Methanol-based cadaverine production by genetically engineered *Bacillus methanolicus* strains

Naerdal I, Pfeifenschneider J, Brautaset T, Wendisch VF (2015)
Microbial Biotechnology 8(2): 342-350.

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Summary Methanol is regarded as an attractive substrate for biotechnological production of value-added bulk products, such as amino acids and polyamines. In the present study, the methylotrophic and thermophilic bacterium Bacillus methanolicus was engineered into a microbial cell factory for the production of the platform chemical 1,5-diaminopentane (cadaverine) from methanol. This was achieved by the heterologous expression of the Escherichia coli genes cadA and ldcC encoding two different lysine decarboxylase enzymes, and by increasing the overall L-lysine production levels in this host. Both CadA and LdcC were functional in B. methanolicus cultivated at 50°C and expression of cadA resulted in cadaverine production levels up to 500 mg l−1 during shake flask conditions. A volume-corrected concentration of 11.3 g l−1 of cadaverine was obtained by high-cell density fed-batch methanol fermentation. Our results demonstrated that efficient conversion of L-lysine into cadaverine presumably has severe effects on feedback regulation of the L-lysine biosynthetic pathway in B. methanolicus. By also investigating the cadaverine tolerance level, B. methanolicus proved to be an exciting alternative host and comparable to the well-known bacterial hosts E. coli and Corynebacterium glutamicum. This study represents the first demonstration of microbial production of cadaverine from methanol.
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Naerdal I, Pfeifenschneider J, Brautaset T, Wendisch VF. Methanol-based cadaverine production by genetically engineered *Bacillus methanolicus* strains. Microbial Biotechnology. 2015;8(2):342-350.
Naerdal, I., Pfeifenschneider, J., Brautaset, T., & Wendisch, V. F. (2015). Methanol-based cadaverine production by genetically engineered *Bacillus methanolicus* strains. Microbial Biotechnology, 8(2), 342-350.
Naerdal, I., Pfeifenschneider, J., Brautaset, T., and Wendisch, V. F. (2015). Methanol-based cadaverine production by genetically engineered *Bacillus methanolicus* strains. Microbial Biotechnology 8, 342-350.
Naerdal, I., et al., 2015. Methanol-based cadaverine production by genetically engineered *Bacillus methanolicus* strains. Microbial Biotechnology, 8(2), p 342-350.
I. Naerdal, et al., “Methanol-based cadaverine production by genetically engineered *Bacillus methanolicus* strains”, Microbial Biotechnology, vol. 8, 2015, pp. 342-350.
Naerdal, I., Pfeifenschneider, J., Brautaset, T., Wendisch, V.F.: Methanol-based cadaverine production by genetically engineered *Bacillus methanolicus* strains. Microbial Biotechnology. 8, 342-350 (2015).
Naerdal, Ingemar, Pfeifenschneider, Johannes, Brautaset, Trygve, and Wendisch, Volker F. “Methanol-based cadaverine production by genetically engineered *Bacillus methanolicus* strains”. Microbial Biotechnology 8.2 (2015): 342-350.
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