Escherichia coli as host for membrane protein structure determination: a global analysis

Hattab G, Warschawski DE, Moncoq K, Miroux B (2015)
Scientific Reports 5.

Journal Article | Published | English

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Abstract
The structural biology of membrane proteins (MP) is hampered by the difficulty in producing and purifying them. A comprehensive analysis of protein databases revealed that 213 unique membrane protein structures have been obtained after production of the target protein in E. coli. The primary expression system used was the one based on the T7 RNA polymerase, followed by the arabinose and T5 promoter based expression systems. The C41λ(DE3) and C43λ(DE3) bacterial mutant hosts have contributed to 28% of non E. coli membrane protein structures. A large scale analysis of expression protocols demonstrated a preference for a combination of bacterial host-vector together with a bimodal distribution of induction temperature and of inducer concentration. Altogether our analysis provides a set of rules for the optimal use of bacterial expression systems in membrane protein production.
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Hattab G, Warschawski DE, Moncoq K, Miroux B. Escherichia coli as host for membrane protein structure determination: a global analysis. Scientific Reports. 2015;5.
Hattab, G., Warschawski, D. E., Moncoq, K., & Miroux, B. (2015). Escherichia coli as host for membrane protein structure determination: a global analysis. Scientific Reports, 5.
Hattab, G., Warschawski, D. E., Moncoq, K., and Miroux, B. (2015). Escherichia coli as host for membrane protein structure determination: a global analysis. Scientific Reports 5.
Hattab, G., et al., 2015. Escherichia coli as host for membrane protein structure determination: a global analysis. Scientific Reports, 5.
G. Hattab, et al., “Escherichia coli as host for membrane protein structure determination: a global analysis”, Scientific Reports, vol. 5, 2015.
Hattab, G., Warschawski, D.E., Moncoq, K., Miroux, B.: Escherichia coli as host for membrane protein structure determination: a global analysis. Scientific Reports. 5, (2015).
Hattab, Georges, Warschawski, Dror E., Moncoq, Karine, and Miroux, Bruno. “Escherichia coli as host for membrane protein structure determination: a global analysis”. Scientific Reports 5 (2015).
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Pechmann S, Chartron JW, Frydman J., Nat. Struct. Mol. Biol. 21(12), 2014
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