Genetic tools and techniques for Chlamydomonas reinhardtii

Mussgnug JH (2015)
Applied Microbiology and Biotechnology 99(13): 5407-5418.

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Journal Article | Published | English
Abstract
The development of tools has always been a major driving force for the advancement of science. Optical microscopes were the first instruments that allowed discovery and descriptive studies of the subcellular features of microorganisms. Although optical and electron microscopes remained at the forefront of microbiological research tools since their inventions, the advent of molecular genetics brought about questions which had to be addressed with new Bgenetic tools^. The unicellular green microalgal genus Chlamydomonas, especially the most prominent species C. reinhardtii, has become a frequently used model organism for many diverse fields of research and molecular genetic analyses of C. reinhardtii, as well as the available genetic tools and techniques, have become increasingly sophisticated throughout the last decades. The aim of this review is to provide an overview of the molecular key features of C. reinhardtii and summarize the progress related to the development of tools and techniques for genetic engineering of this organism, from pioneering DNA transformation experiments to state-of-theart techniques for targeted nuclear genome editing and highthroughput screening approaches.
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Mussgnug JH. Genetic tools and techniques for Chlamydomonas reinhardtii. Applied Microbiology and Biotechnology. 2015;99(13):5407-5418.
Mussgnug, J. H. (2015). Genetic tools and techniques for Chlamydomonas reinhardtii. Applied Microbiology and Biotechnology, 99(13), 5407-5418.
Mussgnug, J. H. (2015). Genetic tools and techniques for Chlamydomonas reinhardtii. Applied Microbiology and Biotechnology 99, 5407-5418.
Mussgnug, J.H., 2015. Genetic tools and techniques for Chlamydomonas reinhardtii. Applied Microbiology and Biotechnology, 99(13), p 5407-5418.
J.H. Mussgnug, “Genetic tools and techniques for Chlamydomonas reinhardtii”, Applied Microbiology and Biotechnology, vol. 99, 2015, pp. 5407-5418.
Mussgnug, J.H.: Genetic tools and techniques for Chlamydomonas reinhardtii. Applied Microbiology and Biotechnology. 99, 5407-5418 (2015).
Mussgnug, Jan H. “Genetic tools and techniques for Chlamydomonas reinhardtii”. Applied Microbiology and Biotechnology 99.13 (2015): 5407-5418.
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Experimental Genome-Wide Determination of RNA Polyadenylation in Chlamydomonas reinhardtii.
Bell SA, Shen C, Brown A, Hunt AG., PLoS ONE 11(1), 2016
PMID: 26730730

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