Anaerobic digestion of the microalga Spirulina at extreme alkaline conditions: biogas production, metagenome, and metatranscriptome

Nolla Ardevol V, Strous M, Tegetmeyer H (2015)
Frontiers in Microbiology 6: 597.

Download
OA
Journal Article | Published | English
Abstract
A haloalkaline anaerobic microbial community obtained from soda lake sediments was used to inoculate anaerobic reactors for the production of methane rich biogas. The microalga Spirulina was successfully digested by the haloalkaline microbial consortium at alkaline conditions (pH 10, 2.0 M Na+). Continuous biogas production was observed and the obtained biogas was rich in methane, up to 96 %. Alkaline medium acted as a CO2 scrubber which resulted in low amounts of CO2 and no traces of H2S in the produced biogas. A hydraulic retention time of 15 days and 0.25 g Spirulina L-1 day-1 organic loading rate were identified as the optimal operational parameters. Metagenomics and metatranscriptomics analysis showed that the hydrolysis of the supplied substrate was mainly carried out by Bacteroidetes of the “ML635J-40 aquatic group” while the hydrogenotrophic pathway was the main producer of methane in a methanogenic community dominated by Methanocalculus.
Publishing Year
ISSN
Financial disclosure
Article Processing Charge funded by the Deutsche Forschungsgemeinschaft and the Open Access Publication Fund of Bielefeld University.
PUB-ID

Cite this

Nolla Ardevol V, Strous M, Tegetmeyer H. Anaerobic digestion of the microalga Spirulina at extreme alkaline conditions: biogas production, metagenome, and metatranscriptome. Frontiers in Microbiology. 2015;6: 597.
Nolla Ardevol, V., Strous, M., & Tegetmeyer, H. (2015). Anaerobic digestion of the microalga Spirulina at extreme alkaline conditions: biogas production, metagenome, and metatranscriptome. Frontiers in Microbiology, 6, 597. doi:10.3389/fmicb.2015.00597
Nolla Ardevol, V., Strous, M., and Tegetmeyer, H. (2015). Anaerobic digestion of the microalga Spirulina at extreme alkaline conditions: biogas production, metagenome, and metatranscriptome. Frontiers in Microbiology 6:597.
Nolla Ardevol, V., Strous, M., & Tegetmeyer, H., 2015. Anaerobic digestion of the microalga Spirulina at extreme alkaline conditions: biogas production, metagenome, and metatranscriptome. Frontiers in Microbiology, 6: 597.
V. Nolla Ardevol, M. Strous, and H. Tegetmeyer, “Anaerobic digestion of the microalga Spirulina at extreme alkaline conditions: biogas production, metagenome, and metatranscriptome”, Frontiers in Microbiology, vol. 6, 2015, : 597.
Nolla Ardevol, V., Strous, M., Tegetmeyer, H.: Anaerobic digestion of the microalga Spirulina at extreme alkaline conditions: biogas production, metagenome, and metatranscriptome. Frontiers in Microbiology. 6, : 597 (2015).
Nolla Ardevol, Vimac, Strous, Marc, and Tegetmeyer, Halina. “Anaerobic digestion of the microalga Spirulina at extreme alkaline conditions: biogas production, metagenome, and metatranscriptome”. Frontiers in Microbiology 6 (2015): 597.
Main File(s)
Access Level
OA Open Access
Last Uploaded
2016-11-28T15:05:19Z

This data publication is cited in the following publications:
This publication cites the following data publications:

1 Citation in Europe PMC

Data provided by Europe PubMed Central.

Ecology and application of haloalkaliphilic anaerobic microbial communities.
Sousa JA, Sorokin DY, Bijmans MF, Plugge CM, Stams AJ., Appl. Microbiol. Biotechnol. 99(22), 2015
PMID: 26359181

118 References

Data provided by Europe PubMed Central.

New perspectives in anaerobic digestion.
van Lier JB, Tilche A, Ahring BK, Macarie H, Moletta R, Dohanyos M, Pol LW, Lens P, Verstraete W; Management Committee of the IWA Anaerobic Digestion Specialised Group., Water Sci. Technol. 43(1), 2001
PMID: 11379079
Thermophilic and mesophilic methane production from anaerobic degradation of the cyanobacterium Spirulina maxima
Varel V., Chen T., Hashimoto A.., 1988
Unusual micro-organisms from unusual habitats: hypersaline environments
Ventosa A.., 2006
Anaerobic biodegradability and toxicity of wastewaters from chlorine and total chlorine-free bleaching of eucalyptus kraft pulps
Vidal G., Soto M., Field J., Méndez-Pampín R., Lema J.., 1997
Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy.
Wang Q, Garrity GM, Tiedje JM, Cole JR., Appl. Environ. Microbiol. 73(16), 2007
PMID: 17586664
Molecular analyses of microbial diversity associated with the Lonar soda lake in India: an impact crater in a basalt area.
Wani AA, Surakasi VP, Siddharth J, Raghavan RG, Patole MS, Ranade D, Shouche YS., Res. Microbiol. 157(10), 2006
PMID: 17070674
Anaerobic digestion of algae biomass: a review
Ward A., Lewis D., Green F.., 2014
Biogas production: current state and perspectives.
Weiland P., Appl. Microbiol. Biotechnol. 85(4), 2010
PMID: 19777226
Characterization of a biogas-producing microbial community by short-read next generation DNA sequencing.
Wirth R, Kovacs E, Maroti G, Bagi Z, Rakhely G, Kovacs KL., Biotechnol Biofuels 5(), 2012
PMID: 22673110
The roles of calcium in sludge granulation during UASB reactor start-up.
Yu HQ, Tay JH, Fang HH., Water Res. 35(4), 2001
PMID: 11235871
Bioenergetics of alkaliphilic Bacillus spp.
Yumoto I., J. Biosci. Bioeng. 93(4), 2002
PMID: 16233213
Influence of retention time on reactor performance and bacterial trophic populations in anaerobic digestion processes
Zhang T., Noike T.., 1994
How could haloalkaliphilic microorganisms contribute to biotechnology?
Zhao B, Yan Y, Chen S., Can. J. Microbiol. 60(11), 2014
PMID: 25372346
[Electron microscopy of the surfaces of bacillary spores].
Smirnova TA, Zubasheva MV, Shevliagina NV, Nikolaenko MA, Azizbekian RR., Mikrobiologiia 82(6), 2013
PMID: 25509408
Alkaliphilic anaerobic community at pH 10
Zhilina T., Zavarzin G.., 1994
DNA recovery from soils of diverse composition.
Zhou J, Bruns MA, Tiedje JM., Appl. Environ. Microbiol. 62(2), 1996
PMID: 8593035
Microbial community structure and dynamics during anaerobic digestion of various agricultural waste materials.
Ziganshin AM, Liebetrau J, Proter J, Kleinsteuber S., Appl. Microbiol. Biotechnol. 97(11), 2013
PMID: 23624683

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 26157422
PubMed | Europe PMC

Search this title in

Google Scholar