Carbon source dependent biosynthesis of acarviose metabolites in Actinoplanes sp SE50/110

Wendler S, Ortseifen V, Persicke M, Klein A, Neshat A, Niehaus K, Schneiker-Bekel S, Walter F, Wehmeier UF, Kalinowski J, Pühler A (2014)
Journal of Biotechnology 191: 113-120.

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Abstract
In this work the biosynthesis of the type 2 diabetes mellitus therapeutic acarviosyl-maltose (acarbose) and related acarviose metabolites produced by Actinoplanes sp. SE50/110 was studied in liquid minimal medium supplemented with the defined carbon sources maltose, glucose, galactose or mixtures of maltose/glucose and maltose/galactose. Quantifying acarviosyl-maltose by HPLC and UV detection revealed that only cultures grown in maltose-containing minimal media produced acarviosyl-maltose in significant amounts. A qualitative analysis of the cytosolic and extracellular proteome for the presence of proteins from the acarbose biosynthesis gene cluster showed that these were not only synthesizedin maltose-containing media, but also in media with glucose or galactose as the sole carbon source. A LC-MS-based detection method was applied to test the hypothesis that different acarviose metabolites are produced in media with maltose, glucose or galactose. The analysis revealed that a spectrum of acarviose metabolites (acarviose with 1-4 glucose equivalent units) was formed under all tested conditions. As expected, in maltose-containing minimal media acarviosyl-maltose was produced as the major component exceeding the remaining minor components by 2-3 orders of magnitude. In minimal medium supplemented with glucose acarviosyl-glucose was the major component, while in minimal medium with galactose no major component was present. Based on the results presented, a model for the intracellular biosynthesis of major and minor acarviose metabolites was developed. (C) 2014 Elsevier B.V. All rights reserved.
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Wendler S, Ortseifen V, Persicke M, et al. Carbon source dependent biosynthesis of acarviose metabolites in Actinoplanes sp SE50/110. Journal of Biotechnology. 2014;191:113-120.
Wendler, S., Ortseifen, V., Persicke, M., Klein, A., Neshat, A., Niehaus, K., Schneiker-Bekel, S., et al. (2014). Carbon source dependent biosynthesis of acarviose metabolites in Actinoplanes sp SE50/110. Journal of Biotechnology, 191, 113-120.
Wendler, S., Ortseifen, V., Persicke, M., Klein, A., Neshat, A., Niehaus, K., Schneiker-Bekel, S., Walter, F., Wehmeier, U. F., Kalinowski, J., et al. (2014). Carbon source dependent biosynthesis of acarviose metabolites in Actinoplanes sp SE50/110. Journal of Biotechnology 191, 113-120.
Wendler, S., et al., 2014. Carbon source dependent biosynthesis of acarviose metabolites in Actinoplanes sp SE50/110. Journal of Biotechnology, 191, p 113-120.
S. Wendler, et al., “Carbon source dependent biosynthesis of acarviose metabolites in Actinoplanes sp SE50/110”, Journal of Biotechnology, vol. 191, 2014, pp. 113-120.
Wendler, S., Ortseifen, V., Persicke, M., Klein, A., Neshat, A., Niehaus, K., Schneiker-Bekel, S., Walter, F., Wehmeier, U.F., Kalinowski, J., Pühler, A.: Carbon source dependent biosynthesis of acarviose metabolites in Actinoplanes sp SE50/110. Journal of Biotechnology. 191, 113-120 (2014).
Wendler, Sergej, Ortseifen, Vera, Persicke, Marcus, Klein, Andreas, Neshat, Armin, Niehaus, Karsten, Schneiker-Bekel, Susanne, Walter, Frederik, Wehmeier, Udo F., Kalinowski, Jörn, and Pühler, Alfred. “Carbon source dependent biosynthesis of acarviose metabolites in Actinoplanes sp SE50/110”. Journal of Biotechnology 191 (2014): 113-120.
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1 Citation in Europe PMC

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