Multimodal super-resolution optical microscopy visualizes the close connection between membrane and the cytoskeleton in liver sinusoidal endothelial cell fenestrations

Mönkemöller V, Øie C, Hübner W, Huser T, McCourt P (2015)
Scientific Reports 5: 16279.

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Liver sinusoidal endothelial cells (LSECs) act as a filter between blood and the hepatocytes. LSECs are highly fenestrated cells; they contain transcellular pores with diameters between 50 to 200 nm. The small sizes of the fenestrae have so far prohibited any functional analysis with standard and advanced light microscopy techniques. Only the advent of super-resolution optical fluorescence microscopy now permits the recording of such small cellular structures. Here, we demonstrate the complementary use of two different super-resolution optical microscopy modalities, 3D structured illumination microscopy (3D-SIM) and single molecule localization microscopy in a common optical platform to obtain new insights into the association between the cytoskeleton and the plasma membrane that supports the formation of fenestrations. We applied 3D-SIM to multi-color stained LSECs to acquire highly resolved overviews of large sample areas. We then further increased the spatial resolution for imaging fenestrations by single molecule localization microscopy applied to select small locations of interest in the same sample on the same microscope setup. We optimized the use of fluorescent membrane stains for these imaging conditions. The combination of these techniques offers a unique opportunity to significantly improve studies of subcellular ultrastructures such as LSEC fenestrations.
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Scientific Reports
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5
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16279
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Article Processing Charge funded by the Deutsche Forschungsgemeinschaft and the Open Access Publication Fund of Bielefeld University.
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Mönkemöller V, Øie C, Hübner W, Huser T, McCourt P. Multimodal super-resolution optical microscopy visualizes the close connection between membrane and the cytoskeleton in liver sinusoidal endothelial cell fenestrations. Scientific Reports. 2015;5: 16279.
Mönkemöller, V., Øie, C., Hübner, W., Huser, T., & McCourt, P. (2015). Multimodal super-resolution optical microscopy visualizes the close connection between membrane and the cytoskeleton in liver sinusoidal endothelial cell fenestrations. Scientific Reports, 5, 16279. doi:10.1038/srep16279
Mönkemöller, V., Øie, C., Hübner, W., Huser, T., and McCourt, P. (2015). Multimodal super-resolution optical microscopy visualizes the close connection between membrane and the cytoskeleton in liver sinusoidal endothelial cell fenestrations. Scientific Reports 5:16279.
Mönkemöller, V., et al., 2015. Multimodal super-resolution optical microscopy visualizes the close connection between membrane and the cytoskeleton in liver sinusoidal endothelial cell fenestrations. Scientific Reports, 5: 16279.
V. Mönkemöller, et al., “Multimodal super-resolution optical microscopy visualizes the close connection between membrane and the cytoskeleton in liver sinusoidal endothelial cell fenestrations”, Scientific Reports, vol. 5, 2015, : 16279.
Mönkemöller, V., Øie, C., Hübner, W., Huser, T., McCourt, P.: Multimodal super-resolution optical microscopy visualizes the close connection between membrane and the cytoskeleton in liver sinusoidal endothelial cell fenestrations. Scientific Reports. 5, : 16279 (2015).
Mönkemöller, Viola, Øie, Cristina, Hübner, Wolfgang, Huser, Thomas, and McCourt, Peter. “Multimodal super-resolution optical microscopy visualizes the close connection between membrane and the cytoskeleton in liver sinusoidal endothelial cell fenestrations”. Scientific Reports 5 (2015): 16279.
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2016-02-24T12:56:03Z

5 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Liver sinusoidal endothelial cells: Physiology and role in liver diseases.
Poisson J, Lemoinne S, Boulanger C, Durand F, Moreau R, Valla D, Rautou PE., J Hepatol 66(1), 2017
PMID: 27423426
Label-free spectroscopic characterization of live liver sinusoidal endothelial cells (LSECs) isolated from the murine liver.
Kochan K, Kus E, Filipek A, Szafrańska K, Chlopicki S, Baranska M., Analyst 142(8), 2017
PMID: 27781217
Morphology and force probing of primary murine liver sinusoidal endothelial cells.
Zapotoczny B, Owczarczyk K, Szafranska K, Kus E, Chlopicki S, Szymonski M., J Mol Recognit 30(7), 2017
PMID: 28120483
Plumbagin Alleviates Capillarization of Hepatic Sinusoids In Vitro by Downregulating ET-1, VEGF, LN, and Type IV Collagen.
Li G, Peng Y, Zhao T, Lin J, Duan X, Wei Y, Ma J., Biomed Res Int 2017(), 2017
PMID: 28770223
Open-source image reconstruction of super-resolution structured illumination microscopy data in ImageJ.
Müller M, Mönkemöller V, Hennig S, Hübner W, Huser T., Nat Commun 7(), 2016
PMID: 26996201

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