Lysosomal integral membrane protein type-2 (LIMP-2/SCARB2) is a substrate of cathepsin-F, a cysteine protease mutated in type-B-Kufs-disease

Peters J, Rittger A, Weisner R, Knabbe J, Zunke F, Rothaug M, Damme M, Berkovic SF, Blanz J, Saftig P, Schwake M (2015)
Biochemical and Biophysical Research Communications 457(3): 334-340.

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DOI
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
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Einrichtung
Fakultät für Chemie > Biochemie III
Abstract / Bemerkung
The lysosomal integral membrane protein type-2 (LIMP-2/SCARB2) has been identified as a receptor for enterovirus 71 uptake and mannose-6-phosphate-independent lysosomal trafficking of the acid hydrolase beta-glucocerebrosidase. Here we show that LIMP-2 undergoes proteolytic cleavage mediated by lysosomal cysteine proteases. Heterologous expression and in vitro studies suggest that cathepsin-F is mainly responsible for the lysosomal processing of wild-type LIMP-2. Furthermore, examination of purified lysosomes revealed that LIMP-2 undergoes proteolysis in vivo. Mutations in the gene encoding cathepsin-F (CTSF) have recently been associated with type-B-Kufs-disease, an adult form of neuronal ceroid-lipofuscinosis. In this study we show that disease-causing cathepsin-F mutants fail to cleave LIMP-2. Our findings provide evidence that LIMP-2 represents an in vivo substrate of cathepsin-F with relevance for understanding the pathophysiology of type-B-Kufs-disease. (C) 2015 Elsevier Inc. All rights reserved.
Stichworte
ceroid-lipofuscinosis; Neuronal; Lysosomal storage disease; Cathepsin-F; LIMP-2; Kufs disease
Erscheinungsjahr
2015
Zeitschriftentitel
Biochemical and Biophysical Research Communications
Band
457
Zeitschriftennummer
3
Seite
334-340
ISSN
0006-291X
PUB-ID

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Peters J, Rittger A, Weisner R, et al. Lysosomal integral membrane protein type-2 (LIMP-2/SCARB2) is a substrate of cathepsin-F, a cysteine protease mutated in type-B-Kufs-disease. Biochemical and Biophysical Research Communications. 2015;457(3):334-340.
Peters, J., Rittger, A., Weisner, R., Knabbe, J., Zunke, F., Rothaug, M., Damme, M., et al. (2015). Lysosomal integral membrane protein type-2 (LIMP-2/SCARB2) is a substrate of cathepsin-F, a cysteine protease mutated in type-B-Kufs-disease. Biochemical and Biophysical Research Communications, 457(3), 334-340. doi:10.1016/j.bbrc.2014.12.111
Peters, J., Rittger, A., Weisner, R., Knabbe, J., Zunke, F., Rothaug, M., Damme, M., Berkovic, S. F., Blanz, J., Saftig, P., et al. (2015). Lysosomal integral membrane protein type-2 (LIMP-2/SCARB2) is a substrate of cathepsin-F, a cysteine protease mutated in type-B-Kufs-disease. Biochemical and Biophysical Research Communications 457, 334-340.
Peters, J., et al., 2015. Lysosomal integral membrane protein type-2 (LIMP-2/SCARB2) is a substrate of cathepsin-F, a cysteine protease mutated in type-B-Kufs-disease. Biochemical and Biophysical Research Communications, 457(3), p 334-340.
J. Peters, et al., “Lysosomal integral membrane protein type-2 (LIMP-2/SCARB2) is a substrate of cathepsin-F, a cysteine protease mutated in type-B-Kufs-disease”, Biochemical and Biophysical Research Communications, vol. 457, 2015, pp. 334-340.
Peters, J., Rittger, A., Weisner, R., Knabbe, J., Zunke, F., Rothaug, M., Damme, M., Berkovic, S.F., Blanz, J., Saftig, P., Schwake, M.: Lysosomal integral membrane protein type-2 (LIMP-2/SCARB2) is a substrate of cathepsin-F, a cysteine protease mutated in type-B-Kufs-disease. Biochemical and Biophysical Research Communications. 457, 334-340 (2015).
Peters, Judith, Rittger, Andrea, Weisner, Rebecca, Knabbe, Johannes, Zunke, Friederike, Rothaug, Michelle, Damme, Markus, Berkovic, Samuel F., Blanz, Judith, Saftig, Paul, and Schwake, Michael. “Lysosomal integral membrane protein type-2 (LIMP-2/SCARB2) is a substrate of cathepsin-F, a cysteine protease mutated in type-B-Kufs-disease”. Biochemical and Biophysical Research Communications 457.3 (2015): 334-340.

4 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Alu-Derived Alternative Splicing Events Specific to Macaca Lineages in CTSF Gene.
Lee JR, Park SJ, Kim YH, Choe SH, Cho HM, Lee SR, Kim SU, Kim JS, Sim BW, Song BS, Jeong KJ, Lee Y, Jin YB, Kang P, Huh JW, Chang KT., Mol Cells 40(2), 2017
PMID: 28196413
The Multifaceted Role of the Lysosomal Protease Cathepsins in Kidney Disease.
Cocchiaro P, De Pasquale V, Della Morte R, Tafuri S, Avallone L, Pizard A, Moles A, Pavone LM., Front Cell Dev Biol 5(), 2017
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Intramembrane proteolysis within lysosomes.
Schröder B, Saftig P., Ageing Res Rev 32(), 2016
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Mannose 6-phosphate-independent Lysosomal Sorting of LIMP-2.
Blanz J, Zunke F, Markmann S, Damme M, Braulke T, Saftig P, Schwake M., Traffic 16(10), 2015
PMID: 26219725

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