Carbohydrate-Carbohydrate Interactions Mediated by Sulfate Esters and Calcium Provide the Cell Adhesion Required for the Emergence of Early Metazoans

Vilanova E, Santos GRC, Aquino RS, Valle-Delgado JJ, Anselmetti D, Fernandez-Busquets X, Mourao PAS (2016)
JOURNAL OF BIOLOGICAL CHEMISTRY 291(18): 9425-+.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ; ;
Abstract
Early metazoans had to evolve the first cell adhesion mechanism addressed to maintain a distinctive multicellular morphology. As the oldest extant animals, sponges are good candidates for possessing remnants of the molecules responsible for this crucial evolutionary innovation. Cell adhesion in sponges is mediated by the calcium-dependent multivalent self-interactions of sulfated polysaccharides components of extracellular membrane-bound proteoglycans, namely aggregation factors. Here, we used atomic force microscopy to demonstrate that the aggregation factor of the sponge Desmapsamma anchorata has a circular supramolecular structure and that it thus belongs to the spongican family. Its sulfated polysaccharide units, which were characterized via nuclear magnetic resonance analysis, consist preponderantly of a central backbone composed of 3-alpha-Glc1 units partially sulfated at 2-and 4-positions and branches of Pyr(4,6)alpha-Gal1 -> 3-alpha-Fuc2(SO3)1 -> 3-alpha-Glc4(SO3) 1 -> 3-alpha-Glc -> 4-linked to the central alpha-Glc units. Single-molecule force measurements of self-binding forces of this sulfated polysaccharide and their chemically desulfated and carboxyl-reduced derivatives revealed that the sulfate epitopes and extracellular calcium are essential for providing the strength and stability necessary to sustain cell adhesion in sponges. We further discuss these findings within the framework of the role of molecular structures in the early evolution of metazoans.
Publishing Year
ISSN
eISSN
PUB-ID

Cite this

Vilanova E, Santos GRC, Aquino RS, et al. Carbohydrate-Carbohydrate Interactions Mediated by Sulfate Esters and Calcium Provide the Cell Adhesion Required for the Emergence of Early Metazoans. JOURNAL OF BIOLOGICAL CHEMISTRY. 2016;291(18):9425-+.
Vilanova, E., Santos, G. R. C., Aquino, R. S., Valle-Delgado, J. J., Anselmetti, D., Fernandez-Busquets, X., & Mourao, P. A. S. (2016). Carbohydrate-Carbohydrate Interactions Mediated by Sulfate Esters and Calcium Provide the Cell Adhesion Required for the Emergence of Early Metazoans. JOURNAL OF BIOLOGICAL CHEMISTRY, 291(18), 9425-+.
Vilanova, E., Santos, G. R. C., Aquino, R. S., Valle-Delgado, J. J., Anselmetti, D., Fernandez-Busquets, X., and Mourao, P. A. S. (2016). Carbohydrate-Carbohydrate Interactions Mediated by Sulfate Esters and Calcium Provide the Cell Adhesion Required for the Emergence of Early Metazoans. JOURNAL OF BIOLOGICAL CHEMISTRY 291, 9425-+.
Vilanova, E., et al., 2016. Carbohydrate-Carbohydrate Interactions Mediated by Sulfate Esters and Calcium Provide the Cell Adhesion Required for the Emergence of Early Metazoans. JOURNAL OF BIOLOGICAL CHEMISTRY, 291(18), p 9425-+.
E. Vilanova, et al., “Carbohydrate-Carbohydrate Interactions Mediated by Sulfate Esters and Calcium Provide the Cell Adhesion Required for the Emergence of Early Metazoans”, JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 291, 2016, pp. 9425-+.
Vilanova, E., Santos, G.R.C., Aquino, R.S., Valle-Delgado, J.J., Anselmetti, D., Fernandez-Busquets, X., Mourao, P.A.S.: Carbohydrate-Carbohydrate Interactions Mediated by Sulfate Esters and Calcium Provide the Cell Adhesion Required for the Emergence of Early Metazoans. JOURNAL OF BIOLOGICAL CHEMISTRY. 291, 9425-+ (2016).
Vilanova, Eduardo, Santos, Gustavo R. C., Aquino, Rafael S., Valle-Delgado, Juan J., Anselmetti, Dario, Fernandez-Busquets, Xavier, and Mourao, Paulo A. S. “Carbohydrate-Carbohydrate Interactions Mediated by Sulfate Esters and Calcium Provide the Cell Adhesion Required for the Emergence of Early Metazoans”. JOURNAL OF BIOLOGICAL CHEMISTRY 291.18 (2016): 9425-+.
This data publication is cited in the following publications:
This publication cites the following data publications:

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 26917726
PubMed | Europe PMC

Search this title in

Google Scholar