L-citrulline production by metabolically engineered *Corynebacterium glutamicum* from glucose and alternative carbon sources

Eberhardt D, Vold Korgaard Jensen J, Wendisch VF (2014)
AMB Express 4(85).

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Journal Article | Published | English
Abstract
L-citrulline plays an important role in human health and nutrition and is an intermediate of the L-arginine biosynthetic pathway. L-citrulline is a by-product of L-arginine production by Corynebacterium glutamicum. In this study, C. glutamicum was engineered for overproduction of L-citrulline as major product without L-arginine being produced as by-product. To this end, L-arginine biosynthesis was derepressed by deletion of the arginine repressor gene argR and conversion of L-citrulline towards L-arginine was avoided by deletion of the argininosuccinate synthetase gene argG. Moreover, to facilitate L-citrulline production the gene encoding a feedback resistant N-acetyl L-glutamate kinase argBfbr as well as the gene encoding L-ornithine carbamoylphosphate transferase argF were overexpressed. The resulting strain accumulated 44.1 ± 0.5 mM L-citrulline from glucose minimal medium with a yield of 0.38 ± 0.01 g⋅g−1 and a volumetric productivity of 0.32 ± 0.01 g⋅l−1⋅h−1. In addition, production of L-citrulline from the alternative carbon sources starch, xylose, and glucosamine could be demonstrated.
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Eberhardt D, Vold Korgaard Jensen J, Wendisch VF. L-citrulline production by metabolically engineered *Corynebacterium glutamicum* from glucose and alternative carbon sources. AMB Express. 2014;4(85).
Eberhardt, D., Vold Korgaard Jensen, J., & Wendisch, V. F. (2014). L-citrulline production by metabolically engineered *Corynebacterium glutamicum* from glucose and alternative carbon sources. AMB Express, 4(85).
Eberhardt, D., Vold Korgaard Jensen, J., and Wendisch, V. F. (2014). L-citrulline production by metabolically engineered *Corynebacterium glutamicum* from glucose and alternative carbon sources. AMB Express 4.
Eberhardt, D., Vold Korgaard Jensen, J., & Wendisch, V.F., 2014. L-citrulline production by metabolically engineered *Corynebacterium glutamicum* from glucose and alternative carbon sources. AMB Express, 4(85).
D. Eberhardt, J. Vold Korgaard Jensen, and V.F. Wendisch, “L-citrulline production by metabolically engineered *Corynebacterium glutamicum* from glucose and alternative carbon sources”, AMB Express, vol. 4, 2014.
Eberhardt, D., Vold Korgaard Jensen, J., Wendisch, V.F.: L-citrulline production by metabolically engineered *Corynebacterium glutamicum* from glucose and alternative carbon sources. AMB Express. 4, (2014).
Eberhardt, Dorit, Vold Korgaard Jensen, Jaide, and Wendisch, Volker F. “L-citrulline production by metabolically engineered *Corynebacterium glutamicum* from glucose and alternative carbon sources”. AMB Express 4.85 (2014).
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PMID: 26267114
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