L-citrulline production by metabolically engineered *Corynebacterium glutamicum* from glucose and alternative carbon sources

Eberhardt D, Vold Korgaard Jensen J, Wendisch VF (2014)
AMB Express 4(85).

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Abstract / Bemerkung
L-citrulline plays an important role in human health and nutrition and is an intermediate of the L-arginine biosynthetic pathway. L-citrulline is a by-product of L-arginine production by Corynebacterium glutamicum. In this study, C. glutamicum was engineered for overproduction of L-citrulline as major product without L-arginine being produced as by-product. To this end, L-arginine biosynthesis was derepressed by deletion of the arginine repressor gene argR and conversion of L-citrulline towards L-arginine was avoided by deletion of the argininosuccinate synthetase gene argG. Moreover, to facilitate L-citrulline production the gene encoding a feedback resistant N-acetyl L-glutamate kinase argBfbr as well as the gene encoding L-ornithine carbamoylphosphate transferase argF were overexpressed. The resulting strain accumulated 44.1 ± 0.5 mM L-citrulline from glucose minimal medium with a yield of 0.38 ± 0.01 g⋅g−1 and a volumetric productivity of 0.32 ± 0.01 g⋅l−1⋅h−1. In addition, production of L-citrulline from the alternative carbon sources starch, xylose, and glucosamine could be demonstrated.
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AMB Express
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4
Zeitschriftennummer
85
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Eberhardt D, Vold Korgaard Jensen J, Wendisch VF. L-citrulline production by metabolically engineered *Corynebacterium glutamicum* from glucose and alternative carbon sources. AMB Express. 2014;4(85).
Eberhardt, D., Vold Korgaard Jensen, J., & Wendisch, V. F. (2014). L-citrulline production by metabolically engineered *Corynebacterium glutamicum* from glucose and alternative carbon sources. AMB Express, 4(85). doi:10.1186/s13568-014-0085-0
Eberhardt, D., Vold Korgaard Jensen, J., and Wendisch, V. F. (2014). L-citrulline production by metabolically engineered *Corynebacterium glutamicum* from glucose and alternative carbon sources. AMB Express 4.
Eberhardt, D., Vold Korgaard Jensen, J., & Wendisch, V.F., 2014. L-citrulline production by metabolically engineered *Corynebacterium glutamicum* from glucose and alternative carbon sources. AMB Express, 4(85).
D. Eberhardt, J. Vold Korgaard Jensen, and V.F. Wendisch, “L-citrulline production by metabolically engineered *Corynebacterium glutamicum* from glucose and alternative carbon sources”, AMB Express, vol. 4, 2014.
Eberhardt, D., Vold Korgaard Jensen, J., Wendisch, V.F.: L-citrulline production by metabolically engineered *Corynebacterium glutamicum* from glucose and alternative carbon sources. AMB Express. 4, (2014).
Eberhardt, Dorit, Vold Korgaard Jensen, Jaide, and Wendisch, Volker F. “L-citrulline production by metabolically engineered *Corynebacterium glutamicum* from glucose and alternative carbon sources”. AMB Express 4.85 (2014).
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2015-12-11T13:48:19Z

11 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

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Pérez-García F, Max Risse J, Friehs K, Wendisch VF., Biotechnol J 12(7), 2017
PMID: 28169491
Production of amino acids - Genetic and metabolic engineering approaches.
Lee JH, Wendisch VF., Bioresour Technol 245(pt b), 2017
PMID: 28552565
Citrulline metabolism in plants.
Joshi V, Fernie AR., Amino Acids 49(9), 2017
PMID: 28741223
Insight of Genus Corynebacterium: Ascertaining the Role of Pathogenic and Non-pathogenic Species.
Oliveira A, Oliveira LC, Aburjaile F, Benevides L, Tiwari S, Jamal SB, Silva A, Figueiredo HCP, Ghosh P, Portela RW, De Carvalho Azevedo VA, Wattam AR., Front Microbiol 8(), 2017
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Updates on industrial production of amino acids using Corynebacterium glutamicum.
Wendisch VF, Jorge JMP, Pérez-García F, Sgobba E., World J Microbiol Biotechnol 32(6), 2016
PMID: 27116971
Roles of export genes cgmA and lysE for the production of L-arginine and L-citrulline by Corynebacterium glutamicum.
Lubitz D, Jorge JM, Pérez-García F, Taniguchi H, Wendisch VF., Appl Microbiol Biotechnol 100(19), 2016
PMID: 27350619

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