Single-molecule quantification of lipotoxic expression of activating transcription factor 3

Yahiatene I, Aung HH, Wilson DW, Rutledge JC (2014)
Physical Chemistry Chemical Physics 16(39): 21595-21601.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ;
Abstract
Activating transcription factor 3 (ATF3) is a member of the mammalian activation transcription factor/cAMP, physiologically important in the regulation of pro- and anti-inflammatory target genes. We compared the induction of ATF3 protein as measured by Western blot analysis with single-molecule localization microscopy dSTORM to quantify the dynamics of accumulation of intranuclear ATF3 of triglyceride-rich (TGRL) lipolysis product-treated HAEC (Human Aortic Endothelial Cells). The ATF3 expression rate within the first three hours after treatment with TGRL lipolysis products is about 3500 h(-1). After three hours we detected 33 090 3491 single-molecule localizations of ATF3. This was accompanied by significant structural changes in the F-actin network of the cells at similar to 3-fold increased localization precision compared to widefield microscopy after treatment. Additionally, we discovered a cluster size of approximately 384 nanometers of ATF3 molecules. We show for the first time the time course of ATF3 accumulation in the nucleus undergoing lipotoxic injury. Furthermore, we demonstrate ATF3 accumulation associated with increased concentrations of TGRL lipotysis products occurs in large aggregates.
Publishing Year
ISSN
PUB-ID

Cite this

Yahiatene I, Aung HH, Wilson DW, Rutledge JC. Single-molecule quantification of lipotoxic expression of activating transcription factor 3. Physical Chemistry Chemical Physics. 2014;16(39):21595-21601.
Yahiatene, I., Aung, H. H., Wilson, D. W., & Rutledge, J. C. (2014). Single-molecule quantification of lipotoxic expression of activating transcription factor 3. Physical Chemistry Chemical Physics, 16(39), 21595-21601.
Yahiatene, I., Aung, H. H., Wilson, D. W., and Rutledge, J. C. (2014). Single-molecule quantification of lipotoxic expression of activating transcription factor 3. Physical Chemistry Chemical Physics 16, 21595-21601.
Yahiatene, I., et al., 2014. Single-molecule quantification of lipotoxic expression of activating transcription factor 3. Physical Chemistry Chemical Physics, 16(39), p 21595-21601.
I. Yahiatene, et al., “Single-molecule quantification of lipotoxic expression of activating transcription factor 3”, Physical Chemistry Chemical Physics, vol. 16, 2014, pp. 21595-21601.
Yahiatene, I., Aung, H.H., Wilson, D.W., Rutledge, J.C.: Single-molecule quantification of lipotoxic expression of activating transcription factor 3. Physical Chemistry Chemical Physics. 16, 21595-21601 (2014).
Yahiatene, Idir, Aung, Hnin H., Wilson, Dennis W., and Rutledge, John C. “Single-molecule quantification of lipotoxic expression of activating transcription factor 3”. Physical Chemistry Chemical Physics 16.39 (2014): 21595-21601.
This data publication is cited in the following publications:
This publication cites the following data publications:

31 References

Data provided by Europe PubMed Central.


Yahiatene Idir, Hennig Simon, Huser Thomas., 2013

Henriques R, Lelek M, Fornasiero EF., 2010

Sebastian M., Barbara Müller, Hans-Georg Krausslich, Mike Heilemann., 2013
ATF3 transcription factor and its emerging roles in immunity and cancer.
Thompson MR, Xu D, Williams BR., J. Mol. Med. 87(11), 2009
PMID: 19705082

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 25189785
PubMed | Europe PMC

Search this title in

Google Scholar