Engineering L-arabinose metabolism in triacylglycerol-producing Rhodococcus opacus for lignocellulosic fuel production

Kurosawa K, Plassmeier J, Kalinowski J, Rückert C, Sinskey AJ (2015)
Metabolic engineering 30: 89-95.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
: Advanced biofuels from lignocellulosic biomass have been considered as a potential solution for the issues of energy sustainability and environmental protection. Triacylglycerols (TAGs) are potential precursors for the production of lipid-based liquid biofuels. Rhodococcus opacus PD630 can accumulate large amounts of TAGs when grown under physiological conditions of high carbon and low nitrogen. However, R. opacus PD630 does not utilize the sugar L-arabinose present in lignocellulosic hydrolysates. Here, we report the engineering of R. opacus to produce TAGs on L-arabinose. We constructed a plasmid (pASC8057) harboring araB, araD and araA genes derived from a Streptomyces bacterium, and introduced the genes into R. opacus PD630. One of the engineered strains, MITAE-348, was capable of growing on high concentrations (up to 100g/L) of L-arabinose. MITAE-348 was grown in a defined medium containing 16g/L L-arabinose or a mixture of 8g/L L-arabinose and 8g/L D-glucose. In a stationary phase occurring 3 days post-inoculation, the strain was able to completely utilize the sugar, and yielded 2.0g/L for L-arabinose and 2.2g/L for L-arabinose/D-glucose of TAGs, corresponding to 39.7% or 42.0%, respectively, of the cell dry weight.
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Zeitschriftentitel
Metabolic engineering
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30
Seite
89-95
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Kurosawa K, Plassmeier J, Kalinowski J, Rückert C, Sinskey AJ. Engineering L-arabinose metabolism in triacylglycerol-producing Rhodococcus opacus for lignocellulosic fuel production. Metabolic engineering. 2015;30:89-95.
Kurosawa, K., Plassmeier, J., Kalinowski, J., Rückert, C., & Sinskey, A. J. (2015). Engineering L-arabinose metabolism in triacylglycerol-producing Rhodococcus opacus for lignocellulosic fuel production. Metabolic engineering, 30, 89-95. doi:10.1016/j.ymben.2015.04.006
Kurosawa, K., Plassmeier, J., Kalinowski, J., Rückert, C., and Sinskey, A. J. (2015). Engineering L-arabinose metabolism in triacylglycerol-producing Rhodococcus opacus for lignocellulosic fuel production. Metabolic engineering 30, 89-95.
Kurosawa, K., et al., 2015. Engineering L-arabinose metabolism in triacylglycerol-producing Rhodococcus opacus for lignocellulosic fuel production. Metabolic engineering, 30, p 89-95.
K. Kurosawa, et al., “Engineering L-arabinose metabolism in triacylglycerol-producing Rhodococcus opacus for lignocellulosic fuel production”, Metabolic engineering, vol. 30, 2015, pp. 89-95.
Kurosawa, K., Plassmeier, J., Kalinowski, J., Rückert, C., Sinskey, A.J.: Engineering L-arabinose metabolism in triacylglycerol-producing Rhodococcus opacus for lignocellulosic fuel production. Metabolic engineering. 30, 89-95 (2015).
Kurosawa, Kazuhiko, Plassmeier, Jens, Kalinowski, Jörn, Rückert, Christian, and Sinskey, Anthony J. “Engineering L-arabinose metabolism in triacylglycerol-producing Rhodococcus opacus for lignocellulosic fuel production”. Metabolic engineering 30 (2015): 89-95.

4 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Rapid metabolic analysis of Rhodococcus opacus PD630 via parallel 13C-metabolite fingerprinting.
Hollinshead WD, Henson WR, Abernathy M, Moon TS, Tang YJ., Biotechnol Bioeng 113(1), 2016
PMID: 26174624
Characterization of key triacylglycerol biosynthesis processes in rhodococci.
Amara S, Seghezzi N, Otani H, Diaz-Salazar C, Liu J, Eltis LD., Sci Rep 6(), 2016
PMID: 27126051
Engineering of an L-arabinose metabolic pathway in Rhodococcus jostii RHA1 for biofuel production.
Xiong X, Wang X, Chen S., J Ind Microbiol Biotechnol 43(7), 2016
PMID: 27143134
Rhodococcus opacus B4: a promising bacterium for production of biofuels and biobased chemicals.
Castro AR, Rocha I, Alves MM, Pereira MA., AMB Express 6(1), 2016
PMID: 27179529

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