Fermentative production of the diamine putrescine: systems metabolic engineering of *Corynebacterium glutamicum*

Nguyen AQ, Komati Reddy G, Schneider J, Wendisch VF (2015)
Metabolites 5: 211-231.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
Abstract / Bemerkung
Corynebacterium glutamicum shows great potential for the production of the glutamate-derived diamine putrescine, a monomeric compound of polyamides. A genome-scale stoichiometric model of a C. glutamicum strain with reduced ornithine transcarbamoylase activity, derepressed arginine biosynthesis, and an anabolic plasmid-addiction system for heterologous expression of E. coli ornithine decarboxylase gene speC was investigated by flux balance analysis with respect to its putrescine production potential. Based on these simulations, enhancing glycolysis and anaplerosis by plasmid-borne overexpression of the genes for glyceraldehyde 3-phosphate dehydrogenase and pyruvate carboxylase as well as reducing 2-oxoglutarate dehydrogenase activity were chosen as targets for metabolic engineering. Changing the translational start codon of the chromosomal gene for 2-oxoglutarate dehydrogenase subunit E1o to the less preferred TTG and changing threonine 15 of OdhI to alanine reduced 2-oxoglutarate dehydrogenase activity about five fold and improved putrescine titers by 28%. Additional engineering steps improved further putrescine production with the largest contributions from preventing the formation of the by-product N-acetylputrescine by deletion of spermi(di)ne N-acetyltransferase gene snaA and from overexpression of the gene for a feedback-resistant N-acetylglutamate kinase variant. The resulting C. glutamicum strain NA6 obtained by systems metabolic engineering accumulated two fold more putrescine than the base strain, i.e., 58.1 ± 0.2 mM, and showed a specific productivity of 0.045 g·g−1·h−1 and a yield on glucose of 0.26 g·g−1.
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Zeitschriftentitel
Metabolites
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5
Seite
211-231
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Article Processing Charge funded by the Deutsche Forschungsgemeinschaft and the Open Access Publication Fund of Bielefeld University.
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Nguyen AQ, Komati Reddy G, Schneider J, Wendisch VF. Fermentative production of the diamine putrescine: systems metabolic engineering of *Corynebacterium glutamicum*. Metabolites. 2015;5:211-231.
Nguyen, A. Q., Komati Reddy, G., Schneider, J., & Wendisch, V. F. (2015). Fermentative production of the diamine putrescine: systems metabolic engineering of *Corynebacterium glutamicum*. Metabolites, 5, 211-231. doi:10.3390/metabo5020211
Nguyen, A. Q., Komati Reddy, G., Schneider, J., and Wendisch, V. F. (2015). Fermentative production of the diamine putrescine: systems metabolic engineering of *Corynebacterium glutamicum*. Metabolites 5, 211-231.
Nguyen, A.Q., et al., 2015. Fermentative production of the diamine putrescine: systems metabolic engineering of *Corynebacterium glutamicum*. Metabolites, 5, p 211-231.
A.Q. Nguyen, et al., “Fermentative production of the diamine putrescine: systems metabolic engineering of *Corynebacterium glutamicum*”, Metabolites, vol. 5, 2015, pp. 211-231.
Nguyen, A.Q., Komati Reddy, G., Schneider, J., Wendisch, V.F.: Fermentative production of the diamine putrescine: systems metabolic engineering of *Corynebacterium glutamicum*. Metabolites. 5, 211-231 (2015).
Nguyen, Anh Quynh, Komati Reddy, Gajendar, Schneider, Jens, and Wendisch, Volker F. “Fermentative production of the diamine putrescine: systems metabolic engineering of *Corynebacterium glutamicum*”. Metabolites 5 (2015): 211-231.
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2016-11-28T14:29:02Z

10 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Engineering tunable biosensors for monitoring putrescine in Escherichia coli.
Chen XF, Xia XX, Lee SY, Qian ZG., Biotechnol Bioeng 115(4), 2018
PMID: 29251347
Metabolic evolution and a comparative omics analysis of Corynebacterium glutamicum for putrescine production.
Li Z, Shen YP, Jiang XL, Feng LS, Liu JZ., J Ind Microbiol Biotechnol 45(2), 2018
PMID: 29344811
Biotechnological production of mono- and diamines using bacteria: recent progress, applications, and perspectives.
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PMID: 29520601
Systems metabolic engineering strategies for the production of amino acids.
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PMID: 29062965
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Zhang B, Yu M, Zhou Y, Li Y, Ye BC., Microb Cell Fact 16(1), 2017
PMID: 28938890
Engineering cell factories for producing building block chemicals for bio-polymer synthesis.
Tsuge Y, Kawaguchi H, Sasaki K, Kondo A., Microb Cell Fact 15(), 2016
PMID: 26794242
Recent advances in amino acid production by microbial cells.
Hirasawa T, Shimizu H., Curr Opin Biotechnol 42(), 2016
PMID: 27151315

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