Monitoring Alternative Splicing Changes in Arabidopsis Circadian Clock Genes

Simpson CG, Fuller J, Calixto CPG, McNicol J, Booth C, Brown JWS, Staiger D (2016)
In: Environmental Responses in Plants. Methods and Protocols. Duque P (Ed); Methods in Molecular Biology, 1398. New York, NY: Springer Science + Business Media: 119-132.

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Duque, Paula
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Posttranscriptional control makes an important contribution to circadian regulation of gene expression. In higher plants, alternative splicing is particularly prevalent upon abiotic and biotic stress and in the circadian system. Here we describe in detail a high-resolution reverse transcription-PCR based panel (HR RT-PCR) to monitor alternative splicing events. The use of the panel allows the quantification of changes in the proportion of splice isoforms between different samples, e.g., different time points, different tissues, genotypes, ecotypes, or treatments.
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Simpson CG, Fuller J, Calixto CPG, et al. Monitoring Alternative Splicing Changes in Arabidopsis Circadian Clock Genes. In: Duque P, ed. Environmental Responses in Plants. Methods and Protocols. Methods in Molecular Biology. Vol 1398. New York, NY: Springer Science + Business Media; 2016: 119-132.
Simpson, C. G., Fuller, J., Calixto, C. P. G., McNicol, J., Booth, C., Brown, J. W. S., & Staiger, D. (2016). Monitoring Alternative Splicing Changes in Arabidopsis Circadian Clock Genes. In P. Duque (Ed.), Methods in Molecular Biology: Vol. 1398. Environmental Responses in Plants. Methods and Protocols (pp. 119-132). New York, NY: Springer Science + Business Media.
Simpson, C. G., Fuller, J., Calixto, C. P. G., McNicol, J., Booth, C., Brown, J. W. S., and Staiger, D. (2016). “Monitoring Alternative Splicing Changes in Arabidopsis Circadian Clock Genes” in Environmental Responses in Plants. Methods and Protocols, ed. P. Duque Methods in Molecular Biology, vol. 1398, (New York, NY: Springer Science + Business Media), 119-132.
Simpson, C.G., et al., 2016. Monitoring Alternative Splicing Changes in Arabidopsis Circadian Clock Genes. In P. Duque, ed. Environmental Responses in Plants. Methods and Protocols. Methods in Molecular Biology. no.1398 New York, NY: Springer Science + Business Media, pp. 119-132.
C.G. Simpson, et al., “Monitoring Alternative Splicing Changes in Arabidopsis Circadian Clock Genes”, Environmental Responses in Plants. Methods and Protocols, P. Duque, ed., Methods in Molecular Biology, vol. 1398, New York, NY: Springer Science + Business Media, 2016, pp.119-132.
Simpson, C.G., Fuller, J., Calixto, C.P.G., McNicol, J., Booth, C., Brown, J.W.S., Staiger, D.: Monitoring Alternative Splicing Changes in Arabidopsis Circadian Clock Genes. In: Duque, P. (ed.) Environmental Responses in Plants. Methods and Protocols. Methods in Molecular Biology. 1398, p. 119-132. Springer Science + Business Media, New York, NY (2016).
Simpson, Craig G., Fuller, John, Calixto, Cristiane P. G., McNicol, Jim, Booth, Clare, Brown, John W. S., and Staiger, Dorothee. “Monitoring Alternative Splicing Changes in Arabidopsis Circadian Clock Genes”. Environmental Responses in Plants. Methods and Protocols. Ed. Paula Duque. New York, NY: Springer Science + Business Media, 2016.Vol. 1398. Methods in Molecular Biology. 119-132.
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