Slow-freezing versus vitrification for human ovarian tissue cryopreservation

Klocke S, Buendgen N, Koester F, Eichenlaub-Ritter U, Griesinger G (2015)
Archives of Gynecology and Obstetrics 291(2): 419-426.

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Ovarian tissue can be cryopreserved prior to chemotherapy using either the slow-freezing or the vitrification method; however, the data on the equality of the procedures are still conflicting. In this study, a comparison of the cryo-damage of human ovarian tissue induced by either vitrification or slow-freezing was performed. Ovarian tissue from 23 pre-menopausal patients was cryopreserved with either slow-freezing or vitrification. After thawing/warming, the tissue was histologically and immunohistochemically analyzed and cultured in vitro. During tissue culture the estradiol release was assessed. No significant difference was found in the proportion of high-quality follicles after thawing/warming in the slow-freezing and vitrification group, respectively (72.7 versus 66.7 %, p = 0.733). Estradiol secretion by the ovarian tissue was similar between groups during 18 days in vitro culture (area-under-the-curve 5,411 versus 13,102, p = 0.11). Addition of Sphingosine-1-Phosphate or Activin A to the culture medium did not alter estradiol release in both groups. The proportion of Activated Caspase-3 or 'Proliferating-Cell-Nuclear-Antigen' positive follicles at the end of the culture period was similar between slow-freezing and vitrification. Slow-freezing and vitrification result in similar morphological integrity after cryopreservation, a similar estradiol release in culture, and similar rates of follicular proliferation and apoptosis after culture.
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Klocke S, Buendgen N, Koester F, Eichenlaub-Ritter U, Griesinger G. Slow-freezing versus vitrification for human ovarian tissue cryopreservation. Archives of Gynecology and Obstetrics. 2015;291(2):419-426.
Klocke, S., Buendgen, N., Koester, F., Eichenlaub-Ritter, U., & Griesinger, G. (2015). Slow-freezing versus vitrification for human ovarian tissue cryopreservation. Archives of Gynecology and Obstetrics, 291(2), 419-426. doi:10.1007/s00404-014-3390-6
Klocke, S., Buendgen, N., Koester, F., Eichenlaub-Ritter, U., and Griesinger, G. (2015). Slow-freezing versus vitrification for human ovarian tissue cryopreservation. Archives of Gynecology and Obstetrics 291, 419-426.
Klocke, S., et al., 2015. Slow-freezing versus vitrification for human ovarian tissue cryopreservation. Archives of Gynecology and Obstetrics, 291(2), p 419-426.
S. Klocke, et al., “Slow-freezing versus vitrification for human ovarian tissue cryopreservation”, Archives of Gynecology and Obstetrics, vol. 291, 2015, pp. 419-426.
Klocke, S., Buendgen, N., Koester, F., Eichenlaub-Ritter, U., Griesinger, G.: Slow-freezing versus vitrification for human ovarian tissue cryopreservation. Archives of Gynecology and Obstetrics. 291, 419-426 (2015).
Klocke, Silke, Buendgen, Nana, Koester, Frank, Eichenlaub-Ritter, Ursula, and Griesinger, Georg. “Slow-freezing versus vitrification for human ovarian tissue cryopreservation”. Archives of Gynecology and Obstetrics 291.2 (2015): 419-426.
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Henry L, Fransolet M, Labied S, Blacher S, Masereel MC, Foidart JM, Noel A, Nisolle M, Munaut C., J Ovarian Res 9(), 2016
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