Methylotrophy in the thermophilic *Bacillus methanolicus*, basic insights and application for commodity productions from methanol

Müller JEN, Heggeset TMB, Wendisch VF, Vorholt JA, Brautaset T (2015)
Applied Microbiology and Biotechnology 99(2): 535-551.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ;
Abstract
Using methanol as an alternative non-food feedstock for biotechnological production offers several advantages in line with a methanol-based bioeconomy. The Gram-positive, facultative methylotrophic and thermophilic bacterium Bacillus methanolicus is one of the few described microbial candidates with a potential for the conversion of methanol to value-added products. Its capabilities of producing and secreting the commercially important amino acids l-glutamate and l-lysine to high concentrations at 50 A degrees C have been demonstrated and make B. methanolicus a promising target to develop cell factories for industrial-scale production processes. B. methanolicus uses the ribulose monophosphate cycle for methanol assimilation and represents the first example of plasmid-dependent methylotrophy. Recent genome sequencing of two physiologically different wild-type B. methanolicus strains, MGA3 and PB1, accompanied with transcriptome and proteome analyses has generated fundamental new insight into the metabolism of the species. In addition, multiple key enzymes representing methylotrophic and biosynthetic pathways have been biochemically characterized. All this, together with establishment of improved tools for gene expression, has opened opportunities for systems-level metabolic engineering of B. methanolicus. Here, we summarize the current status of its metabolism and biochemistry, available genetic tools, and its potential use in respect to overproduction of amino acids.
Publishing Year
ISSN
PUB-ID

Cite this

Müller JEN, Heggeset TMB, Wendisch VF, Vorholt JA, Brautaset T. Methylotrophy in the thermophilic *Bacillus methanolicus*, basic insights and application for commodity productions from methanol. Applied Microbiology and Biotechnology. 2015;99(2):535-551.
Müller, J. E. N., Heggeset, T. M. B., Wendisch, V. F., Vorholt, J. A., & Brautaset, T. (2015). Methylotrophy in the thermophilic *Bacillus methanolicus*, basic insights and application for commodity productions from methanol. Applied Microbiology and Biotechnology, 99(2), 535-551.
Müller, J. E. N., Heggeset, T. M. B., Wendisch, V. F., Vorholt, J. A., and Brautaset, T. (2015). Methylotrophy in the thermophilic *Bacillus methanolicus*, basic insights and application for commodity productions from methanol. Applied Microbiology and Biotechnology 99, 535-551.
Müller, J.E.N., et al., 2015. Methylotrophy in the thermophilic *Bacillus methanolicus*, basic insights and application for commodity productions from methanol. Applied Microbiology and Biotechnology, 99(2), p 535-551.
J.E.N. Müller, et al., “Methylotrophy in the thermophilic *Bacillus methanolicus*, basic insights and application for commodity productions from methanol”, Applied Microbiology and Biotechnology, vol. 99, 2015, pp. 535-551.
Müller, J.E.N., Heggeset, T.M.B., Wendisch, V.F., Vorholt, J.A., Brautaset, T.: Methylotrophy in the thermophilic *Bacillus methanolicus*, basic insights and application for commodity productions from methanol. Applied Microbiology and Biotechnology. 99, 535-551 (2015).
Müller, Jonas E. N., Heggeset, Tonje M. B., Wendisch, Volker F., Vorholt, Julia A., and Brautaset, Trygve. “Methylotrophy in the thermophilic *Bacillus methanolicus*, basic insights and application for commodity productions from methanol”. Applied Microbiology and Biotechnology 99.2 (2015): 535-551.
This data publication is cited in the following publications:
This publication cites the following data publications:

0 Citations in Europe PMC

Data provided by Europe PubMed Central.

70 References

Data provided by Europe PubMed Central.

Pyruvate carboxylase is a major bottleneck for glutamate and lysine production by Corynebacterium glutamicum.
Peters-Wendisch PG, Schiel B, Wendisch VF, Katsoulidis E, Mockel B, Sahm H, Eikmanns BJ., J. Mol. Microbiol. Biotechnol. 3(2), 2001
PMID: 11321586

AUTHOR UNKNOWN, 0

AUTHOR UNKNOWN, 0
Methanol-based industrial biotechnology: current status and future perspectives of methylotrophic bacteria.
Schrader J, Schilling M, Holtmann D, Sell D, Filho MV, Marx A, Vorholt JA., Trends Biotechnol. 27(2), 2009
PMID: 19111927
The methylotrophic Bacillus methanolicus MGA3 possesses two distinct fructose 1,6-bisphosphate aldolases.
Stolzenberger J, Lindner SN, Wendisch VF., Microbiology (Reading, Engl.) 159(Pt 8), 2013
PMID: 23760818

SR, 1975

AUTHOR UNKNOWN, 0
Electron microscopic analysis and biochemical characterization of a novel methanol dehydrogenase from the thermotolerant Bacillus sp. C1.
Vonck J, Arfman N, De Vries GE, Van Beeumen J, Van Bruggen EF, Dijkhuizen L., J. Biol. Chem. 266(6), 1991
PMID: 1995642

VF, Curr Opin Biotechnol 30C(), 2014
Double deletion of dtsR1 and pyc induce efficient L: -glutamate overproduction in Corynebacterium glutamicum.
Yao W, Deng X, Zhong H, Liu M, Zheng P, Sun Z, Zhang Y., J. Ind. Microbiol. Biotechnol. 36(7), 2009
PMID: 19408028

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 25431011
PubMed | Europe PMC

Search this title in

Google Scholar