Physicochemical and biological characterization of chitosan-microRNA nanocomplexes for gene delivery to MCF-7 breast cancer cells

Santos-Carballal B, Aaldering LJ, Ritzefeld M, Pereira S, Sewald N, Moerschbacher BM, Goette M, Goycoolea FM (2015)
Scientific Reports 5: 13567.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
Cancer gene therapy requires the design of non-viral vectors that carry genetic material and selectively deliver it with minimal toxicity. Non-viral vectors based on cationic natural polymers can form electrostatic complexes with negatively-charged polynucleotides such as microRNAs (miRNAs). Here we investigated the physicochemical/biophysical properties of chitosan-hsa-miRNA-145 (CS-miRNA) nanocomplexes and the biological responses of MCF-7 breast cancer cells cultured in vitro. Self-assembled CS-miRNA nanocomplexes were produced with a range of (+/-) charge ratios (from 0.6 to 8) using chitosans with various degrees of acetylation and molecular weight. The Z-average particle diameter of the complexes was <200 nm. The surface charge increased with increasing amount of chitosan. We observed that chitosan induces the base-stacking of miRNA in a concentration dependent manner. Surface plasmon resonance spectroscopy shows that complexes formed by low degree of acetylation chitosans are highly stable, regardless of the molecular weight. We found no evidence that these complexes were cytotoxic towards MCF-7 cells. Furthermore, CS-miRNA nanocomplexes with degree of acetylation 12% and 29% were biologically active, showing successful downregulation of target mRNA expression in MCF-7 cells. Our data, therefore, shows that CS-miRNA complexes offer a promising non-viral platform for breast cancer gene therapy.
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Scientific Reports
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5
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13567
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Santos-Carballal B, Aaldering LJ, Ritzefeld M, et al. Physicochemical and biological characterization of chitosan-microRNA nanocomplexes for gene delivery to MCF-7 breast cancer cells. Scientific Reports. 2015;5:13567.
Santos-Carballal, B., Aaldering, L. J., Ritzefeld, M., Pereira, S., Sewald, N., Moerschbacher, B. M., Goette, M., et al. (2015). Physicochemical and biological characterization of chitosan-microRNA nanocomplexes for gene delivery to MCF-7 breast cancer cells. Scientific Reports, 5, 13567. doi:10.1038/srep13567
Santos-Carballal, B., Aaldering, L. J., Ritzefeld, M., Pereira, S., Sewald, N., Moerschbacher, B. M., Goette, M., and Goycoolea, F. M. (2015). Physicochemical and biological characterization of chitosan-microRNA nanocomplexes for gene delivery to MCF-7 breast cancer cells. Scientific Reports 5, 13567.
Santos-Carballal, B., et al., 2015. Physicochemical and biological characterization of chitosan-microRNA nanocomplexes for gene delivery to MCF-7 breast cancer cells. Scientific Reports, 5, p 13567.
B. Santos-Carballal, et al., “Physicochemical and biological characterization of chitosan-microRNA nanocomplexes for gene delivery to MCF-7 breast cancer cells”, Scientific Reports, vol. 5, 2015, pp. 13567.
Santos-Carballal, B., Aaldering, L.J., Ritzefeld, M., Pereira, S., Sewald, N., Moerschbacher, B.M., Goette, M., Goycoolea, F.M.: Physicochemical and biological characterization of chitosan-microRNA nanocomplexes for gene delivery to MCF-7 breast cancer cells. Scientific Reports. 5, 13567 (2015).
Santos-Carballal, B., Aaldering, L. J., Ritzefeld, Markus, Pereira, S., Sewald, Norbert, Moerschbacher, B. M., Goette, M., and Goycoolea, F. M. “Physicochemical and biological characterization of chitosan-microRNA nanocomplexes for gene delivery to MCF-7 breast cancer cells”. Scientific Reports 5 (2015): 13567.

15 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

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Biopolymer-Based Nanoparticles for Cystic Fibrosis Lung Gene Therapy Studies.
Fernández Fernández E, Santos-Carballal B, de Santi C, Ramsey JM, MacLoughlin R, Cryan SA, Greene CM., Materials (Basel) 11(1), 2018
PMID: 29342838
An investigation of the interactions between an E. coli bacterial quorum sensing biosensor and chitosan-based nanocapsules.
Qin X, Engwer C, Desai S, Vila-Sanjurjo C, Goycoolea FM., Colloids Surf B Biointerfaces 149(), 2017
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In Vitro Dose Studies on Chitosan Nanoplexes for microRNA Delivery in Breast Cancer Cells.
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Biodegradable nano-polymers as delivery vehicles for therapeutic small non-coding ribonucleic acids.
Mokhtarzadeh A, Alibakhshi A, Hashemi M, Hejazi M, Hosseini V, de la Guardia M, Ramezani M., J Control Release 245(), 2017
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Nanocarriers for microRNA delivery in cancer medicine.
Fernandez-Piñeiro I, Badiola I, Sanchez A., Biotechnol Adv 35(3), 2017
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Therapeutic targeting of non-coding RNAs in cancer.
Slaby O, Laga R, Sedlacek O., Biochem J 474(24), 2017
PMID: 29242381
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Santos-Carballal B, Swamy MJ, Moerschbacher BM, Goycoolea FM., J Fluoresc 26(1), 2016
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Fernández Fernández E, Santos-Carballal B, Weber WM, Goycoolea FM., Int J Pharm 502(1-2), 2016
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Omics for Investigating Chitosan as an Antifungal and Gene Modulator.
Lopez-Moya F, Lopez-Llorca LV., J Fungi (Basel) 2(1), 2016
PMID: 29376928
Chitosan/Sterculia striata polysaccharides nanocomplex as a potential chloroquine drug release device.
Magalhães GA, Moura Neto E, Sombra VG, Richter AR, Abreu CM, Feitosa JP, Paula HC, Goycoolea FM, de Paula RC., Int J Biol Macromol 88(), 2016
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Schwickert A, Weghake E, Brüggemann K, Engbers A, Brinkmann BF, Kemper B, Seggewiß J, Stock C, Ebnet K, Kiesel L, Riethmüller C, Götte M., PLoS One 10(12), 2015
PMID: 26657485

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