The DNA methylation landscape of Chinese hamster ovary (CHO) DP-12 cells

Wippermann A, Rupp O, Brinkrolf K, Hoffrogge R, Noll T (2015)
Journal of Biotechnology 199: 38-46.

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Abstract / Notes
Chinese hamster ovary (CHO) cells represent the most commonly used production cell line for therapeutic proteins. By recent genome and transcriptome sequencing a basis was created for future investigations of genotype-phenotype relationships and for improvement of CHO cell productivity and product quality. In this context information is missing about DNA cytosine methylation as a crucial epigenetic modification and an important element in mammalian genome regulation and development. Here, we present the first DNA methylation map of a CHO cell line in single-base resolution that was generated by whole genome bisulfite sequencing combined with gene expression analysis by CHO microarrays. We show CHO DP-12 cells to exhibit global hypomethylation compared to a majority of mammalian methylomes and hypermethylation of CpG-dense regions at gene promoters called CpG islands. We also observed partially methylated domains that cover 62% of the CHO DP-12 cell genome and contain functional clusters of genes. Gene expression analysis showed these clusters to be either highly or weakly expressed with regard to CHO-specific characteristics and hence proves DNA methylation in CHO cells to be an important link between genomics and transcriptomics.
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Wippermann A, Rupp O, Brinkrolf K, Hoffrogge R, Noll T. The DNA methylation landscape of Chinese hamster ovary (CHO) DP-12 cells. Journal of Biotechnology. 2015;199:38-46.
Wippermann, A., Rupp, O., Brinkrolf, K., Hoffrogge, R., & Noll, T. (2015). The DNA methylation landscape of Chinese hamster ovary (CHO) DP-12 cells. Journal of Biotechnology, 199, 38-46. doi:10.1016/j.jbiotec.2015.01.014
Wippermann, A., Rupp, O., Brinkrolf, K., Hoffrogge, R., and Noll, T. (2015). The DNA methylation landscape of Chinese hamster ovary (CHO) DP-12 cells. Journal of Biotechnology 199, 38-46.
Wippermann, A., et al., 2015. The DNA methylation landscape of Chinese hamster ovary (CHO) DP-12 cells. Journal of Biotechnology, 199, p 38-46.
A. Wippermann, et al., “The DNA methylation landscape of Chinese hamster ovary (CHO) DP-12 cells”, Journal of Biotechnology, vol. 199, 2015, pp. 38-46.
Wippermann, A., Rupp, O., Brinkrolf, K., Hoffrogge, R., Noll, T.: The DNA methylation landscape of Chinese hamster ovary (CHO) DP-12 cells. Journal of Biotechnology. 199, 38-46 (2015).
Wippermann, Anna, Rupp, Oliver, Brinkrolf, Karina, Hoffrogge, Raimund, and Noll, Thomas. “The DNA methylation landscape of Chinese hamster ovary (CHO) DP-12 cells”. Journal of Biotechnology 199 (2015): 38-46.
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8 Citations in Europe PMC

Data provided by Europe PubMed Central.

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Feichtinger J, Hernández I, Fischer C, Hanscho M, Auer N, Hackl M, Jadhav V, Baumann M, Krempl PM, Schmidl C, Farlik M, Schuster M, Merkel A, Sommer A, Heath S, Rico D, Bock C, Thallinger GG, Borth N., Biotechnol Bioeng 113(10), 2016
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Moritz B, Becker PB, Göpfert U., Sci Rep 5(), 2015
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