Finished genome sequence and methylome of the cyanide-degrading Pseudomonas pseudoalcaligenes strain CECT5344 as resolved by single-molecule real-time sequencing

Wibberg D, Bremges A, Dammann-Kalinowski T, Maus I, Igeño MI, Vogelsang R, König C, Luque-Almagro VM, Roldán MD, Sczyrba A, Moreno-Vivián C, et al. (2016)
Journal of Biotechnology 232: 61-68.

Download
No fulltext has been uploaded. References only!
Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ; ; ; ; ; ; ;
All
Abstract
Pseudomonas pseudoalcaligenes CECT5344 tolerates cyanide and is also able to utilize cyanide and cyano-derivatives as a nitrogen source under alkaline conditions. The strain is considered as candidate for bioremediation of habitats contaminated with cyanide-containing liquid wastes. Information on the genome sequence of the strain CECT5344 became available previously. The P. pseudoalcaligenes CECT5344 genome was now resequenced by applying the single molecule, real-time (SMRT()) sequencing technique developed by Pacific Biosciences. The complete and finished genome sequence of the strain consists of a 4,696,984 bp chromosome featuring a GC-content of 62.34%. Comparative analyses between the new and previous versions of the P. pseudoalcaligenes CECT5344 genome sequence revealed additional regions in the new sequence that were missed in the older version. These additional regions mostly represent mobile genetic elements. Moreover, five additional genes predicted to play a role in sulfoxide reduction are present in the newly established genome sequence. The P. pseudoalcaligenes CECT5344 genome sequence is highly related to the genome sequences of different Pseudomonas mendocina strains. Approximately, 70% of all genes are shared between P. pseudoalcaligenes and P. mendocina. In contrast to P. mendocina, putative pathogenicity genes were not identified in the P. pseudoalcaligenes CECT5344 genome. P. pseudoalcaligenes CECT5344 possesses unique genes for nitrilases and mercury resistance proteins that are of importance for survival in habitats contaminated with cyano- and mercury compounds. As an additional feature of the SMRT sequencing technology, the methylome of P. pseudoalcaligenes was established. Six sequence motifs featuring methylated adenine residues (m6A) were identified in the genome. The genome encodes several methyltransferases, some of which may be considered for methylation of the m6A motifs identified. The complete genome sequence of the strain CECT5344 now provides the basis for exploitation of genetic features for biotechnological purposes. Copyright 2016 Elsevier B.V. All rights reserved.
Publishing Year
ISSN
eISSN
PUB-ID

Cite this

Wibberg D, Bremges A, Dammann-Kalinowski T, et al. Finished genome sequence and methylome of the cyanide-degrading Pseudomonas pseudoalcaligenes strain CECT5344 as resolved by single-molecule real-time sequencing. Journal of Biotechnology. 2016;232:61-68.
Wibberg, D., Bremges, A., Dammann-Kalinowski, T., Maus, I., Igeño, M. I., Vogelsang, R., König, C., et al. (2016). Finished genome sequence and methylome of the cyanide-degrading Pseudomonas pseudoalcaligenes strain CECT5344 as resolved by single-molecule real-time sequencing. Journal of Biotechnology, 232, 61-68. doi:10.1016/j.jbiotec.2016.04.008
Wibberg, D., Bremges, A., Dammann-Kalinowski, T., Maus, I., Igeño, M. I., Vogelsang, R., König, C., Luque-Almagro, V. M., Roldán, M. D., Sczyrba, A., et al. (2016). Finished genome sequence and methylome of the cyanide-degrading Pseudomonas pseudoalcaligenes strain CECT5344 as resolved by single-molecule real-time sequencing. Journal of Biotechnology 232, 61-68.
Wibberg, D., et al., 2016. Finished genome sequence and methylome of the cyanide-degrading Pseudomonas pseudoalcaligenes strain CECT5344 as resolved by single-molecule real-time sequencing. Journal of Biotechnology, 232, p 61-68.
D. Wibberg, et al., “Finished genome sequence and methylome of the cyanide-degrading Pseudomonas pseudoalcaligenes strain CECT5344 as resolved by single-molecule real-time sequencing”, Journal of Biotechnology, vol. 232, 2016, pp. 61-68.
Wibberg, D., Bremges, A., Dammann-Kalinowski, T., Maus, I., Igeño, M.I., Vogelsang, R., König, C., Luque-Almagro, V.M., Roldán, M.D., Sczyrba, A., Moreno-Vivián, C., Blasco, R., Pühler, A., Schlüter, A.: Finished genome sequence and methylome of the cyanide-degrading Pseudomonas pseudoalcaligenes strain CECT5344 as resolved by single-molecule real-time sequencing. Journal of Biotechnology. 232, 61-68 (2016).
Wibberg, Daniel, Bremges, Andreas, Dammann-Kalinowski, Tanja, Maus, Irena, Igeño, Maria Isabel, Vogelsang, Ralph, König, Christoph, Luque-Almagro, Víctor M., Roldán, Maria Dolores, Sczyrba, Alexander, Moreno-Vivián, Conrado, Blasco, Rafael, Pühler, Alfred, and Schlüter, Andreas. “Finished genome sequence and methylome of the cyanide-degrading Pseudomonas pseudoalcaligenes strain CECT5344 as resolved by single-molecule real-time sequencing”. Journal of Biotechnology 232 (2016): 61-68.
This data publication is cited in the following publications:
This publication cites the following data publications:

3 Citations in Europe PMC

Data provided by Europe PubMed Central.

Assimilation of cyanide and cyano-derivatives by Pseudomonas pseudoalcaligenes CECT5344: from omic approaches to biotechnological applications.
Cabello P, Luque-Almagro VM, Olaya-Abril A, Sáez LP, Moreno-Vivián C, Roldán MD., FEMS Microbiol Lett 365(6), 2018
PMID: 29438505
Quantitative proteomic analysis of Pseudomonas pseudoalcaligenes CECT5344 in response to industrial cyanide-containing wastewaters using Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS).
Ibáñez MI, Cabello P, Luque-Almagro VM, Sáez LP, Olaya A, Sánchez de Medina V, Luque de Castro MD, Moreno-Vivián C, Roldán MD., PLoS One 12(3), 2017
PMID: 28253357
Multi-omics reveal the lifestyle of the acidophilic, mineral-oxidizing model species Leptospirillum ferriphilumT.
Christel S, Herold M, Bellenberg S, El Hajjami M, Buetti-Dinh A, Pivkin IV, Sand W, Wilmes P, Poetsch A, Dopson M., Appl Environ Microbiol (), 2017
PMID: 29150517

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 27060556
PubMed | Europe PMC

Search this title in

Google Scholar