Identification of two mutations increasing the methanol tolerance of *Corynebacterium glutamicum*

Leßmeier L, Wendisch VF (2015)
BMC Microbiology 15: 216.

Download
OA
Journal Article | Published | English
Abstract
Background Methanol is present in most ecosystems and may also occur in industrial applications, e.g. as an impurity of carbon sources such as technical glycerol. Methanol often inhibits growth of bacteria, thus, methanol tolerance may limit fermentative production processes. Results The methanol tolerance of the amino acid producing soil bacterium Corynebacterium glutamicum was improved by experimental evolution in the presence of methanol. The resulting strain Tol1 exhibited significantly increased growth rates in the presence of up to 1 M methanol. However, neither transcriptional changes nor increased enzyme activities of the linear methanol oxidation pathway were observed, which was in accordance with the finding that tolerance to the downstream metabolites formaldehyde and formate was not improved. Genome sequence analysis of strain Tol1 revealed two point mutations potentially relevant to enhanced methanol tolerance: one leading to the amino acid exchange A165T of O-acetylhomoserine sulfhydrolase MetY and the other leading to shortened CoA transferase Cat (Q342*). Introduction of either mutation into the genome of C. glutamicum wild type increased methanol tolerance and introduction of both mutations into C. glutamicum was sufficient to achieve methanol tolerance almost indistinguishable from that of strain Tol1. Conclusion The methanol tolerance of C. glutamicum can be increased by two point mutations leading to amino acid exchange of O-acetylhomoserine sulfhydrolase MetY and shortened CoA transferase Cat. Introduction of these mutations into producer strains may be helpful when using carbon sources containing methanol as component or impurity.
Publishing Year
ISSN
Financial disclosure
Article Processing Charge funded by the Deutsche Forschungsgemeinschaft and the Open Access Publication Fund of Bielefeld University.
PUB-ID

Cite this

Leßmeier L, Wendisch VF. Identification of two mutations increasing the methanol tolerance of *Corynebacterium glutamicum*. BMC Microbiology. 2015;15: 216.
Leßmeier, L., & Wendisch, V. F. (2015). Identification of two mutations increasing the methanol tolerance of *Corynebacterium glutamicum*. BMC Microbiology, 15: 216.
Leßmeier, L., and Wendisch, V. F. (2015). Identification of two mutations increasing the methanol tolerance of *Corynebacterium glutamicum*. BMC Microbiology 15:216.
Leßmeier, L., & Wendisch, V.F., 2015. Identification of two mutations increasing the methanol tolerance of *Corynebacterium glutamicum*. BMC Microbiology, 15: 216.
L. Leßmeier and V.F. Wendisch, “Identification of two mutations increasing the methanol tolerance of *Corynebacterium glutamicum*”, BMC Microbiology, vol. 15, 2015, : 216.
Leßmeier, L., Wendisch, V.F.: Identification of two mutations increasing the methanol tolerance of *Corynebacterium glutamicum*. BMC Microbiology. 15, : 216 (2015).
Leßmeier, Lennart, and Wendisch, Volker F. “Identification of two mutations increasing the methanol tolerance of *Corynebacterium glutamicum*”. BMC Microbiology 15 (2015): 216.
Main File(s)
Access Level
OA Open Access
Last Uploaded
2016-11-10T07:29:17Z

This data publication is cited in the following publications:
This publication cites the following data publications:

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 26474849
PubMed | Europe PMC

Search this title in

Google Scholar