Production of the marine carotenoid astaxanthin by metabolically engineered Corynebacterium glutamicum

Henke NA, Heider S, Peters-Wendisch P, Wendisch VF (2016)
Marine Drugs 14: 124.

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Journal Article | Original Article | Published | English
Abstract
Astaxanthin, a red C40 carotenoid, is one of the most abundant marine carotenoids. It is currently used as a food and feed additive in a hundred-ton scale and is furthermore an attractive component for pharmaceutical and cosmetic applications with antioxidant activities. Corynebacterium glutamicum, which naturally synthesizes the yellow C50 carotenoid decaprenoxanthin, is an industrially relevant microorganism used in the million-ton amino acid production. In this work, engineering of a genome-reduced C. glutamicum with optimized precursor supply for astaxanthin production is described. This involved expression of heterologous genes encoding for lycopene cyclase CrtY, β-carotene ketolase CrtW, and hydroxylase CrtZ. For balanced expression of crtW and crtZ their translation initiation rates were varied in a systematic approach using different ribosome binding sites, spacing, and translational start codons. Furthermore, β-carotene ketolases and hydroxylases from different marine bacteria were tested with regard to efficient astaxanthin production in C. glutamicum. In shaking flasks, the C. glutamicum strains developed here overproduced astaxanthin with volumetric productivities up to 0.4 mg·L−1·h−1 which are competitive with current algae-based production. Since C. glutamicum can grow to high cell densities of up to 100 g cell dry weight (CDW)·L−1, the recombinant strains developed here are a starting point for astaxanthin production by C. glutamicum.
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Article Processing Charge funded by the Deutsche Forschungsgemeinschaft and the Open Access Publication Fund of Bielefeld University.
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Henke NA, Heider S, Peters-Wendisch P, Wendisch VF. Production of the marine carotenoid astaxanthin by metabolically engineered Corynebacterium glutamicum. Marine Drugs. 2016;14: 124.
Henke, N. A., Heider, S., Peters-Wendisch, P., & Wendisch, V. F. (2016). Production of the marine carotenoid astaxanthin by metabolically engineered Corynebacterium glutamicum. Marine Drugs, 14, 124. doi:10.3390/md14070124
Henke, N. A., Heider, S., Peters-Wendisch, P., and Wendisch, V. F. (2016). Production of the marine carotenoid astaxanthin by metabolically engineered Corynebacterium glutamicum. Marine Drugs 14:124.
Henke, N.A., et al., 2016. Production of the marine carotenoid astaxanthin by metabolically engineered Corynebacterium glutamicum. Marine Drugs, 14: 124.
N.A. Henke, et al., “Production of the marine carotenoid astaxanthin by metabolically engineered Corynebacterium glutamicum”, Marine Drugs, vol. 14, 2016, : 124.
Henke, N.A., Heider, S., Peters-Wendisch, P., Wendisch, V.F.: Production of the marine carotenoid astaxanthin by metabolically engineered Corynebacterium glutamicum. Marine Drugs. 14, : 124 (2016).
Henke, Nadja Alina, Heider, Sabine, Peters-Wendisch, Petra, and Wendisch, Volker F. “Production of the marine carotenoid astaxanthin by metabolically engineered Corynebacterium glutamicum”. Marine Drugs 14 (2016): 124.
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