Induced Neural Stem Cells Achieve Long-Term Survival and Functional Integration in the Adult Mouse Brain

Hemmer K, Zhang M, van Wuellen T, Sakalem M, Tapia N, Baumuratov A, Kaltschmidt C, Kaltschmidt B, Schoeler HR, Zhang W, Schwamborn JC (2014)
Stem Cell Reports 3(3): 423-431.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
Differentiated cells can be converted directly into multipotent neural stem cells (i.e., induced neural stem cells [iNSCs]). iNSCs offer an attractive alternative to induced pluripotent stem cell (iPSC) technology with regard to regenerative therapies. Here, we show an in vivo long-term analysis of transplanted iNSCs in the adult mouse brain. iNSCs showed sound in vivo long-term survival rates without graft overgrowths. The cells displayed a neural multilineage potential with a clear bias toward astrocytes and a permanent downregulation of progenitor and cell-cycle markers, indicating that iNSCs are not predisposed to tumor formation. Furthermore, the formation of synaptic connections as well as neuronal and glial electrophysiological properties demonstrated that differentiated iNSCs migrated, functionally integrated, and interacted with the existing neuronal circuitry. We conclude that iNSC long-term transplantation is a safe procedure; moreover, it might represent an interesting tool for future personalized regenerative applications.
Erscheinungsjahr
Zeitschriftentitel
Stem Cell Reports
Band
3
Zeitschriftennummer
3
Seite
423-431
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Hemmer K, Zhang M, van Wuellen T, et al. Induced Neural Stem Cells Achieve Long-Term Survival and Functional Integration in the Adult Mouse Brain. Stem Cell Reports. 2014;3(3):423-431.
Hemmer, K., Zhang, M., van Wuellen, T., Sakalem, M., Tapia, N., Baumuratov, A., Kaltschmidt, C., et al. (2014). Induced Neural Stem Cells Achieve Long-Term Survival and Functional Integration in the Adult Mouse Brain. Stem Cell Reports, 3(3), 423-431. doi:10.1016/j.stemcr.2014.06.017
Hemmer, K., Zhang, M., van Wuellen, T., Sakalem, M., Tapia, N., Baumuratov, A., Kaltschmidt, C., Kaltschmidt, B., Schoeler, H. R., Zhang, W., et al. (2014). Induced Neural Stem Cells Achieve Long-Term Survival and Functional Integration in the Adult Mouse Brain. Stem Cell Reports 3, 423-431.
Hemmer, K., et al., 2014. Induced Neural Stem Cells Achieve Long-Term Survival and Functional Integration in the Adult Mouse Brain. Stem Cell Reports, 3(3), p 423-431.
K. Hemmer, et al., “Induced Neural Stem Cells Achieve Long-Term Survival and Functional Integration in the Adult Mouse Brain”, Stem Cell Reports, vol. 3, 2014, pp. 423-431.
Hemmer, K., Zhang, M., van Wuellen, T., Sakalem, M., Tapia, N., Baumuratov, A., Kaltschmidt, C., Kaltschmidt, B., Schoeler, H.R., Zhang, W., Schwamborn, J.C.: Induced Neural Stem Cells Achieve Long-Term Survival and Functional Integration in the Adult Mouse Brain. Stem Cell Reports. 3, 423-431 (2014).
Hemmer, Kathrin, Zhang, Mingyue, van Wuellen, Thea, Sakalem, Marna, Tapia, Natalia, Baumuratov, Aidos, Kaltschmidt, Christian, Kaltschmidt, Barbara, Schoeler, Hans R., Zhang, Weiqi, and Schwamborn, Jens C. “Induced Neural Stem Cells Achieve Long-Term Survival and Functional Integration in the Adult Mouse Brain”. Stem Cell Reports 3.3 (2014): 423-431.

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