Fractionation of biogas plant sludge material improves metaproteomic characterization to investigate metabolic activity of microbial communities

Kohrs F, Wolter S, Benndorf D, Heyer R, Hoffmann M, Rapp E, Bremges A, Sczyrba A, Schlüter A, Reichl U (2015)
Proteomics 15(20): 3585-3589.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
With the development of high resolving mass spectrometers, metaproteomics evolved as a powerful tool to elucidate metabolic activity of microbial communities derived from full-scale biogas plants. Due to the vast complexity of these microbiomes, application of suitable fractionation methods are indispensable, but often turn out to be time and cost intense, depending on the method used for protein separation. In this study, centrifugal fractionation has been applied for fractionation of two biogas sludge samples to analyze proteins extracted from (i) crude fibers, (ii) suspended microorganisms, and (iii) secreted proteins in the supernatant using a gel-based approach followed by LC-MS/MS identification. This fast and easy method turned out to be beneficial to both the quality of SDS-PAGE and the identification of peptides and proteins compared to untreated samples. Additionally, a high functional metabolic pathway coverage was achieved by combining protein hits found exclusively in distinct fractions. Sample preparation using centrifugal fractionation influenced significantly the number and the types of proteins identified in the microbial metaproteomes. Thereby, comparing results from different proteomic or genomic studies, the impact of sample preparation should be considered. All MS data have been deposited in the ProteomeXchange with identifier PXD001508 (http://proteomecentral.proteomexchange.org/dataset/PXD001508). 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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Zeitschriftentitel
Proteomics
Band
15
Zeitschriftennummer
20
Seite
3585-3589
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Kohrs F, Wolter S, Benndorf D, et al. Fractionation of biogas plant sludge material improves metaproteomic characterization to investigate metabolic activity of microbial communities. Proteomics. 2015;15(20):3585-3589.
Kohrs, F., Wolter, S., Benndorf, D., Heyer, R., Hoffmann, M., Rapp, E., Bremges, A., et al. (2015). Fractionation of biogas plant sludge material improves metaproteomic characterization to investigate metabolic activity of microbial communities. Proteomics, 15(20), 3585-3589. doi:10.1002/pmic.201400557
Kohrs, F., Wolter, S., Benndorf, D., Heyer, R., Hoffmann, M., Rapp, E., Bremges, A., Sczyrba, A., Schlüter, A., and Reichl, U. (2015). Fractionation of biogas plant sludge material improves metaproteomic characterization to investigate metabolic activity of microbial communities. Proteomics 15, 3585-3589.
Kohrs, F., et al., 2015. Fractionation of biogas plant sludge material improves metaproteomic characterization to investigate metabolic activity of microbial communities. Proteomics, 15(20), p 3585-3589.
F. Kohrs, et al., “Fractionation of biogas plant sludge material improves metaproteomic characterization to investigate metabolic activity of microbial communities”, Proteomics, vol. 15, 2015, pp. 3585-3589.
Kohrs, F., Wolter, S., Benndorf, D., Heyer, R., Hoffmann, M., Rapp, E., Bremges, A., Sczyrba, A., Schlüter, A., Reichl, U.: Fractionation of biogas plant sludge material improves metaproteomic characterization to investigate metabolic activity of microbial communities. Proteomics. 15, 3585-3589 (2015).
Kohrs, Fabian, Wolter, Sophie, Benndorf, Dirk, Heyer, Robert, Hoffmann, Marcus, Rapp, Erdmann, Bremges, Andreas, Sczyrba, Alexander, Schlüter, Andreas, and Reichl, Udo. “Fractionation of biogas plant sludge material improves metaproteomic characterization to investigate metabolic activity of microbial communities”. Proteomics 15.20 (2015): 3585-3589.

4 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Disseminating Metaproteomic Informatics Capabilities and Knowledge Using the Galaxy-P Framework.
Blank C, Easterly C, Gruening B, Johnson J, Kolmeder CA, Kumar P, May D, Mehta S, Mesuere B, Brown Z, Elias JE, Hervey WJ, McGowan T, Muth T, Nunn B, Rudney J, Tanca A, Griffin TJ, Jagtap PD., Proteomes 6(1), 2018
PMID: 29385081
Metabolic Adaptation of Methanogens in Anaerobic Digesters Upon Trace Element Limitation.
Wintsche B, Jehmlich N, Popp D, Harms H, Kleinsteuber S., Front Microbiol 9(), 2018
PMID: 29593674
Proteotyping of laboratory-scale biogas plants reveals multiple steady-states in community composition.
Kohrs F, Heyer R, Bissinger T, Kottler R, Schallert K, Püttker S, Behne A, Rapp E, Benndorf D, Reichl U., Anaerobe 46(), 2017
PMID: 28189830

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