Intercellular wiring enables electron transfer between methanotrophic archaea and bacteria.

Wegener G, Krukenberg V, Riedel D, Tegetmeyer H, Boetius A (2015)
Nature 526(7574): 587-590.

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Abstract
The anaerobic oxidation of methane (AOM) with sulfate controls the emission of the greenhouse gas methane from the ocean floor. In marine sediments, AOM is performed by dual-species consortia of anaerobic methanotrophic archaea (ANME) and sulfate-reducing bacteria (SRB) inhabiting the methane-sulfate transition zone. The biochemical pathways and biological adaptations enabling this globally relevant process are not fully understood. Here we study the syntrophic interaction in thermophilic AOM (TAOM) between ANME-1 archaea and their consortium partner SRB HotSeep-1 (ref. 6) at 60 °C to test the hypothesis of a direct interspecies exchange of electrons. The activity of TAOM consortia was compared to the first ANME-free culture of an AOM partner bacterium that grows using hydrogen as the sole electron donor. The thermophilic ANME-1 do not produce sufficient hydrogen to sustain the observed growth of the HotSeep-1 partner. Enhancing the growth of the HotSeep-1 partner by hydrogen addition represses methane oxidation and the metabolic activity of ANME-1. Further supporting the hypothesis of direct electron transfer between the partners, we observe that under TAOM conditions, both ANME and the HotSeep-1 bacteria overexpress genes for extracellular cytochrome production and form cell-to-cell connections that resemble the nanowire structures responsible for interspecies electron transfer between syntrophic consortia of Geobacter. HotSeep-1 highly expresses genes for pili production only during consortial growth using methane, and the nanowire-like structures are absent in HotSeep-1 cells isolated with hydrogen. These observations suggest that direct electron transfer is a principal mechanism in TAOM, which may also explain the enigmatic functioning and specificity of other methanotrophic ANME-SRB consortia.
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Wegener G, Krukenberg V, Riedel D, Tegetmeyer H, Boetius A. Intercellular wiring enables electron transfer between methanotrophic archaea and bacteria. Nature. 2015;526(7574):587-590.
Wegener, G., Krukenberg, V., Riedel, D., Tegetmeyer, H., & Boetius, A. (2015). Intercellular wiring enables electron transfer between methanotrophic archaea and bacteria. Nature, 526(7574), 587-590. doi:10.1038/nature15733
Wegener, G., Krukenberg, V., Riedel, D., Tegetmeyer, H., and Boetius, A. (2015). Intercellular wiring enables electron transfer between methanotrophic archaea and bacteria. Nature 526, 587-590.
Wegener, G., et al., 2015. Intercellular wiring enables electron transfer between methanotrophic archaea and bacteria. Nature, 526(7574), p 587-590.
G. Wegener, et al., “Intercellular wiring enables electron transfer between methanotrophic archaea and bacteria.”, Nature, vol. 526, 2015, pp. 587-590.
Wegener, G., Krukenberg, V., Riedel, D., Tegetmeyer, H., Boetius, A.: Intercellular wiring enables electron transfer between methanotrophic archaea and bacteria. Nature. 526, 587-590 (2015).
Wegener, Gunter, Krukenberg, Viola, Riedel, Dietmar, Tegetmeyer, Halina, and Boetius, Antje. “Intercellular wiring enables electron transfer between methanotrophic archaea and bacteria.”. Nature 526.7574 (2015): 587-590.
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3 Citations in Europe PMC

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Metabolic Capabilities of Microorganisms Involved in and Associated with the Anaerobic Oxidation of Methane.
Wegener G, Krukenberg V, Ruff SE, Kellermann MY, Knittel K., Front Microbiol 7(), 2016
PMID: 26870011
Microbial Communities in Methane- and Short Chain Alkane-Rich Hydrothermal Sediments of Guaymas Basin.
Dowell F, Cardman Z, Dasarathy S, Kellermann MY, Lipp JS, Ruff SE, Biddle JF, McKay LJ, MacGregor BJ, Lloyd KG, Albert DB, Mendlovitz H, Hinrichs KU, Teske A., Front Microbiol 7(), 2016
PMID: 26858698
Microbiology: Conductive consortia.
Wagner M., Nature 526(7574), 2015
PMID: 26490616

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