, Barsch A, Lozano MJ, Lagares, A. J, Salas ME, Lopez JL, Albicoro FJ, et al. (2016)
SCIENTIFIC REPORTS 6: 29278.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
; ; ; ; ; ; ; ; ; ; ;
Alle
Abstract / Bemerkung
Abiotic stresses in general and extracellular acidity in particular disturb and limit nitrogen-fixing symbioses between rhizobia and their host legumes. Except for valuable molecular-biological studies on different rhizobia, no consolidated models have been formulated to describe the central physiologic changes that occur in acid-stressed bacteria. We present here an integrated analysis entailing the main cultural, metabolic, and molecular responses of the model bacterium Sinorhizobium meliloti growing under controlled acid stress in a chemostat. A stepwise extracellular acidification of the culture medium had indicated that S. meliloti stopped growing at ca. pH 6.0-6.1. Under such stress the rhizobia increased the O-2 consumption per cell by more than 5-fold. This phenotype, together with an increase in the transcripts for several membrane cytochromes, entails a higher aerobic-respiration rate in the acid-stressed rhizobia. Multivariate analysis of global metabolome data served to unequivocally correlate specific-metabolite profiles with the extracellular pH, showing that at low pH the pentose-phosphate pathway exhibited increases in several transcripts, enzymes, and metabolites. Further analyses should be focused on the time course of the observed changes, its associated intracellular signaling, and on the comparison with the changes that operate during the sub lethal acid-adaptive response (ATR) in rhizobia.
Erscheinungsjahr
Zeitschriftentitel
SCIENTIFIC REPORTS
Band
6
Artikelnummer
29278
ISSN
PUB-ID

Zitieren

Draghi WO, Del Papa MF, Hellweg C, et al. A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium Sinorhizobium meliloti. SCIENTIFIC REPORTS. 2016;6: 29278.
Draghi, W. O., Del Papa, M. F., Hellweg, C., Watt, S. A., Watt, T. F., Barsch, A., Lozano, M. J., et al. (2016). A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium Sinorhizobium meliloti. SCIENTIFIC REPORTS, 6, 29278
Draghi, W. O., Del Papa, M. F., Hellweg, C., Watt, S. A., Watt, T. F., Barsch, A., Lozano, M. J., Lagares, A., J., Salas, M. E., Lopez, J. L., et al. (2016). A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium Sinorhizobium meliloti. SCIENTIFIC REPORTS 6:29278.
Draghi, W.O., et al., 2016. A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium Sinorhizobium meliloti. SCIENTIFIC REPORTS, 6: 29278.
W.O. Draghi, et al., “A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium Sinorhizobium meliloti”, SCIENTIFIC REPORTS, vol. 6, 2016, : 29278.
Draghi, W.O., Del Papa, M.F., Hellweg, C., Watt, S.A., Watt, T.F., Barsch, A., Lozano, M.J., Lagares, A., J., Salas, M.E., Lopez, J.L., Albicoro, F.J., Nilsson, J.F., Torres Tejerizo, G.A., Luna, M.F., Pistorio, M., Boiardi, J.L., Pühler, A., Weidner, S., Niehaus, K., Lagares, A.: A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium Sinorhizobium meliloti. SCIENTIFIC REPORTS. 6, : 29278 (2016).
Draghi, W. O., Del Papa, M. F., Hellweg, C., Watt, S. A., Watt, T. F., Barsch, A., Lozano, M. J., Lagares, A., Jr., Salas, M. E., Lopez, J. L., Albicoro, F. J., Nilsson, J. F., Torres Tejerizo, G. A., Luna, M. F., Pistorio, M., Boiardi, J. L., Pühler, Alfred, Weidner, Stefan, Niehaus, Karsten, and Lagares, A. “A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium Sinorhizobium meliloti”. SCIENTIFIC REPORTS 6 (2016): 29278.

2 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Common dyes used to determine bacterial polysaccharides on agar are affected by medium acidification.
Hawkins JP, Geddes BA, Oresnik IJ., Can J Microbiol 63(6), 2017
PMID: 28253454

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 27404346
PubMed | Europe PMC

Suchen in

Google Scholar