Metabolic flux pattern of glucose utilization by Xanthomonas campestris pv. campestris: prevalent role of the Entner-Doudoroff pathway and minor fluxes through the pentose phosphate pathway and glycolysis

Schatschneider S, Huber C, Neuweger H, Watt T, Pühler A, Eisenreich W, Wittmann C, Niehaus K, Vorhölter F-J (2014)
Molecular BioSystems 10(10): 2663-2676.

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Abstract
The well-studied plant pathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) synthesizes the biotechnologically important polysaccharide xanthan gum, which is also regarded as a virulence factor in plant interactions. In Xcc, sugars like glucose are utilized as a source to generate energy and biomass for growth and pathogenicity. In this study, we used [1-C-13]glucose as a tracer to analyze the fluxes in the central metabolism of the bacterium growing in a minimal medium. C-13-Metabolic flux analysis based on gas chromatography-mass spectrometry (GC-MS) confirmed the prevalent catabolic role of the Entner-Doudoroff pathway. Comparative nuclear magnetic resonance (NMR)-based isotopologue profiling of a mutant deficient in glycolysis gave evidence for a moderate flux via glycolysis in the wild-type. In addition to reconfirming the Entner-Doudoroff pathway as a catabolic main route, this approach affirmed a numerically minor but important flux via the pentose phosphate pathway.
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Schatschneider S, Huber C, Neuweger H, et al. Metabolic flux pattern of glucose utilization by Xanthomonas campestris pv. campestris: prevalent role of the Entner-Doudoroff pathway and minor fluxes through the pentose phosphate pathway and glycolysis. Molecular BioSystems. 2014;10(10):2663-2676.
Schatschneider, S., Huber, C., Neuweger, H., Watt, T., Pühler, A., Eisenreich, W., Wittmann, C., et al. (2014). Metabolic flux pattern of glucose utilization by Xanthomonas campestris pv. campestris: prevalent role of the Entner-Doudoroff pathway and minor fluxes through the pentose phosphate pathway and glycolysis. Molecular BioSystems, 10(10), 2663-2676.
Schatschneider, S., Huber, C., Neuweger, H., Watt, T., Pühler, A., Eisenreich, W., Wittmann, C., Niehaus, K., and Vorhölter, F. - J. (2014). Metabolic flux pattern of glucose utilization by Xanthomonas campestris pv. campestris: prevalent role of the Entner-Doudoroff pathway and minor fluxes through the pentose phosphate pathway and glycolysis. Molecular BioSystems 10, 2663-2676.
Schatschneider, S., et al., 2014. Metabolic flux pattern of glucose utilization by Xanthomonas campestris pv. campestris: prevalent role of the Entner-Doudoroff pathway and minor fluxes through the pentose phosphate pathway and glycolysis. Molecular BioSystems, 10(10), p 2663-2676.
S. Schatschneider, et al., “Metabolic flux pattern of glucose utilization by Xanthomonas campestris pv. campestris: prevalent role of the Entner-Doudoroff pathway and minor fluxes through the pentose phosphate pathway and glycolysis”, Molecular BioSystems, vol. 10, 2014, pp. 2663-2676.
Schatschneider, S., Huber, C., Neuweger, H., Watt, T., Pühler, A., Eisenreich, W., Wittmann, C., Niehaus, K., Vorhölter, F.-J.: Metabolic flux pattern of glucose utilization by Xanthomonas campestris pv. campestris: prevalent role of the Entner-Doudoroff pathway and minor fluxes through the pentose phosphate pathway and glycolysis. Molecular BioSystems. 10, 2663-2676 (2014).
Schatschneider, Sarah, Huber, Claudia, Neuweger, Heiko, Watt, Tony, Pühler, Alfred, Eisenreich, Wolfgang, Wittmann, Christoph, Niehaus, Karsten, and Vorhölter, Frank-Jörg. “Metabolic flux pattern of glucose utilization by Xanthomonas campestris pv. campestris: prevalent role of the Entner-Doudoroff pathway and minor fluxes through the pentose phosphate pathway and glycolysis”. Molecular BioSystems 10.10 (2014): 2663-2676.
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CeCaFDB: a curated database for the documentation, visualization and comparative analysis of central carbon metabolic flux distributions explored by 13C-fluxomics.
Zhang Z, Shen T, Rui B, Zhou W, Zhou X, Shang C, Xin C, Liu X, Li G, Jiang J, Li C, Li R, Han M, You S, Yu G, Yi Y, Wen H, Liu Z, Xie X., Nucleic Acids Res. 43(Database issue), 2015
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