Nanoparticles as Nonfluorescent Analogues of Fluorophores for Optical Nanoscopy

Hennig S, Mönkemöller V, Böger C, Müller M, Huser T (2015)
ACS Nano 9(6): 6196-6205.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
Abstract / Bemerkung
Optical microscopy modalities that achieve spatial resolution beyond the resolution limit have opened up new opportunities in the biomedical sciences to reveal the structure and kinetics of biological processes on the nanoscale. These methods are, however, mostly restricted to fluorescence as Contrast mechanism, which limits the ultimate spatial resolution and observation time that can be achieved by photobleaching of the fluorescent probes. Here, we demonstrate that Raman scattering provides a valuable contrast mechanism for optical nanoscopy in the form of super-resolution to structured illumination microscopy. We find that nanotags, i.e., gold and silver nanoparticles that are capable of surface-enhanced Raman scattering (SERS), can be imaged with a spatial resolution beyond the diffraction limit in four dimensions alongside and with similar excitation power as fluorescent probes. The highly polarized nature of super-resolution structured illumination microscopy renders these nanotags elliptical in the reconstructed super-resolved images, which enables us to determine their orientation within the sample. The robustness of nanotags against photobleaching allows us to image these particles for unlimited periods of time. We demonstrate this by imaging isolated nanotags in a dense layer of fluorophores, as well as on the surface of and after internalization by osteosarcoma cells, always in the presence of fluorescent probes. Our results show that SERS nanotags have the potential to become highly multiplexed and chemically sensitive optical probes for optical nanoscopy that can replace fluorophores in applications where fluorescence photobleaching is prohibitive for following the evolution of biological processes for extended times.
Erscheinungsjahr
Zeitschriftentitel
ACS Nano
Band
9
Zeitschriftennummer
6
Seite
6196-6205
ISSN
PUB-ID

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Hennig S, Mönkemöller V, Böger C, Müller M, Huser T. Nanoparticles as Nonfluorescent Analogues of Fluorophores for Optical Nanoscopy. ACS Nano. 2015;9(6):6196-6205.
Hennig, S., Mönkemöller, V., Böger, C., Müller, M., & Huser, T. (2015). Nanoparticles as Nonfluorescent Analogues of Fluorophores for Optical Nanoscopy. ACS Nano, 9(6), 6196-6205. doi:10.1021/acsnano.5b01503
Hennig, S., Mönkemöller, V., Böger, C., Müller, M., and Huser, T. (2015). Nanoparticles as Nonfluorescent Analogues of Fluorophores for Optical Nanoscopy. ACS Nano 9, 6196-6205.
Hennig, S., et al., 2015. Nanoparticles as Nonfluorescent Analogues of Fluorophores for Optical Nanoscopy. ACS Nano, 9(6), p 6196-6205.
S. Hennig, et al., “Nanoparticles as Nonfluorescent Analogues of Fluorophores for Optical Nanoscopy”, ACS Nano, vol. 9, 2015, pp. 6196-6205.
Hennig, S., Mönkemöller, V., Böger, C., Müller, M., Huser, T.: Nanoparticles as Nonfluorescent Analogues of Fluorophores for Optical Nanoscopy. ACS Nano. 9, 6196-6205 (2015).
Hennig, Simon, Mönkemöller, Viola, Böger, Carolin, Müller, Marcel, and Huser, Thomas. “Nanoparticles as Nonfluorescent Analogues of Fluorophores for Optical Nanoscopy”. ACS Nano 9.6 (2015): 6196-6205.

5 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

SERS Sensors: Recent Developments and a Generalized Classification Scheme Based on the Signal Origin.
Gu X, Trujillo MJ, Olson JE, Camden JP., Annu Rev Anal Chem (Palo Alto Calif) 11(1), 2018
PMID: 29547340
Nanoparticles for super-resolution microscopy and single-molecule tracking.
Jin D, Xi P, Wang B, Zhang L, Enderlein J, van Oijen AM., Nat Methods 15(6), 2018
PMID: 29808018
Plasmon-enhanced Raman spectroscopic metrics for in situ quantitative and dynamic assays of cell apoptosis and necrosis.
Kang B, Li SS, Guan QY, Chen AP, Zhang PK, Zhang LB, Wei JW, Xu JJ, Chen HY., Chem Sci 8(2), 2017
PMID: 28451266
Open-source image reconstruction of super-resolution structured illumination microscopy data in ImageJ.
Müller M, Mönkemöller V, Hennig S, Hübner W, Huser T., Nat Commun 7(), 2016
PMID: 26996201

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