Corynebacterium glutamicum ATP-phosphoribosyl transferases suitable for L-histidine production - Strategies for the elimination of feedback inhibition

Kulis-Horn R, Persicke M, Kalinowski J (2015)
Journal of Biotechnology 206: 26-37.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Abstract / Bemerkung
L-Histidine biosynthesis in Corynebacterium glutamicum is mainly regulated by L-histidine feedback inhibition of the ATP-phosphoribosyltransferase HisG that catalyzes the first step of the pathway. The elimination of this feedback inhibition is the first and most important step in the development of an L-histidine production strain. For this purpose, a combined approach of random mutagenesis and rational enzyme redesign was performed. Mutants spontaneously resistant to the toxic L-histidine analog beta-(2-thiazolyl)-DL-alanine (2-TA) revealed novel and unpredicted mutations in the C-terminal regulatory domain of HisG resulting in increased feedback resistance. Moreover, deletion of the entire C-terminal regulatory domain in combination with the gain of function mutation S143F in the catalytic domain resulted in a HisG variant that is still highly active even at L-histidine concentrations close to the solubility limit. Notably, the S143F mutation on its own provokes feedback deregulation, revealing for the first time an amino acid residue in the catalytic domain of HisG that is involved in the feedback regulatory mechanism. In addition, we investigated the effect of hisG mutations for L-histidine production on different levels. This comprised the analysis of different expression systems, including plasmid- and chromosome-based overexpression, as well as the importance of codon choice for HisG mutations. The combination of domain deletions, single amino acid exchanges, codon choice, and chromosome-based overexpression resulted in production strains accumulating around 0.5 gl(-1) L-histidine, demonstrating the added value of the different approaches. (C) 2015 Published by Elsevier B.V.
Erscheinungsjahr
Zeitschriftentitel
Journal of Biotechnology
Band
206
Seite
26-37
ISSN
PUB-ID

Zitieren

Kulis-Horn R, Persicke M, Kalinowski J. Corynebacterium glutamicum ATP-phosphoribosyl transferases suitable for L-histidine production - Strategies for the elimination of feedback inhibition. Journal of Biotechnology. 2015;206:26-37.
Kulis-Horn, R., Persicke, M., & Kalinowski, J. (2015). Corynebacterium glutamicum ATP-phosphoribosyl transferases suitable for L-histidine production - Strategies for the elimination of feedback inhibition. Journal of Biotechnology, 206, 26-37. doi:10.1016/j.jbiotec.2015.04.001
Kulis-Horn, R., Persicke, M., and Kalinowski, J. (2015). Corynebacterium glutamicum ATP-phosphoribosyl transferases suitable for L-histidine production - Strategies for the elimination of feedback inhibition. Journal of Biotechnology 206, 26-37.
Kulis-Horn, R., Persicke, M., & Kalinowski, J., 2015. Corynebacterium glutamicum ATP-phosphoribosyl transferases suitable for L-histidine production - Strategies for the elimination of feedback inhibition. Journal of Biotechnology, 206, p 26-37.
R. Kulis-Horn, M. Persicke, and J. Kalinowski, “Corynebacterium glutamicum ATP-phosphoribosyl transferases suitable for L-histidine production - Strategies for the elimination of feedback inhibition”, Journal of Biotechnology, vol. 206, 2015, pp. 26-37.
Kulis-Horn, R., Persicke, M., Kalinowski, J.: Corynebacterium glutamicum ATP-phosphoribosyl transferases suitable for L-histidine production - Strategies for the elimination of feedback inhibition. Journal of Biotechnology. 206, 26-37 (2015).
Kulis-Horn, Robert, Persicke, Marcus, and Kalinowski, Jörn. “Corynebacterium glutamicum ATP-phosphoribosyl transferases suitable for L-histidine production - Strategies for the elimination of feedback inhibition”. Journal of Biotechnology 206 (2015): 26-37.

5 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

A dimeric catalytic core relates the short and long forms of ATP-phosphoribosyltransferase.
Mittelstädt G, Jiao W, Livingstone EK, Moggré GJ, Nazmi AR, Parker EJ., Biochem J 475(1), 2018
PMID: 29208762
Specific features of L-histidine production by Escherichia coli concerned with feedback control of AICAR formation and inorganic phosphate/metal transport.
Malykh EA, Butov IA, Ravcheeva AB, Krylov AA, Mashko SV, Stoynova NV., Microb Cell Fact 17(1), 2018
PMID: 29544475
Independent catalysis of the short form HisG from Lactococcus lactis.
Livingstone EK, Mittelstädt G, Given FM, Parker EJ., FEBS Lett 590(16), 2016
PMID: 27393206

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 25892668
PubMed | Europe PMC

Suchen in

Google Scholar